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| Xu,HY; Tang,H; Pan,ZX; Li,L; Han,CC; Liu,HH; He,H; Kang,B; Hu,JW; Xia,L; Wang,Y; Wang,JW. |
| ABSTRACT The carbohydrate response element-binding protein (ChREBP) is an important nuclear factor that regulates glycolysis and de novo lipogenesis. However, the role of ChREBP in fatty liver development in geese remains unclear. In order to understand the function of ChREBP in lipid metabolism of geese, we first cloned the complete cDNA of the ChREBP of the Sichuan White goose (Anser cygnoides) using RT-PCR, 5’ RACE and 3’ RACE, and analyzed goose ChREBP expression in nine different tissues using real-time PCR technology. The results showed that the goose ChREBP CDS consists of 945bp nucleotides that encode 314 amino acids, and the sequence has high similarities with the swan goose (Anser cygnoides domesticus) and duck (Anas platyrhynchos) sequences,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ChREBP; Expression profile; Molecular cloning; RACE; Sichuan White Goose. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000400615 |
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