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Molecular characterization of Chaetomium species using URP-PCR Genet. Mol. Biol.
Aggarwal,Rashmi; Sharma,Vandana; Kharbikar,Lalit L.; Renu,.
Chaetomium spp. are common colonizers of soil and cellulose-containing substrates. Seventeen isolates of Chaetomium spp., which included 15 isolates of C. globosum and one each of C. reflexum and C. perlucidum , were genetically characterized with universal rice primers (URP - primers derived from DNA repeat sequences in the rice genome) using polymerase chain reaction (URP-PCR). Out of the 12 URP's used in the study, nine primers were effective in producing polymorphic fingerprint patterns from DNA of Chaetomium spp . Analysis of the entire fingerprint profile using the unweighted pair-group method with arithmetic averages (UPGMA) clearly differentiated C. globosum isolates from C. perlucidum and C. reflexum . One of the primers, URP-2R,...
Palavras-chave: Chaetomium globosum; Chaetomium reflexum; Chaetomium perlucidum; Genetic variability; URP-PCR.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500023
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Antagonism and modes of action of Chaetomium globosum species group, potential biocontrol agent of barley foliar diseases Boletín de la Sociedad
Moya,Paulina; Pedemonte,Debora; Amengual,Susana; Franco,Mario E. E; Sisterna,Marina N.
"Net blotch" (Drechslera teres) and "Bipolaris spot blotch" (Bipolaris sorokiniana) are foliar diseases of barley. Biological control is currently considered as an efficient alternative to chemical management of these plant diseases. The aim of the present study was to identify 2 isolates of Chaetomium (C2 and C5), endophytics on barley seedlings and to study the in vitro interactions with D. teres and B. sorokiniana, isolated from seeds of the same host. Cultural and morphological characterization of all microorganisms was done. In addition, molecular characterization of Chaetomium spp. was conducted and dual culture tests were carried out to find, by microscopic observations, the effects of the antagonist on the morphology of the pathogens. The results...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bipolaris sorokiniana; Chaetomium globosum; Drechslera teres; Antibiosis; Endophyte; Mycoparasitism.
Ano: 2016 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722016000400001
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Endophytic fungus strain 28 isolated from Houttuynia cordata possesses wide-spectrum antifungal activity BJM
Pan,Feng; Liu,Zheng-Qiong; Chen,Que; Xu,Ying-Wen; Hou,Kai; Wu,Wei.
Abstract The aim of this paper is to identify and investigate an endophytic fungus (strain 28) that was isolated from Houttuynia cordata Thunb, a famous and widely-used Traditional Chinese Medicine. Based on morphological methods and a phylogenetic analysis of ITS sequences, this strain was identified as Chaetomium globosum. An antifungal activity bioassay demonstrated that the crude ethyl acetate (EtOAc) extracts of strain 28 had a wide antifungal spectrum and strong antimicrobial activity, particularly against Exserohilum turcicum (Pass.) Leonard et Suggs, Botrytis cinerea persoon and Botrytis cinerea Pers. ex Fr. Furthermore, the fermentation conditions, extraction method and the heat stability of antifungal substances from strain 28 were also studied....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic fungi; Houttuynia cordata Thunb.; Chaetomium globosum; Antifungal activity; Process parameter.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200480
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New production process of the antifungal chaetoglobosin A using cornstalks BJM
Jiang,Cheng; Song,Jinzhu; Zhang,Junzheng; Yang,Qian.
Abstract Chaetoglobosin A is an antibacterial compound produced by Chaetomium globosum, with potential application as a biopesticide and cancer treatment drug. The aim of this study was to evaluate the feasibility of utilizing cornstalks to produce chaetoglobosin A by C. globosum W7 in solid-batch fermentation and to determine an optimal method for purification of the products. The output of chaetoglobosin A from the cornstalks was 0.34 mg/g, and its content in the crude extract was 4.80%. Purification conditions were optimized to increase the content of chaetoglobosin A in the crude extract, including the extract solvent, temperature, and pH value. The optimum process conditions were found to be acetone as the extractant, under room temperature, and at a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chaetoglobosin A; Cornstalk; Purification; Stability; Chaetomium globosum.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300410
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