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| LODATO,PATRICIA; ALCAÍNO,JENNIFER; BARAHONA,SALVADOR; RETAMALES,PATRICIO; JIMÉNEZ,ANTONIO; CIFUENTES,VÍCTOR. |
| The expression, at the mRNA level, of carotenoid biosynthetic genes from Xanthophyllomyces dendrorhous was studied by RT-PCR. The experimental conditions for the RT-PCR assay were standardized to quantify the relative transcript levels of idi, crtE, crtYB and crtI genes. This work attempted to correlate astaxanthin production with the transcript levels of carotenogenic genes in a wild-type strain (UCD 67-385) and two overproducer deregulated strains (atxS1 and atxS2). At 3 day cultures, the wild-type strain contained higher transcript levels from the crtE and crtYB genes on minimal medium than on rich medium. Similarly, carotenoid production was higher on minimal medium than on rich medium. However, carotenoid production in the atxS1 and atxS2 strains was... |
| Tipo: Journal article |
Palavras-chave: Carotenoid biosynthesis; Overproducer strains; Xanthophyllomyces dendrorhous; Phaffia rhodozyma. |
| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100009 |
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| Pizarro,Lorena; Stange,Claudia. |
| Carotenoids are colored terpenes synthesized in plants, algae and some yeasts and bacteria. In plants and algae, these lipophilic molecules exert functional roles inhormonesynthesis, photosynthesis, photomorphogenesis and photoprotection. Additionally, they possess antioxidant properties and act as scavengers of reactive oxygen species. During the past decade almost all of the carotenogenic genes have been identified by molecular, genetic and biochemical approaches in the Arabidopsis thaliana model system. Carotenoid biosynthesis in plants is highly regulated, although all of the processes involved have not yet been identified. In this work, we review the mechanisms involved in the light-mediated regulation of carotenoid biosynthesis and the effect of... |
| Tipo: Journal article |
Palavras-chave: Carotenoid biosynthesis; Gene expression; Light regulation; Plants. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202009000200001 |
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