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	Provedor de dados Biol. Res. (2) Autor HIDALGO,CECILIA (2) ARACENA,PAULA (1) BARRIENTOS,GENARO (1) BELTRÁN,MARIANELA (1) DONOSO,PAULINA (1) SANCHEZ,GINA (1) Mais... Palavra-chave Calcium release kinetics (2) Ryanodine receptors (2) Sarcoplasmic reticulum (2) Calcium-binding proteins (1) Excitation-contraction coupling (1) Mg2+ inhibition (1) Redox state (1) S-nitrosoglutathione (1) Skeletal and cardiac muscle (1) Mais... Tipo do documento Journal article (2) Ano 2006 (1) 2002 (1) País Chile (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Redox regulation of calcium release in skeletal and cardiac muscle Biol. Res. HIDALGO,CECILIA; ARACENA,PAULA; SANCHEZ,GINA; DONOSO,PAULINA. In skeletal and cardiac muscle cells, specific isoforms of the Ryanodine receptor channels mediate Ca2+ release from the sarcoplasmic reticulum. These channels are highly susceptible to redox modifications, which regulate channel activity. In this work, we studied the effects of Ca2+ (endogenous agonist) and Mg2+ (endogenous inhibitor) on the kinetics of Ca2+ release from sarcoplasmic reticulum vesicles isolated from skeletal or cardiac mammalian muscle. Native skeletal vesicles exhibited maximal stimulation of release kinetics by 10-20 µM [Ca2+], whereas in native cardiac vesicles, maximal stimulation of release required only 1 µM [Ca2+]. In 10 µM [Ca2+], free [Mg2+] < 0.1 mM produced marked inhibition of release from skeletal vesicles but free [Mg2+]... Tipo: Journal article Palavras-chave: Redox state; Ryanodine receptors; Sarcoplasmic reticulum; Calcium release kinetics; Mg2+ inhibition; S-nitrosoglutathione. Ano: 2002 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200009 Fast kinetics of calcium dissociation from calsequestrin Biol. Res. BELTRÁN,MARIANELA; BARRIENTOS,GENARO; HIDALGO,CECILIA. We measured the kinetics of calcium dissociation from calsequestrin in solution or forming part of isolated junctional sarcoplasmic reticulum membranes by mixing calsequestrin equilibrated with calcium with calcium-free solutions in a stopped-flow system. In parallel, we measured the kinetics of the intrinsic fluorescence changes that take place following calcium dissociation from calsequestrin. We found that at 25ºC calcium dissociation was 10-fold faster for calsequestrin attached to junctional membranes (k = 109 s-1) than in solution. These results imply that calcium dissociation from calsequestrin in vivo is not rate limiting during excitation-contraction coupling. In addition, we found that the intrinsic fluorescence decrease for calsequestrin in... Tipo: Journal article Palavras-chave: Calcium-binding proteins; Ryanodine receptors; Sarcoplasmic reticulum; Calcium release kinetics; Excitation-contraction coupling; Skeletal and cardiac muscle. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300011 Registros recuperados: 2 Primeira ... 1 ... Última

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