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	Provedor de dados PAB (2) Autor Chardulo,Luis Artur Loyola (2) Curi,Rogério Abdallah (2) Oliveira,Henrique Nunes de (2) Arrigoni,Mário De Beni (1) Assumpção,Mayra Elena Ortiz D' Avila (1) Fortes,Marina Rufino Salinas (1) Martins,Cyntia Ludovico (1) Silveira,Antonio Carlos (1) Silveira,Antônio Carlos (1) Mais... Palavra-chave Calpastatin gene (2) Bos indicus (1) Calpain gene (1) Meat tenderness (1) Meat texture (1) PCR-RFLP (1) SNP (1) Single nucleotide polymorphisms (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2009 (1) 2008 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Alternative genotyping method for the single nucleotide polymorphism A2959G (AF159246) of the bovine CAST gene PAB Curi,Rogério Abdallah; Chardulo,Luis Artur Loyola; Silveira,Antônio Carlos; Oliveira,Henrique Nunes de. The objective of this work was to genotype the single nucleotide polymorphism (SNP) A2959G (AF159246) of bovine CAST gene by PCR-RFLP technique, and to report its use for the first time. For this, 147 Bos indicus and Bos taurus x Bos indicus animals were genotyped. The accuracy of the method was confirmed through the direct sequencing of PCR products of nine individuals. The lowest frequency of the meat tenderness favorable allele (A) in Bos indicus was confirmed. The use of PCR-RFLP for the genotyping of the bovine CAST gene SNP was shown to be robust and inexpensive, which will greatly facilitate its analysis by laboratories with basic structure. Tipo: Info:eu-repo/semantics/article Palavras-chave: Calpastatin gene; Meat texture; PCR-RFLP; SNP. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000500014 Genetic polymorphisms related to meat traits in purebred and crossbred Nelore cattle PAB Curi,Rogério Abdallah; Fortes,Marina Rufino Salinas; Chardulo,Luis Artur Loyola; Silveira,Antonio Carlos; Arrigoni,Mário De Beni; Martins,Cyntia Ludovico; Assumpção,Mayra Elena Ortiz D' Avila; Oliveira,Henrique Nunes de. The objective of this work was to estimate the allelic and genotypic frequencies of CAST/XmnI, a calpastatin gene polymorphism, and CAPN530, a calpain 1 large subunit gene polymorphism, in different beef genetic groups (Nelore and Nelore x Bos taurus), and to investigate associations between these polymorphisms and carcass and meat traits. Three hundred animals - comprising 114 Nelore, 67 Angus x Nelore, 44 Rubia Gallega x Nelore, 41 Canchim, 19 Brangus three-way cross and 15 Braunvieh three-way cross- were genotyped by PCR-RFLP and phenotyped for rib-eye area (REA), back-fat thickness (BT), intramuscular fat (IF), shear force (SF) and myofibrillar fragmentation index (MFI). The occurrence of the two alleles of the CAST/XmnI and CAPN530 single nucleotide... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bos indicus; Calpain gene; Calpastatin gene; Meat tenderness; Single nucleotide polymorphisms. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009001200015 Registros recuperados: 2 Primeira ... 1 ... Última

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