|
|
|
|
|
Zhang,Xiaoyong; Zhou,Jinyan; Fu,Wen; Li,Zhidong; Zhong,Juan; Yang,Jie; Xiao,Liang; Tan,Hong. |
Response surface methodology (RSM) was used for statistical optimization of jiean-peptide (JAA) production by Bacillus subtilis ZK8 cells adsorbed on wood chips to form a novel fermentation system. The Plackett-Burman design was used in the first step to evaluate the effects of eight factors, including six fermentation medium components and two cell adsorption conditions. Among the variables screened, soybean meal hydrolysate (SMH) and MgSO4·7H2O in the fermentation medium had significanteffects on JAA production. In the second step, the concentrations of SMH and MgSO4·7H2O were further optimized using central composite designs and response surface analysis. The optimized concentration of SMH and MgSO4·7H2O was 24% (v/v) and 0.38% (w/v), respectively,... |
Tipo: Journal article |
Palavras-chave: Bacillus subtilis ZK8; Cell adsorption; Jiean- peptide; Response surface methodology; Wood chips. |
Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400006 |
| |
|
|
Cheirsilp,Benjamas; Kitcha,Suleeporn; Maneerat,Supasilp. |
The kinetic characteristics of β-cyclodextrin production by a cyclodextrin glycosyltransferase (CGTase) produced by Bacillus sp. C26, a new isolate from a soil sample was investigated. Considering highest yield and initial production rate of β-cyclodextrin, among the starches examined, soluble starch, tapioca starch, sago starch, corn starch and rice starch, tapioca starch was the best substrate for this CGTase. The optimum temperature for tapioca starch gelatinization prior to its use as a substrate for β-cyclodextrin production was 65ºC. The yield and initial production rate of β-cyclodextrin increased with increasing starch concentration up to 6% and an enzyme concentration up to 48 U/g-starch. The kinetic parameters... |
Tipo: Journal article |
Palavras-chave: Bacillus subtilis ZK8; Cell adsorption; Jiean- peptide; Response surface methodology; Wood chips. |
Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400004 |
| |
|
|
|