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Isolation, morphological and molecular characterization of Bacillus thuringiensis strains against Hypothenemus hampei Ferrari (Coleoptera: Curculionidae: Scolytinae) Rev. Bras. entomol.
Zorzetti,Janaina; Ricietto,Ana Paula Scaramal; Fazion,Fernanda Aparecida Pires; Meneghin,Ana Maria; Neves,Pedro Manuel Oliveira Janeiro; Vilas-Boas,Laurival Antonio; Vilas-Bôas,Gislayne Trindade.
ABSTRACT The coffee berry borer Hypothenemus hampei Ferrari, 1876 (Coleoptera: Curculionidae: Scolytinae) is considered the most serious pest of the coffee crop and is controlled primarily with the use of chemical insecticides. An alternative to this control method is the use of the entomopathogenic bacterium, Bacillus thuringiensis Berliner, 1911. Therefore, the objective of this work was to select strains of B. thuringiensis virulent against H. hampei and characterize them by morphological and molecular methods to identify possible genes for the production of genetically modified plants. To achieve this objective, 34 strains of B. thuringiensis underwent a selective bioassay to evaluate their toxicity to H. hampei first-instar larvae. Among the strains...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological control; Entomopathogenic bacteria; Coffee berry borer; Cry proteins.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262018000300198
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Characterization of the mucosal and systemic immune response induced by Cry1Ac protein from Bacillus thuringiensis HD 73 in mice BJMBR
Vázquez-Padrón,R.I.; Moreno-Fierros,L.; Neri-Bazán,L.; Martínez-Gil,A.F.; de-la-Riva,G.A.; López-Revilla,R..
The present paper describes important features of the immune response induced by the Cry1Ac protein from Bacillus thuringiensis in mice. The kinetics of induction of serum and mucosal antibodies showed an immediate production of anti-Cry1Ac IgM and IgG antibodies in serum after the first immunization with the protoxin by either the intraperitoneal or intragastric route. The antibody fraction in serum and intestinal fluids consisted mainly of IgG1. In addition, plasma cells producing anti-Cry1Ac IgG antibodies in Peyer's patches were observed using the solid-phase enzyme-linked immunospot (ELISPOT). Cry1Ac toxin administration induced a strong immune response in serum but in the small intestinal fluids only anti-Cry1Ac IgA antibodies were detected. The data...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cry proteins; Bacillus thuringiensis; Mucosal immunology.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200002
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Bacillus thuringiensis monogenic strains: screening and interactions with insecticides used against rice pests BJM
Pinto,Laura M.N.; Dörr,Natália C.; Ribeiro,Ana Paula A.; Salles,Silvia M. de; Oliveira,Jaime V. de; Menezes,Valmir G.; Fiuza,Lidia M..
The screening of Bacillus thuringiensis (Bt) Cry proteins with high potential to control insect pests has been the goal of numerous research groups. In this study, we evaluated six monogenic Bt strains (Bt dendrolimus HD-37, Bt kurstaki HD-1, Bt kurstaki HD-73, Bt thuringiensis 4412, Bt kurstaki NRD-12 and Bt entomocidus 60.5, which codify the cry1Aa, cry1Ab, cry1Ac, cry1Ba, cry1C, cry2A genes respectively) as potential insecticides for the most important insect pests of irrigated rice: Spodoptera frugiperda, Diatraea saccharalis, Oryzophagus oryzae, Oebalus poecilus and Tibraca limbativentris. We also analyzed their compatibility with chemical insecticides (thiamethoxam, labdacyhalothrin, malathion and fipronil), which are extensively used in rice crops....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus thuringiensis; Cry proteins; Insects.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200025
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In vitro effect of Bacillus thuringiensis strains and Cry proteins in phytopathogenic fungi of paddy rice-field BJM
Knaak,Neiva; Rohr,Angelise Ana; Fiuza,Lidia Mariana.
Cry1Ab and Cry1Ac strains and proteins synthesized by Bacillus thuringiensis thuringiensis and B. thuringiensis kurstaki were assessed in the following phytopathogens: Rhizoctonia solani,Pyricularia grisea,Fusarium oxysporum and F. solani, which had their micelial growth decreased after incubation in the presence of the bacterial strains. As to Cry proteins, there were no inhibition halo development in the assessed concentrations.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Paddy rice; Bacillus thuringiensis; Phytopathogens; Cry proteins.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300027
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