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Cryopreservation of banana’s cv Grand Naine in vitro rhizomes Anais da ABC (AABC)
LONDE,LUCIANA C.N.; VENDRAME,WAGNER A.; SANAEI,MASSY; OLIVEIRA,ALEXANDRE B. DE.
ABSTRACT The preservation of banana genetic material is usually performed through seedlings. However, most banana cultivars do not produce seed and are propagated vegetatively. Therefore, cryopreservation is a feasible technique that allows the preservation of banana genotypes indefinitely. For the success of cryopreservation protocols, the selection of cryoprotectants and pre-freezing techniques are important factor. Therefore, the objective of this study was to verify the effects of different cryoprotectants with and without 1% phloroglucinol and pre-cooling periods on the development of a protocol for cryopreservation of in vitro rhizomes ofMusa accuminata(AAA) cv Grand Naine banana. The addition of 1% phloroglucinol to the cryoprotective solutions,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Musa acumminata; In vitro conservation; Cryoprotectants; Pre-cooling.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100495
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In vitro viability of canine spermatozoa frozen in tris-fructose-citric acid extender with ethylene glycol Braz. J. Vet. Res. Anim. Sci.
VANNUCCHI,Camila Infantosi; SANTOS,Silvia Edelweiss Crusco dos; VISINTIN,José Antonio.
The use of frozen semen has become increasingly popular in canine artificial insemination, stimulating research to improve freezing methods and extenders. In this study, was compared the final concentration of 5% ethylene glycol used in 3 equilibration protocols. In Method I, one part of semen was added to two parts of tris-fructose-citric acid with 7.5% ethylene glycol (Extender 2) and frozen without cooling. In Method II, one part of semen was added to two parts of tris-fructose-citric acid with 7.5% ethylene glycol (Extender 2) and cooled at 5ºC for 1 hour before freezing. In Method III, one part of semen was added to one part of tris-fructose-citric without ethylene glycol (Extender 1) and cooled at 5ºC for 1 hour. Just after this period, one part of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ethylene glycol; Cryoprotectants; Freezing; Semen; Dog.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961999000400007
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Cryopreservation of testicular tissue: an alternative to maintain the reproductive capacity in different animal species Ciência Rural
Lima,David Baruc Cruvinel; Silva,Lúcia Daniel Machado da.
ABSTRACT: Cryopreservation of testicular tissue enables the maintenance of reproductive capacity in different animal species, and contributes to the formation of gene banks for endangered species. The spermatogonia present in the testes can be grown in vitro and the sperm obtained can be used in artificial breeding programs. This review aimed to describe the main techniques of testicular cryopreservation, the main cryoprotectants used, as well as the progress made in different animal species thus far. In the last decade, significant progress has been made in obtaining viable and functional germ cells from testicular tissue. However, more research is needed to better establish protocols that can be used in clinical practice with various species.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Testicle; Conservation; Cryoprotectants.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001100701
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