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	Provedor de dados BABT (1) Autor Gabriel,Jane Eire (1) Gonçalves,Dicezar (1) Madeira,Humberto Maciel França (1) Schühli,Guilherme Schnell e (1) Vicente,Vânia Aparecida (1) Palavra-chave Clinical mastitis (1) Mastitis detection (1) Molecular markers (1) Staphylococcus aureus (1) Subclinical mastitis (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2010 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última New method for early detection of two random amplified polymorphic DNA (RAPD) groups of Staphylococcus aureus causing bovine mastitis infection in Paraná State, Brazil BABT Gonçalves,Dicezar; Gabriel,Jane Eire; Madeira,Humberto Maciel França; Schühli,Guilherme Schnell e; Vicente,Vânia Aparecida. The aim of this work was to develop a fast and accurate molecular approach to allow early detection of two RAPD groups of S. aureus causing bovine mastitis. Seventy five S. aureus isolates from infected animals were characterized by RAPD. Genomic fragments isolated from the unique bands present in either group were cloned and sequenced. Based on the DNA sequences, specific primers were designed to allow for the simultaneous detection of either group by multiplex PCR of S. aureus DNA isolated from clinical and subclinical bovine mastitis. Results showed that these proposed primers set could be used to detect various clinical and subclinical S. aureus isolates as well as the detection of the microorganism in bulk milk. Their use as a specific method for... Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Molecular markers; Subclinical mastitis; Clinical mastitis; Mastitis detection. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200014 Registros recuperados: 1 Primeira ... 1 ... Última

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