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| Castilho,J.G.; Brandão,P.E.; Carnieli Jr,P.; Oliveira,R.N.; Macedo,C.I.; Peixoto,Z.M.P.; Carrieri,M.L.; Kotait,I.. |
| Eleven central-nervous-system samples collected from stray dogs between 2000 and 2004 were found positive by RT-PCR, which amplified a 480bp fragment of the N gene of canine distemper virus (CDV). Phylogenetic analysis based on partial N-gene sequences showed four major clusters. All dog strains segregated into cluster I, with a mean nucleotide identity of 95.8% and 95.6% with the Onderstepoort and Lederle vaccine strains, respectively. Cluster II contained all the raccoon-related strains, cluster III Orient strains and Cluster IV the Onderstepoort and Lederle vaccine strains, with a mean nucleotide identity of 99.7% between them. This is the first report of phylogenetic analysis of CDV strains in Brazil. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dog; Canine distemper virus; RT-PCR; Phylogeny. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000300016 |
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| Mazzu-Nascimento,Thiago; Donofrio,Fabiana Cristina; Bianchi,Beatriz Cutilak; Travensolo,Regiane de Fátima; Souza Junior,João Batista; Moraes,Daniel Angeli; Morbioli,Giorgio Gianini; Segato,Thiago Pinotti; Varanda,Laudemir Carlos; Carrilho,Emanuel. |
| ABSTRACT Paper-based devices present low-cost and are versatile, making them very attractive for clinical analysis. To manufacture those devices wax patterns are printed on paper surface and upon heating the wax permeates through the entire thickness of the paper, creating hydrophobic barriers that delimit test areas. Antibodies produced in rabbits against canine distemper virus (CDV) were physically adsorbed on the surface of gold nanoparticles (AuNPs) and incubated with CDV viral antigens, forming the immunocomplex. Anti-CDV antibodies were immobilized into the microchannels by physical adsorption, forming the test region. The test solution containing conjugated AuNPs was applied at the bottom of the microchannel and it was eluted with a phosphate buffer... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Paper-based devices; Wax printing; Point-of-care testing; Gold nanoparticles; Canine distemper virus. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100318 |
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