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New tools to study biophysical properties of single molecules and single cells Anais da ABC (AABC)
Rocha,Márcio S.; Mesquita,Oscar N..
We present a review on two new tools to study biophysical properties of single molecules and single cells. A laser incident through a high numerical aperture microscope objective can trap small dielectric particles near the focus. This arrangement is named optical tweezers. This technique has the advantage to permit manipulation of a single individual object. We use optical tweezers to measure the entropic elasticity of a single DNA molecule and its interaction with the drug Psoralen. Optical tweezers are also used to hold a kidney cell MDCK away from the substrate to allow precise volume measurements of this single cell during an osmotic shock. This procedure allows us to obtain information about membrane water permeability and regulatory volume increase....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Optical tweezers; Defocusing microscopy; Single molecules; Macrophages; Water transport; Cell motility.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652007000100003
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Apigenin inhibits cell migration through MAPK pathways in human bladder smooth muscle cells Biocell
Qingxin,Liu; Xianggui,Chen; Guolin,Yang; Xuewen,Min; Maoxian,Deng.
Apigenin, a nonmutagenic flavonoid, has been shown to possess free radical scavenging activities, anticarcinogenic properties, antioxidant and anti-inflammatory effects. Recently, apigenin was reported to cause gastric relaxation in murine. To assess possible effects of apigenin on migration of bladder smooth muscle (SM) cell, we isolated SM cells from peri-cancer tissue of human bladder and established a cell model that was capable to overexpress transiently MEKK1 (MEK kinase 1). Results showed that overexpression of active human MEKK1 by adenoviruses infection induced migration of human bladder smooth muscle (hBSM) cells and phosphorylation of MAPKs, ERK, JNK and p38, which are the downstream molecules of MEKK1. Then, hBSM cell overexpressing MEKK1 were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Flavonoid; Cell motility; MAP kinase.
Ano: 2011 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452011000300002
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