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Genetic diversity analysis of traditional and improved cultivars of Pakistani rice (Oryza sativa L.) using RAPD markers Electron. J. Biotechnol.
Rabbani,Malik Ashiq; Pervaiz,Zahida Hassan; Masood,Muhammad Shahid.
The molecular marker is a useful tool for assessing genetic variations and resolving cultivar identities. Information on genetic diversity and relationships among rice genotypes from Pakistan is currently very limited. The objective of this study was to evaluate the genetic polymorphisms and identities of 10 traditional, 28 improved and 2 Japanese cultivars of rice using the random amplified polymorphic DNA technique. Twenty-five decamer-primers could generate a total of 208 RAPD fragments, of which 186 or 89.4% were polymorphic. The number of amplification products produced by each primer varied from 4 to 16 with an average of 8.3 bands primer-1. The size of amplified fragments were ranged from 200 to 4000 bp. Pair-wise Nei and Li's similarity had...
Tipo: Journal article Palavras-chave: Cultivar discrimination; Genetic variability; Oryza sativa; Pakistani rice; RAPDs.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300006
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Number of replicates and experimental precision statistics in corn PAB
Cargnelutti Filho,Alberto; Toebe,Marcos; Alves,Bruna Mendonça; Kleinpaul,Jéssica Andiara; Neu,Ismael Mario Marcio.
Abstract: The objective of this work was to evaluate the influence of the number of replicates on experimental precision in corn (Zea mays). Data on the grain yield of 15 corn cultivars, evaluated in a randomized complete block design, with nine replicates (r = 9), were used. From the experiment with nine replicates, further experiments were conducted with combinations of 2, 3, ..., r-1 replicates. The analysis of variance was carried out for each experiment. Experimental precision statistics and central tendency and variability measures were calculated, dispersion diagrams were prepared, and models of these statistics were adjusted as a function of the number of replicates. The number of replicates was determined by the visual analysis of the graphs with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zea mays; Cultivar discrimination; Fasoulas’ index; Least significant difference.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018001101213
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Optimal use of SSR markers for varietal identification of upland cotton PAB
Pereira,Guilherme da Silva; Cazé,Ana Luíza Ramos; Silva,Michelle Garcia da; Almeida,Vanessa Cavalcante; Magalhães,Fernanda Oliveira da Cunha; Silva Filho,João Luís da; Barroso,Paulo Augusto Vianna; Hoffmann,Lúcia Vieira.
Abstract: The objective of this work was to identify polymorphic simple sequence repeat (SSR) markers for varietal identification of cotton and evaluation of the genetic distance among the varieties. Initially, 92 SSR markers were genotyped in 20 Brazilian cotton cultivars. Of this total, 38 loci were polymorphic, two of which were amplified by one primer pair; the mean number of alleles per locus was 2.2. The values of polymorphic information content (PIC) and discrimination power (DP) were, on average, 0.374 and 0.433, respectively. The mean genetic distance was 0.397 (minimum of 0.092 and maximum of 0.641). A panel of 96 varieties originating from different regions of the world was assessed by 21 polymorphic loci derived from 17 selected primer pairs....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gossypium hirsutum; Cultivar discrimination; Intraspecific polymorphism; Microsatellite.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2015000700571
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