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In vitro culture of Phyllanthus stipulatus (Euphorbiaceae) Rev. Bras. Bot.
CATAPAN,ELIZABETE; OTUKI,MICHEL FLEITH; VIANA,ANA MARIA.
An efficient micropropagation protocol was developed for the medicinal plant Phyllanthus stipulatus (Euphorbiaceae) using nodal segments for axillary shoot proliferation. Maximum multiplication rates (8-9 shoots per explant) was achieved on MS media supplemented with either 2.5-5.0 muM IBA. The best basal media for axillary shoot proliferation when 0.62 muM BA was supplemented were MS, MS/2 and AR (4-5 shoots per explant). Rooting was achieved with 100% of the microshoots on MS medium without growth regulators. Regenerated plants were successfully acclimatized and about 88% of plantlets survived under ex vitro conditions. Flowering was observed in 81% of the ex vitro grown plantlets after 12 weeks of acclimatization. High frequency callus initiation and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Shoot culture; Acclimatization; Ex-vitro flowering; Callus culture; Root culture.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042001000100003
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Improvement of friable callus production of Boerhaavia paniculata Rich and the investigation of its lipid profile by GC/MS Anais da ABC (AABC)
SOUZA,JOANNE M.M.; BERKOV,STRAHILL; SANTOS,ALBERDAN S..
In this study, a protocol to induce high amount of friable callus of Boerhaavia paniculata RICH and a lipidomics technique were applied to investigate the profile of lipids to relate to those present in the roots of this plant that presented anti-inflammatory activity in the crude hexane extract. The callus culture was induced from seeds in solidified Murashige and Skoog medium containing different amounts of glucose and different concentrations of 2,4-Dichlorophenoxyacetic acid. The explants were kept in a germination chamber at 30±2°C with a photoperiod of 16 h under light intensity of 27 µmol m–2 s–1 for 4 weeks. The best results for friable callus formation and development of the biomass were obtained in the treatment containing 2.26 µM 2.4-D and...
Palavras-chave: Anti-inflammatory lipids; Boerhaavia paniculata; Callus culture; Lipid profile; Medicinal plant.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000301015
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Alterations in core histone variant ratios during maize root differentiation, callus formation and in response to plant hormone treatment Biol. Res.
ALATZAS,ANASTASIOS; FOUNDOULI,ATHINA.
Although several histone variants have been studied in both animal and plant organisms, little is known about their distribution during processes that involve alterations in chromatin function, such as differentiation, dedifferentiation and hormone treatment. In this study we evaluated the ratio of each histone variant in each of the four core histone classes in the three developmental zones of maize (Zea mays L.) root and in callus cultures derived from them, in order to define possible alterations either during plant cell differentiation or dedifferentiation. We also evaluated core histone variant ratios in the developmental zones of roots treated with auxin and gibberellin in order to examine the effects of exogenously applied plant hormones to histone...
Tipo: Journal article Palavras-chave: Callus culture; Histone variant; Plant cell differentiation; Plant hormone; Zea mays.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400006
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Distribution of linker histone variants during plant cell differentiation in the developmental zones of the maize root, dedifferentiation in callus culture after auxin treatment Biol. Res.
ALATZAS,ANASTASIOS; SREBREVA,LJUBA; FOUNDOULI,ATHINA.
Although several linker histone variants have been studied in both animal and plant organisms, little is known about their distribution during processes that involve alterations in chromatin function, such as differentiation, dedifferentiation and hormone treatment. In this study, we identified linker histone variants by using specific anti-histone Hl antibodies. Each variant's ratio to total Hl in the three developmental zones of maize (Zea mays L.) root and in callus cultures derived from them was estimated in order to define possible alterations either during plant cell differentiation or during their dedifferentiation. We also evaluated linker histone variants' ratios in the developmental zones of maize roots treated with auxin in order to examine the...
Tipo: Journal article Palavras-chave: Callus culture; Linker histone; Plant cell differentiation; Plant hormone; Zea mays.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200010
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Search for alkaloids on callus culture of Passiflora alata BABT
Machado,Michelli Wesz; Stern Neto,Carlos; Salgado,Juliana; Zaffari,Gilmar; Barison,Andersson; Campos,Francinete Ramos; Corilo,Yuri Eberlim de; Eberlin,Marcos Nogueira; Biavatti,Maique Weber.
Preliminary work on Passiflora alata leaves failed to detect harmane alkaloids using LC. The aim of this work was to investigate the production of harmane alkaloids through the cell culture of P. alata, inducing its precursor (L-tryptophan). The leaf explants presented satisfactory results after disinfection, and the callus formation was initiated in MS media with adequate quantities of phytohormones. Sixty days after inoculation, calli were inoculated in the optimized semi-solid MS media, with and without the addition of L-tryptophan (50, 100, 200 mg/L) and kept in standard conditions for 90 days. Calli were collected on days 6, 16, 26, 36, and 90, followed by acid-base extraction, and analysed by LC. The results showed an absence of harmane, harmin,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Passiflora alata; Β-carboline alkaloids; Pyrazoles; Callus culture.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400020
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Comparative study of antibacterial and antifugal activity of callus culture and adult plants extracts from Alternanthera maritima (Amaranthaceae) BJM
Salvador,Marcos J.; Pereira,Paulo S.; França,Suzelei C.; Candido,Regina C.; Ito,Izabel Y.; Dias,Diones A..
The aim of this study was to evaluate the antibacterial and antifungal activity of callus culture (two different hormonal combination culture medium) and adult plants (two collect) extracts from Alternanthera maritima (Amaranthaceae) investigating the maintenance of antimicrobial activity in vivo and in vitro. The antibacterial and antifungal activity was determined by the agar-well diffusion method against thirty strains of microorganisms including Gram-positive and Gram-negative bacteria, yeasts and dermatophytes. All the organic crude extracts studied were bioactive. Extracts of aerial parts and roots of adult plants collected during the same period of years of 1995 and 1998 (Restinga de Maricá (RJ), collect 1 and 2) inhibited the growth of several...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alternanthera maritima; Adult plants; Callus culture; Biotechnology; Antimicrobial activity.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100022
Registros recuperados: 6
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