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Differential gene expression, induced by salicylic acid and Fusarium oxysporum f. sp. lycopersici infection, in tomato PAB
Amaral,Daniel Oliveira Jordão do; Lima,Marleide Magalhães de Andrade; Resende,Luciane Vilela; Silva,Márcia Vanusa da.
The objective of this work was to determine the transcript profile of tomato plants (Lycopersicon esculentum Mill.), during Fusarium oxysporum f. sp. lycopersici infection and after foliar application of salicylic acid. The suppression subtractive hybridization (SSH) technique was used to generate a cDNA library enriched for transcripts differentially expressed. A total of 307 clones was identified in two subtractive libraries, which allowed the isolation of several defense-related genes that play roles in different mechanisms of plant resistance to phytopathogens. Genes with unknown roles were also isolated from the two libraries, which indicates the possibility of identifying new genes not yet reported in studies of stress/defense response. The SSH...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lycopersicon esculentum; CDNA libraries; Complementary DNA; Gene expression; Salicylic acids; Suppression subtractive hybridization.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000800010
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