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| Registros recuperados: 38 | |
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| Sartori,L.C.A.; Santos,R.C.; Marin,J.M.. |
| A mastite representa um dos problemas mais sérios que afetam as fazendas de gado leiteiro. As mastites clínica e subclínica em bovinos, devido a leveduras, são causadas principalmente por espécies do gênero Candida. O objetivo deste trabalho foi identificar quais espécies dessa levedura estavam presentes em amostras de leite obtidas da glândula mamária de vacas apresentando mastite. Um total de 428 amostras de leite foi coletado nos estados de São Paulo, Paraná, Santa Catarina e Rio Grande do Sul. A identificação foi realizada por meio de critérios fenotípicos e fisiológicos. Foi possível isolar seis espécies de Candida em 55 (12,8%) das amostras. As espécies mais frequentes foram C. krusei(34,6%), C. parapsilosis(25,4%), C. tropicalis (18,2%) e C.... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Cow; Bovine mastitis; Milk; Yeast; Candida. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000501615 |
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| Resende,D.; Santo,E.; Macedo,C.; Marin,J.M.. |
| The prevalence of virulence genes expressing fimbriae, production of hemolysin, colicin and aerobactin, was determined in Escherichia coli isolates from healthy cows genital tract not showing clinical signs of infection. The presence of fimbriae expression genes (pap, sfa, afa) was assayed using specific primers in a polymerase chain reaction; none were detected in any of the isolates. Yet, a prevalence of 90.4%, 69.8%, and 28.5% of virulence factors for colicin, hemolysin and aerobactin respectively, was detected in the isolates. Analysis of the bacterial pathogenicity of isolates from the bovine genital tract may contribute towards the understanding of E. coli behavior. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cow; Escherichia coli; Genital tract; Virulence factors. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000300003 |
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| Pessoa,G.A; Navarro,R.B; Rauber,L.P; Brass,K.E; Silva,C.A.M; Rubin,M.I.B. |
| Temperature and rainfall were analyzed daily during six years to evaluate their influence on in vitro production of bovine embryos. Weekly replications (n=480) were performed on 14,778 ovaries collected at slaughterhouses. Cumulus oocyte complexes (n=19,180) were fertilized with a pool of Bos taurus taurus semen in one incubator with 5% CO2. Presumable zygotes were cultured in gasified plastic bags with 5% CO2, 5% O2, and 90% N2. In the first year, cleavage and embryo yield were 60.3% and 15.6%, respectively, being lower (P<0.05) than in the following years. Average cleavage rates were always lower in winter (P<0.0001), thus producing less embryos. Winter climatic conditions had a negative influence on in vitro production, when cleavage and embryo... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cow; Oocytes; Embryo; Climate; In vitro prodution. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600013 |
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| Barbosa,Antônio Amaral; Araújo,Maria Carolina Narval de; Krusser,Rafael Herbstrith; Martins,Charles Ferreira; Schmitt,Eduardo; Rabassa,Viviane Rohrig; Pino,Francisco Augusto Burkert Del; Brauner,Cássio Cassal; Corrêa,Marcio Nunes. |
| ABSTRACT: The aim of this study was to confirm the possible consequences of prepartum lameness on subsequent lactation among Holstein cows. In this research, 27 multiparous cows, were monitored from the 30th to 63rd day, relative to calving. Thirty days prior to parturition, the animals were segregated into two groups based on the locomotion score (LS), where LS 1 is attributed to animals without claudication, LS 2 to those with suspected lameness and LS 3, 4 and 5 to those with mild, moderate and severe lameness, respectively; in the lame cows group (LC) (n=15), the animals displayed LS ≥ 3, whereas in the non-lame cows group (NLC) (n=12) they showed LS 1. Milk production, body condition score (BCS) and blood concentration levels of β-hydroxybutyrate... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lameness; Cow; Transition period. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000500701 |
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| ROSA, A. do N.; MELO, J. de. |
| O Pantanal Mato-Grossense encontra-se na bacia do Rio Paraguai, estendeu-se por cerca de 250 km na direcao leste-oeste e 450 km na direcao norte-sul, perfazendo uma area total de 140.000 km2. A planicie pantaneira apresenta 80 a 200 m de altitude, sendo circundada por um planalto, com 300 a 700 m, que contribui para a planicie com agua e sedimentos. O clima e quente e chuvoso no verao e ameno e seco no inverno, com temperaturas medias de 32oC e 21oC, respectivamente. A precipitacao pluviometrica anual esta entre 1000 e 1400 mm, concentrada entre os meses de dezembro a marco. Cerca de 92% da planicie pantaneira e constituida por solos hidromorficos, sendo a maioria (70%) de baixa fertilidade natural. Em todas as sub-regioes, a estrutura fundiaria... |
| Tipo: Folhetos |
Palavras-chave: Bovine; Cow; Production; Bovino; Produção; Touro; Pantanal; Cattle. |
| Ano: 1995 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/784396 |
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| 山川, 和宏; 杉崎, 義一; 吉林台; 古林, 与志安; 古岡, 秀文; 佐々木, 直樹; 石井, 三都夫; 猪熊, 壽; YAMAKAWA, Kazuhiro; SUGISAKI, Giichi; Jilintai; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi; SASAKI, Naoki; ISHII, Mitsuo; INOKUMA, Hisashi. |
| 2歳5カ月齢ホルスタイン雌乳牛が胸垂冷性浮腫，頸静脈拍動を呈し，心音聴取困難および心電図各波低電位所見から拡張型心筋症が疑われた．右胸腔には赤色変性性漏出液が多量に貯留しており，また超音波検査により心嚢水貯留を認めた．病理解剖の結果，心嚢は直径60cm大に腫大して血様心嚢水の貯留を認めたが，炎症あるいは腫瘍は認められず，血様心嚢水貯留の原因については不明であった．ただし右肩部に壊死巣を認めたことから，転倒等胸腔に強い衝撃を受けた可能性があると考えられた．心嚢水貯留が認められる場合には，心嚢腔内への出血も考慮すべき疾病のひとつであることが考えられた． A Holstein cow (two years and five months old) with jugular vein distension and non-inflammatory edema in the brisket showed decreased heart sound and abnormal waves on an electrocardiogram. Dilated cardiomyopathy was suspected. Unilateral pleural effusion was recovered from the right pleural cavity and found to be degenerated transudations. An ultrasonic cardiogram detected pericardial fluid. A pathological examination revealed that the pericardial cavity was enlarged with a... |
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Palavras-chave: 牛; 血様心嚢水; 片側性胸水貯留; Cow; Blood-like pericardium; Unilateral pleural efftusion. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2804 |
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| HAYASHI, Ken-Go; MATSUI, Motozumi; ACOSTA, Tomas J.; KIDA, Katsuya; MIYAMOTO, Akio; 松井, 基純; 木田, 克弥; 宮本, 明夫. |
| Luteinizing hormone (LH) surge and follicle rupture act as trigger to start corpus luteum (CL) formation. Thus, we aimed to investigate whether a dominant follicle that has not been exposed to an LH surge can become a functional CL. For this purpose, follicular fluid from the dominant follicles (DF) of cows was aspirated before or after a GnRH-induced LH surge, and subsequent CL formation was observed. Holstein cows were divided into four groups as follows: Luteal phase, a DF was aspirated 7 days after GnRH injection; Pre-LH surge, a DF was aspirated 42 h after PGF2α injection during the mid luteal phase; Post-LH surge, a DF was aspirated 24 h after GnRH injection following PGF2α; and Intact follicle, ovulation was induced by GnRH injection after PGF2α.... |
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Palavras-chave: Corpus luteum formation; Cow; Follicle aspiration; LH surge. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2982 |
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| 猪熊, 壽; 滄木, 孝弘; 山川, 和宏; 竹内, 俊彦; 松本, 高太郎; 石井, 三都夫; 古林, 与志安; 古岡, 秀文; INOKUMA, Hisashi; AOKI, Takahiro; YAMAKAWA, Kazuhiro; TAKEUCHI, Toshihiko; MATSUMOTO, Kotaro; ISHII, Mitsuo; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi. |
| 病理学的に血様液貯留を特徴とする特発性心嚢水腫と診断された乳牛8症例（血様心嚢水貯留群）の臨床病理学的検査所見を回顧的に取りまとめ，心膜炎7症例（心膜炎群）の所見と比較した．血様心嚢水貯留群では冷性浮腫を呈する症例が心膜炎群に比べて多かったが，その他の臨床症状には両群で差がなかった．心エコー検査では血様心嚢水貯留群では，心膜炎でみられる心嚢内の顕著なフィブリン析出はなかった．血液及び生化学検査では，血様心嚢水貯留群では炎症像が弱く，心膜炎群と比較して白血球数，分葉核好中球割合，A/G比，アルブミン濃度，γグロブリン割合に有意差がみられた．両群でALP及びGGTの高値がみられたが，特に血様心嚢水貯留群のGGT増加は顕著であった．血様心嚢水貯留症例の診断は臨床症状だけでは困難であるが，心エコー検査所見，弱い炎症像，ALP及びGGTの高値などが心膜炎との鑑別診断に有用と考えられた． Clinicopathological findings of eight cases of idiopathic hydropericardium characterized by bloodhlike fluid (HBFhgroup) that were confirmed by necropsy were retrospectively summarized and compared with those of seven cows with pericarditis. Although the numbers of cows that showed edema in the HBFhgroup were larger than those in the pericarditishgroup, the frequency of... |
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Palavras-chave: 特発性心餒水腫; 心膜炎; 血様心嚢水; 牛; Idiopathic hydropericardium; Pericarditis; Blood-like pericardium; Cow. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3557 |
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| ACOSTA, Tomas J.; HAYASHI, Ken-Go; MATSUI, Motozumi; MIYAMOTO, Akio; 松井, 基純; 宮本, 明夫. |
| Increase in the blood supply to individual follicles appears to be associated with follicular growth rates and the ability to become the dominant follicle, while reduced thecal vascularity appears to be closely associated with follicular atresia. Therefore, this study aimed to determine the real-time changes in the vascularity of the follicle wall during the first follicular wave in cycling Holstein cows. Normally cycling and lactating cows (n=5) were examined by transrectal color Doppler ultrasonography (the sensitivity for velocity: > 2 mm/sec) to determine the changes in the vasculature of the follicle wall (presence or absence of blood flow) and the diameter of follicles. A new follicular wave and ovulation were induced by GnRH injection at 48 h... |
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Palavras-chave: Blood flow; Follicular wave; Selection; Deviation; Cow. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2979 |
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| Ozawa, Takahiro; Acosta, Tomas J.; Nakamura, Tadashi; Miyamoto, Akio; 中村, 正; 宮本, 明夫. |
| The presence of gonadotropin-releasing hormone (GnRH)-like protein has been previously demonstrated in the bovine follicle. This GnRH-like protein was purified and concluded to be histone H2A. However, neither GnRH peptide nor specific GnRH-immunoreactivity (GnRH-IR) has been demonstrated in the bovine follicle so far. Thus, this study focused on the detection of specific GnRH-IR using the second-antibody enzyme immunoassay in isolated bovine mature follicles, and on an examination of the direct effect of luteinizing hormone (LH), endothelin-1 (ET-1), and cytokines on the GnRH-IR using an in vitro microdialysis system (MDS). We further examined a cross-reactivity of the GnRH antibody with bovine histone H2A. GnRH-IR was detected in microdialyzed perfusate... |
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Palavras-chave: GnRH; Immunoreactivity; Microdialysis; Follicle; Cow. |
| Ano: 2000 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2970 |
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| HAYASHI, Ken-Go; BERISHA, Bajram; MATSUI, Motozumi; SCHAMS, Dieter; MIYAMOTO, Akio; 松井, 基純; 宮本, 明夫. |
| Recent findings indicate that the changing profile of angiopoietins (ANPT) and their receptor Tie2 are closely associated with development and regression of the vascular network in the cyclic ovary. We previously reported that mRNA expression for the ANPT-Tie system in theca interna changes during bovine follicular development and atresia, and both ANPTs affect steroidogenesis in the preovulatory follicle. The aim of this study was to investigate mRNA expression for ANPT1, ANPT-2 and Tie2 in granulosa cells (GC) during follicular development in the cow. Bovine follicles were classified according to the estradiol-17β (E2) concentration in follicular fluid (FF) as follows: (1) E2<0.5, (2) 0.5<E2<5, (3) 5<E2<20, (4) 20<E2<180 and (5)... |
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Palavras-chave: Follicular development; Angiopoietin-Tie system; Granulosa cell; Cow. |
| Ano: 2004 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2975 |
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| Watanabe, Sho; Shirasuna, Koumei; Matsui, Motozumi; Yamamoto, Dai; Berisha, Bajram; Schams, Dieter; Miyamoto, Akio; 白砂, 孔明; 松井, 基純; 宮本, 明夫. |
| Endothelin-1 (ET-1) is a luteolytic mediator in the bovine corpus luteum (CL), and its action appears to be via endothelin type A receptor (ETR-A). Thus, the aim of the present study was to determine the effect of ETR-A antagonist on PGF(2)alpha-induced luteolysis in the cow. Cows on days 1012 of the estrous cycle were subjected to five intraluteal injections of the ETR-A antagonist LU 135252 in saline or only saline at -0.5, 2, 4, 6, and 8 h after PGF(2)alpha administration (=0 h). Serial luteal biopsies were conducted to determine the expression of mRNA in the luteal tissue. There were no significant differences in the decrease in plasma progesterone (P) concentrations and the mRNA expressions of steroidogenic acute regulatory protein and 3... |
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Palavras-chave: Apoptosis; Blood flow; Corpus luteum; Cow; Endothelin-1. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/922 |
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| MIURA, Ryotaro; HANEDA, Shingo; MATSUI, Motozumi; 羽田, 真悟; 松井, 基純. |
| The objective of our study was to compare the characteristics of the corpus luteum (CL) formed after ovulation of the dominant follicle (DF) of the first follicular wave (W1) and those of the CL formed after ovulation of the DF of the second (induced) follicular wave (W2). Non-lactating Holstein cows were used for this study. In Experiment 1, cows were treated with PGF2α and GnRH on days 6 and 8 (day 0 = day of follicular wave emergence) for W1 (n = 6) and W2 (n = 6), respectively. Dominant follicles were aspirated on day 9 to quantify the amounts of mRNA (VEGF120, VEGF164, FGF-2, StAR, P450-scc and 3β-HSD) in granulosa cells (GC). In Experiment 2, the size and blood flow area of the CL formed after ovulation of the DF in W1 (W1CL; n = 6) and W2 (W2CL; n =... |
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Palavras-chave: Blood flow; Corpus luteum; Cow; Follicular wave; Gene expression. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4147 |
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| Registros recuperados: 38 | |
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