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Excretion Products of Shigella dysenteriae and Apoptotic Cell Death on Chick Embryo Muscle Tissue International Journal of Morphology
Alvarez,Marco; Urbina,Gidalia; Müller,Claudia; Perdomo,Lourdes.
The aim of this study was to evaluate the acute cell injury of excretion products present in culture filtrate from Shigella dysenteriae in both whole lower limb of chick embryo ex vivo and myoblasts cells developed in hanging-drop cultures in vitro. Three controls were defined: a) Tyrode's solution b) brain-heart broth infusion (CCC) and c) supernatant not toxigenic of E.coli 0157:H7. Shigella dysenteriae were culture for 24 hours and the excretion products were obtained after centrifugation of the culture. After lh of treatment, the morphologic changes in limbs treated with raw filtrate were evaluated through histopathological examination of sections stained with hematoxylin-eosin (H&E-stain) and Gomori's trichrome by image analysis techniques....
Tipo: Journal article Palavras-chave: Shigella dysenteriae; Shiga toxin; Chick embryo; Muscle tissue; Apoptosis.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000300024
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Biological Effects Produced by Intense Pulsed Light (Xe-Cl) on the Cartilage of the Tongue Chick Embryo Using Various Filters International Journal of Morphology
Avila,R. E; Samar,M. E; Juri,H; Femopase,G. A; Hidalgo,M; Ferrero,J. C; Rinaldi,C; Fonseca,I; Juri,G.
The Laser used correctly in the medical practice offers clear advantages compared with traditional therapies. The improvement and even the elimination of many significant skin lesions can be achieved with reduced risks to patients. However, it is important to keep security measures and understand the possible effects on an experimental model. The chick embryo is a good model to evaluate the direct effects of non-ionizing radiation for its easy handling and availability. The purpose of this communication is to show our histological findings in organs of the chick embryo with and without protective barrier to be subjected to radiation excimer. We used the following issuers: intense pulsed light (excimer Xe-Cl laser of 308 nm wavelength). It was irradiated...
Tipo: Journal article Palavras-chave: Biological effects; Intense pulsed light (Xe-Cl); Cartilage; Tongue; Chick embryo.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000400007
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In vitro Study of Morphological Changes of the Cultured Otocyst Isolated from the Chick Embryo International Journal of Morphology
Intarapat,Sittipon; Gonmanee,Thanasup; Thonabulsombat,Charoensri.
The aim of this study was to observe morphological changes of the cultured otocysts isolated from various stages of the chick embryo. Isolated otocysts were dissected from embryonic day, E2.5-4.5 of incubation (HH stage 16-26) according to stages of developing inner ear. Morphology of the chick otocyst exhibited an ovoid shape. The width and height of the otocyst were 0.2 mm and 0.3 mm, respectively. Elongation of a tube-like structure, the endolymphatic duct, was found at the dorsal aspect of the otocyst. The cultured otocyst is lined by the otic epithelium and surrounding periotic mesenchymal cells started to migrate outwards the lateral aspect of such epithelium. Notably, the acoustic-vestibular ganglion (AVG) was observed at the ventrolateral aspect of...
Tipo: Journal article Palavras-chave: Chick embryo; Inner ear; Otocyst; Otic development.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100034
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