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| Registros recuperados: 31 | |
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| Sriariyanun,Malinee; Tantayotai,Prapakorn; Yasurin,Patchanee; Pornwongthong,Peerapong; Cheenkachorn,Kraipat. |
| Background: Lignocellulosic biomass is a renewable, abundant, and inexpensive resource for biorefining process to produce biofuel and valuable chemicals. To make the process become feasible, it requires the use of both efficient pretreatment and hydrolysis enzymes to generate fermentable sugars. Ionic liquid (IL) pretreatment has been demonstrated to be a promising method to enhance the saccharification of biomass by cellulase enzyme; however, the remaining IL in the hydrolysis buffer strongly inhibits the function of cellulase. This study aimed to isolate a potential IL-tolerant cellulase producing bacterium to be applied in biorefining process. Result: One Bacillus sp., MSL2 strain, obtained from rice paddy field soil was isolated based on screening of... |
| Tipo: Journal article |
Palavras-chave: Biorefinery; Cellulase; Ionic liquid; Lignocellulosic biomass. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000100004 |
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| Villena,Gretty K; Gutiérrez-Correa,Marcel. |
| Lignocellulolytic enzyme production by Aspergillus niger was compared both in submerged fermentation (SF) and biofilm fermentation (BF) at varying water activities. Maximal filter paper activity, endoglucanase and xylanase activities were much higher in BF (2.96, 4.7 and 4.61 IU ml-1, respectively) than in SF cultures (1.71, 1.31 and 2.3 IU ml-1, respectively) but biomass yields were lower in BF than in SF (0.338 g g-1 and 0.431 g g-1, respectively). In the presence of 20% ethylene glycol (a w = 0.942) the enzyme activities decreased in both systems but BF still had higher levels (1.0, 1.0 and 2.6 IU ml-1, respectively) than SF cultures (0.6, 0.7 and 1.5 IU ml-1, respectively). An increase in xylanase specific activity of more than 2 fold (from 4.2 to 10.2... |
| Tipo: Journal article |
Palavras-chave: Biofilm; Cellulase; Ethylene glycol; Water activity; Xylanase. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100012 |
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| Lin,Ling; Kan,Xianzhao; Yan,Hao; Wang,Danni. |
| The gram-positive spore-forming bacteria, Bacillus thuringiensis (Bt) strains produced novel cellulases which could liberate glucose from soluble cellulose, carboxymethyl cellulose (CMC), and insoluble crystalline cellulose. The maximal cellulase activities were obtained after 60 hrs incubation at 28ºC in a LB broth medium with 1% CMC. Maximum CMCase activities were got at 40ºC and pH 4.0, respectively, and more than 50% of its maximal activity was retained at 40-60ºC for 1 hr, while approximately 40% of its maximal activity was also retained after incubating at 70ºC for 1 hr. Most metal ions and reagents such as Ca2+, Mg2+, Cd2+, Pb2+, Zn2+, Cu2+, EDTA, and SDS inhibited the enzyme activities, but K+ and Mn2+ activated the activities. The enzymes from... |
| Tipo: Journal article |
Palavras-chave: Bacillus thuringiensis; Cellulase; Cellulose-degrading; Characterization. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300002 |
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| El-Gioushy,Sherif Fathy Eid El-Sayed; Kareem,Abdul; Baiea,Mohamed Hemdan Mohamed. |
| Abstract To isolate protoplast, a pre-treatment was completed with the order to reduce and identify the phenolic contents round the year to encourage the isolation of protoplasts. Protoplasts from in vivo mesophyll leaves of apple cultivar “Anna” was isolated from 15 days old leaves by plasmolying in medium containing 90 g L-1 mannitol for half hour, then 130 g L-1 mannitol for half hour. Then using enzymatic mixture involving (1.5% cellulase + 0.5% pectianase + 1.5% Macrozyme) Prior to isolation. Anyhow, diverse factors, for example, Osmotic pressure, incubation period, sieve pore size, centrifugation period and hormonal balance was estimated using the techniques for isolation. The quantity of cells was computed as the quantity of cells per square on... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apple; Hybridization; Cellulase; Pectianase; Macrozyme. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000400803 |
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| Garcia,Martha Regina Lucizano; Sampaio,Alexandre Amstalden Moraes; Nahas,Ely. |
| The objective of this work was to study the effect of grazing systems: rotational, continuous and the absence of cattle, in pastures of Brachiaria brizantha on microbial activity (respiratory, potential nitrification, solubilizing, and enzymatic) and on the chemical attributes (contents of organic carbon, total nitrogen, organic phosphorus, and moisture) of Eutrustox soil over one year. Eight Canchim breed animals were used. The samples were collected in the four seasons from 2005 to 2006. The average of organic C, organic P, and moisture contents, as well as potential nitrification and solubilizing activity and urease and acid phosphatase activities were significantly higher in the spring and/or summer than in autumn and/or winter. The total nitrogen... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cellulase; Microbial respiratory activity; Phosphatase; Potential nitrification; Urease. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000700024 |
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| Vigne,Gabriela Letícia Delai; Neumann,Mikael; Santos,Leslei Caroline; Dochwat,André; Venancio,Bruno José; Heker Junior,Julio Cezar; Ueno,Robson Kyoshi; Souza,André Martins de. |
| ABSTRACT The objective of this study was to evaluate the production performance and carcass traits of feedlot steers fed a high-energy diet supplemented with doses of an enzyme complex (0, 2.5, 5.0, and, 7.5 g animal−1 day−1). Thirty-two ½ Angus ½ Nellore crossbred steers at an average age of 12 months and an average initial weight of 422 kg, were kept in a feedlot for 77 days. The roughage-free diet was composed of a mixture of whole corn grain and a protein-mineral-vitamin mix at a constant ratio of 85:15, on a dry matter basis. A completely randomized block design was adopted, consisting of four treatments and four replicates, in which each replicate was represented by a stall with two animals. Each gram of product added to the diet led to a decrease of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cellulase; Digestibility; Feed intake; Non-carcass component; Ultrasonography. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100612 |
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| Comlekcioglu,Ugur; Gunes,Merva; Altun,Hanifi; Ekiz,Dilek Ozgun; Aygan,Ashabil. |
| ABSTRACT Rumen fungi inhabit the gastro-intestinal tract of ruminants and the most non-ruminant herbivores. Rumen fungi produce highly active plant cell wall degrading enzymes, therefore they have gained scientific interest. In this study, genes encoding xylanase (xynA-7) and cellulase (celA-5) were amplified from Neocallimastix sp. GMLF7 and Orpinomyces sp. GMLF5, respectively, and expressed in Escherichia coli. XynA-7 was found to be active only on xylan, however CelA-5 had activity both on carboxymethyl cellulose and lichenan. Lichenase activity of CelA-5 was found to be higher than carboxymethyl cellulase activity. The optimal conditions were at pH 6.0 and 40 °C for CelA-5 and at pH 6.5 and 50 °C for XynA-7. A coexpression vector was constructed to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rumen fungi; Xylanase; Cellulase; Coexpression; E. coli. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100407 |
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| Singh,Jagdish; Kaur,Pawandeep. |
| The aim of this work was to isolate the potent bacterial strains for the production of cellulose enzyme. A total 30 bacterial isolates showed positive results for the cellulase production but highest enzyme activity was shown by isolate JS 14. From the morphological and biochemical reactions, the isolate was identified as Bacillus sp. Cellulase production was studied by this strain using response surface methodology (RSM). A central composite design (CCD) quadratic response surface was applied to explicate the parameters that significantly affected cellulase production in solid substrate fermentation (SSF). The wheat bran concentration and incubation period were significant factors. The process parameters optimized with response surface methodology was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cellulase; Response surface methodology; Optimization. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400004 |
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| Moretti,Marcia M. S.; Bocchini-Martins,Daniela A.; Silva,Roberto Da; Rodrigues,André; Sette,Lara D.; Gomes,Eleni. |
| Twenty-seven thermophilic and thermotolerant fungal strains were isolated from soil, decaying organic matter and sugarcane piles based on their ability to grow at 45ºC on medium containing corn straw and cardboard as carbon sources. These fungi were identified in the genera Aspergillus, Thermomyces, Myceliophthora, Thermomucor and Candida. The majority of the isolated strains produced xylanase and cellulases under solid state fermentation (SSF). The highest cellulase and xylanase productions were obtained by the cultivation of the strains identified as Aspergillus fumigatus M.7.1 and Myceliophthora thermophila M.7.7. The enzymes from these strains exhibited maximum activity at pH 5.0 and at 60 and 70ºC. The endo-glucanase from A. fumigatus was stable from... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Thermophilic fungi; Solid-state fermentation; Xylanase; Cellulase. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300032 |
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| Acharya,Somen; Chaudhary,Anita. |
| Most of the potential bioprospecting is currently related to the study of the extremophiles and their potential use in industrial processes. Recently microbial cellulases find applications in various industries and constitute a major group of industrial enzymes. Considerable amount of work has been done on microbial cellulases, especially with resurgence of interest in biomass ethanol production employing cellulases and use of cellulases in textile and paper industry. Most efficient method of lignocellulosic biomass hydrolysis is through enzymatic saccharification using cellulases. Significant information has also been gained about the physiology of thermophilic cellulases producers and process development for enzyme production and biomass... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lignocelluloses; Bioethanol; Cellulase; Thermophiles. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300001 |
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| Han,Lirong; Feng,Juntao; Zhang,Shuangxi; Ma,Zhiqing; Wang,Yonghong; Zhang,Xing. |
| The efficiency of enzymatic hydrolysis of cellulose can be improved by various pretreatments of the substrate. In order to increase the efficiency of enzymatic saccharification of the wheat straw, we determined the effect of different pretreatments on the physical structure, chemical components and enzymatic saccharification of wheat straw. Our results showed that combination of grinding and sodium hydroxide (NaOH) treatment had high effect on the enzymatic hydrolysis of wheat straws. The optimal pretreatment condition was to grind the wheat straws into the sizes of 120 meshes followed by treatment with 1.0% NaOH for 1.5 h (121°C/15psi). Under this condition, the cellulose content of wheat straw was increased by 44.52%, while the content of hemicellulose... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cellulase; Cellulose; Fermentation; Hydrolysis; Lignocellulosic; Pretreatment. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100006 |
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| Registros recuperados: 31 | |
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