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| Mattos,Adriana Tonani de; Freitas,Luciana Bueno de; Lima,Bettina Moulin Coelho; Miranda,Angélica Espinosa; Spano,Liliana Cruz. |
| Given the causal relationship between specific types of HPV with cervical cancer and precursor lesions, it is important to identify the viral type involved. The aim of this study is to access the prevalence of HPV types in HIV seropositive and seronegative women. Accordingly, 77 HPV positive cervical samples were obtained from 284 women (seropositive (n=112) and seronegative (n=172) for HIV) who attended a Sexually Transmitted Infection clinic, in Vitoria, Southeastern Brazil. Viral DNA was amplified by PCR using MY09/MY11 degenerated primers and the genotyping was performed by Restriction Fragment Length Polymorphism. Seventy five out of the 77 HPV samples were genotyped: 6, 11, 13, 16, 18, 26, 31, 31b, 32, 33, 34, 35, 52, 53, 55, 56, 58, 59, 61, 62, 64,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human papillomavirus (HPV); HIV seropositive and seronegative women; RFLP; Genotyping; Cervical secretion. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200047 |
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| Spano,Liliana Cruz; Ferreira,Mônica Simões Rocha; Almeida,Marilda Santos; Nascimento,Jussara Pereira do; Leite,José Paulo Gagliardi. |
| Human cytomegalovirus (HCMV) displays genetic variability in several regions, supposed to be related with strain-specific tissue tropism and immunopathogenesis. Based on sequence variation in the UL55 gene that encodes gB glycoprotein, HCMV strains can be assigned to one of four genotypes. Previous studies have addressed gB genotyping mostly by investigating strains derived from immunosuppressed patients, sometimes without previous knowledge about genotype distribution in a geographic area. The present study verified the distribution of HCMV gB genotypes of strains obtained from immunocompetent women at Vitória City, Espírito Santo State, Southeastern, Brazil. The HCMV genome was extracted from their cervical secretion, fetal and maternal placenta tissues... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytomegalovirus; RFLP; GB genotypes; Cervical secretion; Placenta tissues. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300008 |
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