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Molecular diagnosis of cerebral toxoplasmosis: comparing markers that determine Toxoplasma gondii by PCR in peripheral blood from HIV-infected patients BJID
Mesquita,Rafael Tonini; Vidal,José Ernesto; Pereira-Chioccola,Vera Lucia.
As cerebral toxoplasmosis is the most common cerebral focal lesion in AIDS patients, this study evaluated three PCR markers for diagnosis, since some limitations remain present, such as low parasite levels in some clinical samples. The molecular markers were B22-B23 and Tg1-Tg2 (based on the B1 gene) and Tox4-Tox5 (non-coding fragment, repeated 200-300-fold). DNA samples from 102 AIDS patients with previously known diagnosis were analyzed. The cerebral toxoplasmosis group was constituted of DNA extracted from the blood of 66 AIDS patients, which was collected before or until the third day of the therapy for toxoplasmosis. DNA from the blood of 36 AIDS patients with other neurologic opportunistic infections was used as control group. Sensitivities of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: AIDS; Cerebral toxoplasmosis; Molecular diagnosis; PCR markers.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000400006
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Ventriculitis: a rare case of primary cerebral toxoplasmosis in AIDS patient and literature review BJID
Cota,Gláucia Fernandes; Assad,Elisa Caroline Pereira; Christo,Paulo Pereira; Giannetti,Alexandre Varella; Santos Filho,José Augusto Malheiros dos; Xavier,Marcelo Antonio Pascoal.
Cerebral toxoplasmosis remains the most important neurological opportunistic infection and the most common cause of intracerebral mass lesion in patients with acquired immunodeficiency syndrome (AIDS). We report a case of an adult AIDS patient with an atypical pattern of toxoplasma encephalitis, presenting with ventriculitis and obstructive hydrocephalus without any focal parenchymal lesion.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Cerebral toxoplasmosis; Ventriculitis; Hydrocephalus; AIDS.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000100022
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Neurotoxoplasmosis diagnosis for HIV-1 patients by real-time PCR of cerebrospinal fluid BJID
Nogui,Fábio Luís Nascimento; Mattas,Sandro; Turcato Júnior,Gilberto; Lewi,David Salomão.
Encephalitis caused by Toxoplasma gondii is the most common cause of central nervous system damage in patients with acquired immunodeficiency syndrome (AIDS). Toxoplasma may infect any of the brain cells, thus leading to non-specific neurotoxoplasmosis clinical manifestations including focused or non-focused signs and symptoms of central nervous system malfunction. Clinical development ranges from insidious display during weeks to experiencing acute general confusion or ultimately fatal onset. Cerebral toxoplasmosis occurs in advanced stages of immunodeficiency, and the absence of anti-toxoplasmosis antibodies by the immunofluorescence method does not allow us to rule out its diagnosis. As specific therapy begins, diagnosis confirmation is sought through...
Tipo: Info:eu-repo/semantics/article Palavras-chave: AIDS; Cerebral toxoplasmosis; Real time polymerase chain reaction.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000100006
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Importance of high IgG anti-Toxoplasma gondii titers and PCR detection of T. gondii DNA in peripheral blood samples for the diagnosis of AIDS-related cerebral toxoplasmosis: a case-control study BJID
Vidal,José E; Diaz,Adrián Vladimir Hernández; Oliveira,Augusto César Penalva de; Dauar,Rafi Felicio; Colombo,Fabio Antonio; Chioccola,Vera Lucia Pereira.
BACKGROUND: Cerebral toxoplasmosis (CT) continues to cause significant morbidity and mortality in human immunodeficiency virus (HIV)-infected patients in Brazil. In clinical practice, the initial diagnosis is usually presumptive and alternative diagnosis tools are necessary. Our objective was to evaluate whether the detection of high titers of IgG anti-Toxoplasma gondii and T. gondii DNA in blood samples are associated with the diagnosis of CT. METHODS: In this case-control study we included 192 patients with HIV-1 infection: 64 patients with presumptive CT (cases) and 128 patients with other diseases (controls). Blood samples to perform indirect immunofluorescense reaction (IFI) to detect anti-T. gondii IgG antibodies and polymerase chain reaction (PCR)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Toxoplasmosis; Cerebral toxoplasmosis; Polymerase chain reaction; Diagnosis; Acquired immunodeficiency syndrome; Brazil.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400009
Registros recuperados: 4
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