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| NIU,Rui; YANG,Yamei; WANG,Yadan; LUO,Shilong; ZHANG,Cheng; WANG,Yun. |
| Abstract In this study, an immunoaffinity column was prepared by covalently coupling of polyclonal antibody against danofloxacin (DAN) with chitosan microparticles and its potential application to selective extraction of DAN from food samples was also evaluated. The produced antibody exhibited high sensitivity, with an IC50 value of 4.56 ng/mL towards DAN, and good specificity, with less than 0.5% of cross-reactivity towards DAN structurally related compounds. The developed immunoaffinity column had an approximate binding capacity of 2300 ng/mL gel and a relative standard deviation (RSD) of 3.7% among columns. The immunoaffinity columns were then challenged with DAN-fortified milk samples, recoveries of DAN were found to be in the range of 83.8% to 98.8%,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Danofloxacin; Immunoaffinity chromatography; Clean-up; Residual detection. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000200500 |
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| Tolosa, Imma; Huertas, David; Choyke, Sarah; Sander, Sylvia; Aminot, Yann. |
| Two different sample preparation protocols for the determination of 37 emerging and historical halogenated flame retardants (HFRs) in marine tissues were compared with regards to extraction recovery, lipid removal efficiency, repeatability, reproducibility, and ability to measure sub-ng g−1 (dry weight) concentrations in marine biota. One method involved a purification step using gel permeation chromatography (GPC) followed by a HPLC fractionation step on a Partisil amino-cyano normal phase (GPC-Partisil procedure) and the other more traditional method was based on sulphuric acid treatment followed by silica column fractionation (H2SO4-silica procedure). The samples were analysed by gas chromatography (GC) and liquid chromatography (LC) tandem mass... |
| Tipo: Text |
Palavras-chave: Halogenated flame retardants; Biota; Clean-up; GC-MS/MS; LC-MS/MS; Emerging contaminants. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00654/76570/77700.pdf |
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