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Estimation of genetic distance among genotypes of caraway (Carum carvi L.) using RAPD-PCR Agronomy
Seidler-Łożykowska, Katarzyna; Institute of Natural Fibres and Medicinal Plants; Kuczyńska, Anetta; Polish Academy of Sciences; Mikołajczyk, Katarzyna; Plant Breeding and Acclimatization Institute; Nowakowska, Joanna; Plant Breeding and Acclimatization Institute; Bocianowski, Jan; Poznań University of Life Sciences.
In order to estimate genetic diversity among starting materials and breeding strains of caraway, a collection of 17 accessions from botanical gardens in Europe, two cultivars ‘Rekord’ and ‘Kończewicki’, and four own breeding strains were analyzed by RAPD-PCR. The representative samples, each of five individual plants of accession, cultivar or strain, were taken from young rosette leaves. Forty of Genset Oligos RAPD primers were used for analysis and eight of them produced clear and reproducible banding patterns. In total, 62 banding patterns were obtained revealing 23 polymorphic bands, whereas the number of polymorphic bands ranged from two to four for one primer. The GS12 and GS43 primers generated two polymorphic bands, while each of the GS8, GS21,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carum carvi L.; Genetic distance; Genotype; RAPD.
Ano: 2014 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/18197
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