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| Registros recuperados: 16 | |
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| Carvalho,A.F.; Silva,D.M.; Azevedo,S.S.; Piatti,R.M.; Genovez,M.E.; Scarcelli,E.. |
| Foram analisadas 80 amostras de sobrecoxas de frangos de corte resfriados provenientes de feiras livres e hipermercados do município de São Paulo, SP. Treze estirpes de Campylobacter spp. foram isoladas em 10 (12,5%) sobrecoxas, sendo cinco amostras originárias de feiras livres e cinco de hipermercados. Onze estirpes foram identificadas como Campylobacter jejuni e duas como Campylobacter coli. As 11 estirpes foram confirmadas como C. jejuni pela PCR do gene da hipuricase (hip), e destas, quatro (36,4%) apresentaram os três genes (cdtA, cdtB e cdtC) codificantes da toxina citoletal distensiva pela multiplex-PCR, sendo três estirpes provenientes de hipermercados e uma de feira livre. Observou-se a presença de estirpes virulentas de C. jejuni, portadoras do... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Frango de corte; Campylobacter jejuni; Toxina citoletal distensiva; PCR. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000500006 |
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| Fica,Alberto; Seelmann,Daniela; Porte,Lorena; Eugenin,Daniela; Gallardo,Ricardo. |
| Myopericarditis is an infrequent complication of acute diarrheal illness due to Campylobacter jejuni, and it has been mainly reported in developed nations. The first case detected in Chile - an upper-middle income country -, that is coincidental with the increasing importance of acute gastroenteritis associated to this pathogen, is described. Recognition of this agent in stools requires special laboratory techniques not widely available, and it was suspected when a young patient presented with acute diarrhea, fever, and chest pain combined with electrocardiogram (EKG) abnormalities and elevated myocardial enzymes. C. jejuni myopericarditis can easily be suspected but its detection requires dedicated laboratory techniques. |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Campylobacter jejuni; Myocarditis; Infectious diarrheal disease. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000300014 |
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| Gomes,Fabiane R.; Curcio,Bruna R.; Ladeira,Sílvia R.L.; Fernández,Heriberto; Meireles,Mário Carlos Araújo. |
| The aim of this study was to evaluate the occurrence of thermophilic Campylobacter species in broiler flocks from 26 small properties located near the campus of Federal University of Pelotas, RS, Brazil. A total of 404 chicken fecal samples were analyzed and after isolation, identification and biotyping was performed according to Lior´s scheme. Twenty one strains (5.2%) of Campylobacter jejuni biotype II were isolated from animals from seven properties (26.9%), with a variation from 3% to 34.6% among the positive properties. We also analyzed twelve C. jejuni isolates by polymerase chain reaction (PCR), using specific primers that generate a fragment of 402 pb. Our study allowed us to observe that chickens raised in non-industrial establishments without... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Campylobacter jejuni; Thermotolerant species; Diarrhea; Chicken. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300032 |
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| González-Hein,Gisela; Huaracán,Bernardo; García,Patricia; Figueroa,Guillermo. |
| Campylobacter jejuni isolates of different origins (bovine, broiler meat, human) were screened by polymerase chain reaction for the presence of 4 genes cdtB, cst-II, ggt, and virB11, previously linked to virulence such as adherence, invasion, colonization, molecular mimicry, and cytotoxin production. In addition, the isolates were screened for the presence of the global gene regulator csrA linked to oxidative stress responses, biofilms formation, and cell adhesion. All the C. jejuni isolates were positive for cdtB gene. The csrA gene was detected in 100% and 92% of C. jejuni isolates from human and animal origin and the virB11 gene was detected in 7.3% and 3.6% isolates from chicken and human respectively. All isolates from bovine were negative for the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Campylobacter jejuni; Virulence; Guillain Barré syndrome; Broiler; Bovine. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400028 |
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| Scarcelli,E.; Piatti,R.M.; Harakava,R.; Miyashiro,S.; Campos,F.R.; Souza,M.C.A.; Cardoso,M.V.; Teixeira,S.R.; Genovez,M.E.. |
| The objectives of the present study were the subtyping of Campylobacter jejuni subsp. jejuni strains obtained from humans and different animal species using PCR-RFLP, and the detection, by means of the same technique, of strains related to serotype PEN O19:LIO 7, the main C. jejuni serotype linked to Guillain-Barré Syndrome (GBS). Seventy C. jejuni strains isolated from human feces (n=33), primates (n=15), dogs (n=5), swine (n=2), bovines (n=1), abortion material from goats (n=2) and poultry carcasses (n=12), all collected in the state of São Paulo, were subtyped by means of PCR-RFLP of fla A gene, using restriction endonucleases Hae III, Afa I and Mbo I. Seven subtypes were observed when using the enzyme Hae III; eight when using Mbo I; and seven when... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Campylobacter jejuni; PCR-RFLP; Subtyping; Guillain-Barré Syndrome. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400029 |
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| Singh,Harkanwaldeep; Rathore,R.S; Singh,Satparkash; Cheema,Pawanjit Singh. |
| In the present study, the efficacy of polymerase chain reaction (PCR) based on mapA gene of C. jejuni was tested for detection of Campylobacter jejuni in naturally infected as well as spiked faecal and food samples of human and animal origin. Simultaneously, all the samples were subjected to the cultural isolation of organism and biochemical characterization. The positive samples resulted in the amplification of a DNA fragment of size ~589 bp in PCR assay whereas the absence of such amplicon in DNA extracted from E. coli, Listeria, Salmonella and Staphylococcus confirmed the specificity of the primers. Of randomly collected 143 faecal samples comprising human diarrheic stools (43), cattle diarrheic faeces (48) and poultry faecal swabs (52) only 4, 3 and 8,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Campylobacter jejuni; Isolation; PCR; Spiking. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100022 |
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| Scarcelli,Eliana; Costa,Elizabeth Oliveira da; Genovez,Margareth Élide; Cardoso,Maristela Vasconcellos; Bach,Erna Elizabeth; Torres,Ana Paula. |
| Electrophoretic protein profiles of Campylobacter jejuni subsp. jejuni strains isolated from feces of seven animal species, including man, were compared. Fourteen strains (two from each species) plus two human strains and the reference one, were ruptured by ultrasound and their total soluble proteins were analyzed by SDS-PAGE technique in a 12% polyacrylamide gel with computerized densitometric reading by the molecular analyst software. All the strains had bands in common that correspond to 45 and 66 Kda molecular weight. The disagreement corresponded to a 97 to 200 Kda molecular weight region. From the 17 strains, 13 (76.5%), were classified as biotype I, three (17.6%) as biotype II and one (5.8%) as biotype III. Since protein extracts were obtained from... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Campylobacter jejuni; SDS-PAGE; Biotyping. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400006 |
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| Paravisi,M; Laviniki,V; Bassani,J; Kunert Filho,HC; Carvalho,D; Wilsmann,DE; Borges,KA; Furian,TQ; Salle,CTP; Moraes,HLS; Nascimento,VP. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antimicrobial resistance; Campylobacter jejuni; GyrA; Mutation; Poultry; TetO. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000200323 |
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| Fonseca,BB; Ferreira Júnior,A; Santos,JP dos; Coelho,LR; Rossi,DA; Melo,RT; Mendonça,EP; Araújo,TG; Alves,RN; Beletti,ME. |
| Abstract Campylobacteriosis is a worldwide foodborne zoonosis disease caused by Campylobacter jejuni. This microorganism is considered a commensal bacterium in chicken hosts. C. jejuni produces epithelial cell modifications and induces a cytokine gene transcription innate immunity repertoire. In the present study, we describe the invasiveness, morphological cellular modifications, and transcript level expressions of innate immune cytokines from C. jejuni-inoculated chicken ileum explants. C. jejuni was internalized by epithelial ileum cells at 15 minutes postinoculation (p.i.) and was detected intracellularly for 4hs (p.i.). Inoculated explants displayed significant increases in cell height. C. jejuni induced a significant elevation of Transforming Growth... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Campylobacter jejuni; Chicken; Explants; TGF-b3; IL-1b. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000100063 |
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| Registros recuperados: 16 | |
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