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Genotoxic evaluation of sodium nitroprusside in Aspergillus nidulans Genet. Mol. Biol.
Chiuchetta,Simone Jurema Ruggeri; Castro-Prado,Marialba Avezum Alves de.
The exogenous nitric oxide donor, sodium nitroprusside, evaluated the recombinogenic potential of nitric oxide. Drug inhibited mycelial growth and conidiation in A757 Aspergillus nidulans master strain. Two heterozygous diploid strains, one wild (uvsH+//uvsH+) and the other defective to DNA repair (uvsH//uvsH) were used for recombinagenesis tests. Sodium nitroprusside recombinogenic effect was evaluated by the induction of homozygosis of recessive genes, originally present in heterozygous condition. Results show that sodium nitroprusside (40 muM, 80 muM and 160 muM) is effective in inducing mitotic crossing-over in diploid cells of A. nidulans.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nitric oxide; Parasexual cycle; Homozygotization index; Conidiation; Mitotic recombination.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500023
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Compatibility of conventional agrochemicals used in rice crops with the entomopathogenic fungus Metarhizium anisopliae Scientia Agricola
Silva,Rodrigo Alves da; Quintela,Eliane Dias; Mascarin,Gabriel Moura; Barrigossi,José Alexandre Freitas; Lião,Luciano Moraes.
The toxicological impact of chemical pesticides on fungal entomopathogens and their use in tank-mixing can be directly measured through in vitro compatibility tests. This study reports the in vitro toxicity of eight insecticides, four fungicides and five herbicides in the conidial germination, vegetative growth and conidiation of Metarhizium anisopliae (strain CG 168). A conidial suspension containing the pesticide at recommended field dosage was subjected to constant agitation in a rotary shaker for 3h to simulate a tank mixing. Then, aliquots of each suspension were used to determine conidial germination, vegetative growth and conidiation on potato dextrose agar (PDA). The fungicides difenoconazole (69 mL ha-1), propiconazole (75 mL ha-1),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pesticide compatibility; Conidiation; Entomopathogenic fungi; Germination; Vegetative growth.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162013000300003
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Genetic and cytological characterization of a developmental mutant of Aspergillus nidulans induced by 5-azacytidine Biol. Res.
COSTA,MARCO ANTONIO; SILVA,NELMARA CRISTINA GONÇALVES; CASTRO-PRADO,MARIALBA AVEZUM ALVES.
An analysis of a new medusa mutant of Aspergillus nidulans obtained by 5-azacytidine-treatment and named B116 is provided. The B116 mutant was phenotypically characterized by the production of conidiophores with reduced pigmentation and vesicles bearing multiple tiers of sterigmata. A single nuclear gene located on chromosome I is responsible for phenotypical changes in the mutant. The 5-azacytidine-altered locus, designated medA102, is recessive in heterozygous diploid and the medusa mutant is a Dp(II,I) duplication bearer that renders the strain mitotically unstable.
Tipo: Journal article Palavras-chave: Conidiation; Developmental program; Medusa.
Ano: 2001 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200012
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