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| Secchi,Carina; Antunes,Ana Lúcia Souza; Perez,Leandro Reus Rodrigues; Cantarelli,Vlademir Vicente; d'Azevedo,Pedro Alves. |
| The NCCLS (2004) presented a new methodology to detect, by disk-diffusion agar, oxacillin-resistance using a cefoxitin disk. We identified coagulase-negative staphylococci (SCoN) to the species level and compared the use of cefoxitin disks (30 µg) with oxacillin disks (1 µg), agar dilution (minimum inhibitory concentration of oxacillin) and mecA gene detection in isolates of coagulase-negative bacteria other than Staphylococcus epidermidis (SCoNne). A total of 238 SCoNne was evaluated; oxacillin-resistance (the mecA gene) was detected in 71% of the isolates. All methods gave 100% sensitivity, based on presence of the mecA gene. The specificity of the cefoxitin disk was 100%, while the oxacillin disk gave a specificity of 91% and agar dilution oxacillin... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coagulase-negative Staphylococcus; Cefoxitin; Oxacillin; MecA gene; Susceptibility diagnostic. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400012 |
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| Mantovani,R. P.; Rall,V. L. M.; Batalha,J. E. N.; Fernandes,A. A. H.; Fernandes Junior,A.. |
| Propolis is a natural resinous substance collected by bees from vegetal sources and its therapeutic properties have been investigated. In this work, we evaluated the inhibitory activity of ethanolic extracts of propolis (EEP) from the Southeast and South of Brazil on coagulase-negative Staphylococcus (CNS) growth as well as the EEP in vitro synergism with antimicrobial drugs by using the diffusion method (E-test). The EEP chemical characteristics (dry weight, pH, flavonoid and phenolic compounds) were determined. Seven drugs were tested, and synergism was observed between three drugs and Southeast EEP, six drugs and South EEP, and one drug and ethanol control. Ethanolic extracts of propolis from the South of Brazil presented the greatest flavonoid content... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brazilian propolis; Coagulase-negative Staphylococcus; E-test; Antimicrobial drugs; Synergism. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200012 |
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