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Partial VP2 sequencing of canine parvovirus (CPV) strains circulating in the state of Rio de Janeiro, Brazil: detection of the new variant CPV-2c BJM
Castro,T.X.; Costa,E.M; Leite,J.P.G.; Labarthe,N.V.; Cubel Garcia,R.C.N..
Canine parvovirus (CPV) is the most important enteric virus for dogs and it seems to be undergoing continuous evolution, generating new genetic and antigenic variants throughout the world. The aim of this study was to analyze the distribution of CPV variants from 1995 to 2009 and to investigate the circulation of the new variant CPV-2c in Rio de Janeiro, Brazil. In addition, the clinical features of CPV infection were also reported. After CPV laboratorial confirmation by HA/HI and PCR, thirty-two fecal samples were analyzed by sequencing a 583-bp fragment of the VP2 gene. One sample, collected in 2008 was typed as the new type CPV-2c. All samples from 1995 to 2003 were identified as "new CPV-2a". From 2004 to 2006, both "new CPV-2a" and CPV-2b were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CPV-2c; Sequence analysis; Enteritis; Rio de Janeiro; Brazil.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400031
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Isolation and characterization of canine parvovirus type 2c circulating in Uruguay Ciência Rural
Pintos,Andrea Blanc; Larrama,Cecilia Beatriz Negro; Baratta,Eduardo Enrique Reolon; Barthe,Mabel Beatriz Berois; Rodonz,Juan Ramón Arbiza.
This research reports the first CPV-2c isolation in cell culture (canine fibroma cell line A-72) in Uruguay. The isolates were obtained from 13 rectal swabs of Uruguayan dogs with parvovirosis. Samples were submitted to PCR with two sets of primers, restriction fragment length polymorphism (RFLP), partial sequencing of the gene encoding for VP2 capsid protein and phylogenetic characterization. The strain isolated was confirmed as CPV-2c. These results contribute to a better knowledge of CPV strains circulating in Uruguay and promote an evaluation of the efficacy of heterologous vaccines used to protect against the circulating strains.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine parvovirosis; CPV-2c; Vaccinated dogs.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000800022
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