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	Provedor de dados Biocell (1) BABT (1) BJMBR (1) BJM (1) Autor Berend,K.R. (1) Brizuela,Nilda Y. (1) Demurtas,Silvia L. (1) Duarte Neto,José Manoel Wanderley (1) Harrelson,J.M. (1) Krishnamoorthy,Kannan (1) Landge,Anil Vijay (1) Lima Filho,José Luiz de (1) Lima,Carolina de Albuquerque (1) Meirovich,Carlos I. (1) Montrull,Hilda L. (1) Porto,Ana Lúcia Figueiredo (1) Qi,W.-N. (1) Scully,S.P. (1) Spitale,Luis (1) Teixeira,José António Couto (1) Wanderley,Maria Carolina de Albuquerque (1) Mais... Palavra-chave Collagenase (4) Characterization (1) Chondrocytes (1) Chondrosarcoma (1) Clostridium novyi-NT (1) Fibro-proliferative disorders (1) Fungus (1) Gene expression (1) Metastasis (1) Osteoarthritis (1) Production (1) Prognosis (1) Purification (1) Response Surface Methodology (1) Mais... Tipo do documento Info:eu-repo/semantics/article (4) Ano 2018 (1) 2017 (1) 2005 (1) 1999 (1) País Brazil (3) Argentina (1) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Structure and secretory activity of cultured chondrocytes from patients with osteoarthritis Biocell Montrull,Hilda L.; Brizuela,Nilda Y.; Demurtas,Silvia L.; Spitale,Luis; Meirovich,Carlos I.. Cartilage samples were taken from OA patients in order to describe and quantify pro-inflammatory mediators. Samples were cultured under aseptic conditions in Dulbecco's modified Eagle medium at 37°C for 10 days. Control samples, taken from non-inflammatory cartilage, were cultured under the same conditions. The levels of NO-2 and NO-3 were measured in the supernatant using a spectrophotometric assay. The activity of MMP-1 was quantified by ELISA. The concentration of NO-x was 47.3 ± 4.1 µM in the OA cartilague and 10.7 ± 1.8 µM in the controls. The average MMP-1 activity was 3,650 ± 387 ng/ml in the OA cartilage and 2,150 ± 190 ng/ml in the control samples. These increased values of MMP-1 and NO-x observed in the OA cartilage suggest a higher catabolic... Tipo: Info:eu-repo/semantics/article Palavras-chave: Osteoarthritis; Chondrocytes; Collagenase. Ano: 2005 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200002 Statistical Optimisation of Fermentation Media for Novel Clostridium novyi -NT Using Response Surface Methodology. BABT Krishnamoorthy,Kannan; Landge,Anil Vijay. ABSTRACT Clostridial collagenase is recognized as one of the important proteolytic enzymes used in treatment of varieties of fibro-proliferative disorders. The present work aimed to optimise the biomass concentration and collagenase enzyme activity of novel bacilli Clostridium novyi-NT. The response surface methodology tool was used to identify the optimal fermentation parameters. Central composite design (CCD) was applied with respect to three influencing factors - pH, proteose peptone and trypticase soya broth. These factors showed significant effect on the overall biomass concentration and collagenase enzyme activity (p<0.05). The maximum biomass concentration in terms of absorbance and collagenase activity (of crude enzyme) was achieved and recorded... Tipo: Info:eu-repo/semantics/article Palavras-chave: Collagenase; Clostridium novyi-NT; Fibro-proliferative disorders; Response Surface Methodology. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100429 Collagenase specificity in chondrosarcoma metastasis BJMBR Scully,S.P.; Berend,K.R.; Qi,W.-N.; Harrelson,J.M.. The treatment of some mesenchymal malignancies has made significant gains over the past few decades with the development of effective systemic therapies. In contrast, the treatment of chondrosarcoma has been limited to surgical resection, with the most significant prognostic indicators being surgical margins and histologic grade. We have reported that MMP-1/TIMP-1 gene expression serves to prognosticate for tumor recurrence in this group of patients. This led to the hypothesis that collagenase activity facilitates cell egression from the cartilaginous matrix. In the current study we examine the specificity of collagenase gene expression in archival human chondrosarcoma samples using semi-quantitative PCR. Messenger RNA was affinity extracted and subject to... Tipo: Info:eu-repo/semantics/article Palavras-chave: Chondrosarcoma; Metastasis; Collagenase; Gene expression; Prognosis. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700013 Collagenolytic enzymes produced by fungi: a systematic review BJM Wanderley,Maria Carolina de Albuquerque; Duarte Neto,José Manoel Wanderley; Lima Filho,José Luiz de; Lima,Carolina de Albuquerque; Teixeira,José António Couto; Porto,Ana Lúcia Figueiredo. Abstract Specific proteases capable of degrading native triple helical or denatured collagen have been required for many years and have a large spectrum of applications. There are few complete reports that fully uncover production, characterization and purification of fungi collagenases. In this review, authors searched through four scientific on line data bases using the following keywords (collagenolytic OR collagenase) AND (fungi OR fungus OR fungal) AND (production OR synthesis OR synthesize) AND (characterization). Scientific criteria were adopted in this review to classify found articles by score (from 0 to 10). After exclusion criteria, 21 articles were selected. None obtained the maximum of 10 points defined by the methodology, which indicates a... Tipo: Info:eu-repo/semantics/article Palavras-chave: Collagenase; Fungus; Characterization; Purification; Production. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100013 Registros recuperados: 4 Primeira ... 1 ... Última

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