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| Torres-Vázquez,José Antonio; Vázquez Flores,Felícitas; Montaldo,Hugo H; Ulloa-Arvizu,Raúl; Valencia Posadas,Mauricio; Gayosso Vázquez,Amanda; Alonso Morales,Rogelio Alejandro. |
| With the objective of estimating allele frequencies, and testing for population divergence for the CSN1S1 locus, genotypes of animals from five goat populations; Saanen (n = 97), Alpine (n = 81) Toggenburg (n = 92), local goats with external appearance similar to the Murciana-Granadina breed from Central Mexico (n = 26) and heterogeneous local animals denominated Mosaico Lagunero (n = 30), from Northern Mexico, were identified using PCR and Xmn1 PCR-RFLP methodology. For Saanen, Alpine and Toggenburg, the sum of E and F alleles had the largest frequencies (from 0.468 to 0.789), while for the groups local Murciana-Granadina and Mosaico Lagunero the sum of the most frequent allelic groups (A* and B*), were 0.385 and 0.533 respectively. Both local... |
| Tipo: Journal article |
Palavras-chave: CSN1S1; Gene frequencies; Genetic diversity; Goat milk; PCR-RFLP. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300007 |
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| Soares,Maria Amélia Menck; Rodrigues,Marcelo Teixeira; Mognol,Giuliana Patrícia; Ribeiro,Lucinéia de Fátima Chasko; Silva,José Luis da Conceição; Brancalhão,Rose Meire Costa. |
| Three different regions of the alpha s1-casein gene (CSN1S1) were investigated to determine the frequencies of major alleles for null, low, intermediate and high milk protein expression in a herd of dairy goats raised in the southeastern region of Brazil. Genomic DNA samples were obtained from leukocytes of 145 dairy goats and regions of interest in the gene were amplified through Polymerase Chain Reaction (PCR), then evaluated in both agarose (O and E allele) and polyacrylamide gels (F allele). For better characterization of the F allele, a PCR-RFLP (PCR-Restriction Fragment Length Polymorphism) study was performed employing the endonuclease XmnI. The allelic frequencies in the herd of 62 Saanen goats studied were: CSN1S1E = 0.35; CSN1S1F = 0.30; CSN1S1O1... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alpine; Capra hircus; CSN1S1; Genotyping; Milk proteins; Saanen. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009000600008 |
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