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| Zaghi Júnior,Lienine Luiz; Lopes,Ana Daniela; Cordeiro,Fábio Aparecido; Colla,Itaruã Machri; Bertéli,Míria Benetati Delgado; Valle,Juliana Silveira do; Linde,Giani Andrea; Colauto,Nelson Barros. |
| Abstract Agaricus subrufescens is a basidiomycete which is studied because of its medicinal and gastronomic importance; however, less attention has been paid to its preservation. This study aimed to evaluate the effect of sucrose addition to substrate and cryotube on the viability of Agaricus subrufescens cryopreserved at -20 °C and at -75 °C for one and two years. Zero, 10% or 20% sucrose was added to potato dextrose agar or wheat grain. The mycelia were cryopreserved in the absence of cryoprotectant or with sucrose solutions at 15%, 30% or 45%. After one or two years at -75 °C or at -20 °C, mycelia were thawed and evaluated about viability, initial time of growth, colony diameter and genomic stability. Cryopreservation at -20 °C is not effective to keep... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Preservation; Freeze; Cereal grain; Monosaccharide; Agaricus blazei. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200370 |
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