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| Registros recuperados: 82 | |
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| ROSALES LOAIZA,Néstor; VERA,Patricia; AIELLO-MAZZARRI,Cateryna; MORALES,Ever. |
| Nitrogen concentration is an essential parameter in cyanobacterial cultures to produce enriched biomass with biotechnological purposes. Growth and biochemical composition of Nostoc LAUN0015, Nostoc UAM206, Anabaena sp.1 and Anabaena sp.2 were compared at 0, 4.25, 8.5 and 17 mM NaNO3. Cultures under laboratory conditions were maintained for 30 days at a volume of 500 mL. Anabaena sp.1 yielded the highest value of dry mass of 0.26 ± 2.49 mg mL-1 at 8.5 mM NaNO3. For chlorophyll, phycocyanin and phycoerythrin, maximum values were achieved at 17 mM NaNO3 with 18.09 ± 1.74, 102.90 ± 6.73 and 53.47 ± 2.40 μg mL-1, respectively. Nostoc LAUN0015 produced its maximum value of protein 644.86 ± 19.77 μg mL-1, and 890 mg mL-1 of carbohydrates in the absence of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anabaena; Biochemical composition; Biomass; Culture; Nitrate; Nostoc. |
| Ano: 2016 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2016000200003 |
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| Pereira, Zefa Valdivina; UFGD; Mussury, Rosilda Mara; UFGD; Almeida, Aparecida Barbosa de; Secretaria de educação do Município de Ponta Porã - MS; Sangalli, Andréia; UFGD. |
| The academic interest regarding the knowledge that people in general have about plants and their uses has increased significantly. Therefore, aiming to understand it better, this present work studied parts of the plant used by residents from Jardim Aeroporto, in Ponta Porã, Mato Grosso do Sul State, to prepare and use these medicinal plants. This city shares borders with Pedro Juan Caballero (Paraguay), a place where many people use these herbs. The residents use different kinds of plants which are planted around their houses. The leaf was the most used part to prepare the medicines. The plants used by this community belong to 17 families, 28 genera and 30 species, and the women from 30-40 age group with junior high school degree who know the plants, their... |
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Palavras-chave: 2.00.00.00-6 Ciências Biológicas ethnopharmacology; Ethnobotany; Use; Culture 2.03.00.00-0 ethnopharmacology; Ethnobotany; Use; Culture. |
| Ano: 2009 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/3206 |
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| Silva,D.G.; Silva,D.R.; Silva,P.R.L.; Cícero,E.A.S.; Ferraz,A.L.J.; Lemos,M.V.F.; Fagliari,J.J.. |
| The efficiency of the Polymerase Chain Reaction (PCR) combined with selective enrichment broth was compared with the standard microbiological techniques for detection of Salmonella Dublin in fecal samples of 10 to 15-days-old Holstein calves, experimentally infected with 10(8) CFU of Salmonella Dublin. Seventy-six fecal samples were analyzed using PCR associated with selenite cystine (SC) and Muller-Kauffmann tetrathionate (TMK) broths and standard microbiological techniques. Regardless of the selective enrichment broth used, the standard microbiological techniques were significantly better than PCR in detection of positive samples of Salmonella Dublin. However, the simultaneous use of both techniques provided detection of a larger number of positive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Calves; Feces; Salmonella Dublin; PCR; Culture. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000300035 |
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| Lima,L.A.; Lima,C.D.L.C.; Carvalho,L.R.; Margutti-Pinto,M.E.B.. |
| The heteroantagonism between Enterobacter agglomerans, isolated from the gastrointestinal tract of American vulture Coragyps atratus, and Pseudomonas aeruginosa isolated from a hospital environment was evaluated. The slow (layer) and the wells (direct) techniques were tested, using agar and soy tryptone broth pH 7.3 at 37ºC. Through the slow method from 196 tests, inhibition growth halos, related heteroantagonism phenomenon observed in 118, corresponding to 60.2% positive results. Equivalent positive results were detected using wells (direct) methodology. The seven samples of E. agglomerans tested were capable of revealing heteroantagonism in the experimental conditions; antagonism reveled by the presence of a clear growth inhibition halo. The added 1%... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Heteroantagonism; Culture; Enterobacter agglomerans; Pseudomonas aeruginosa. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000300010 |
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| Wasielesky Jr.,Wilson; Bianchini,Adalto; Castaño Sanchez,Cecilia.; Poersch,Luis Henrique. |
| Studies were carried out to investigate the effect of temperature, salinity, ammonia, nitrite and nitrate on food consumption of pink shrimp Farfantepenaeus paulensis. Juveniles (0.2 - 0.4 g) were acclimated for 15 days in seawater with different temperatures, salinities and concentrations of ammonia, nitrite and nitrate. After the acclimation period, 20 shrimps per treatment were individualized in order to have their ration intake analyzed through the amount of ration offered and left over within a 24-hour period. Mean food consumption presented significant alterations (P<0.05) for the tested temperatures and nitrite concentrations, whereas for the salinity, ammonia and nitrate treatments, shrimp presented no alteration on food intake (P>0.05).... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Culture; Food consumption; Shrimp; Water quality. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100019 |
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| Afsar,F. Sule; Afsar,Ilhan; Diniz,Gulden; Asilsoy,Suna; Sorguc,Yelda. |
| Lupus vulgaris is the most common form of cutaneous tuberculosis which usually occurs in patients previously sensitized to Mycobacterium tuberculosis. We present a case of a 10-year-old boy who was diagnosed as lupus vulgaris clinically and histopathologically. He had well demarcated, irregularly bordered, pink, infiltrated plaques on his left cheek showing apple-jelly appearance on diascopy. The histopathological examination showed tuberculoid granulomas with Langhans type giant cells. The Mantoux reactivity was in normal limits, and no acid-fast bacilli was found in the lesion, either by direct stained smears or by culture. The lesions showed marked improvement on anti-tuberculosis treatment. We want to emphasize that histopathological examination has... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lupus vulgaris; Histopathology; Clinical appearance; Culture. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000200011 |
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| Martins,F.S.; Celestino,J.J.H.; Saraiva,M.V.A.; Chaves,R.N.; Rossetto,R.; Silva,C.M.G.; Lima-Verde,I.B.; Lopes,C.A.P.; Campello,C.C.; Figueiredo,J.R.. |
| The objective of this study was to determine the effects of GDF-9, IGF-I, and GH alone or combined on preantral follicle survival, activation and development after 1 and 7 days of in vitro culture. Either fresh (non-cultured) or cultured ovarian tissue was processed for histological and fluorescence analysis. For all media tested, the percent of normal follicles was greater when compared to minimum essential medium supplemented (MEM+) alone, except when ovarian tissue was cultured with GDF-9/IGF-I or GDF-9/GH (P < 0.05). Fluorescence analysis showed that the percent of viable follicles after 7 days of culture was similar for non-cultured tissue and for all treatments tested. The percent of primordial follicles was reduced (P < 0.05) and there was a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: GDF-9; IGF-I; GH; Culture; Follicle activation; Goat. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800006 |
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| Arana-Gabriel,Yolanda; Burrola-Aguilar,Cristina; Garibay-Orijel,Roberto; Matías-Ferrer,Noemí; Franco-Maass,Sergio; Mata,Gerardo. |
| Abstract The present study conducted a genetic characterization and determined growth rate and biomass production in solid and liquid media, using strains obtained from wild edible sporomes of Lyophyllum that grow in high mountains. Vegetative isolation was used to obtain a total of four strains, which were divided into two clades within the section Difformia: Lyophyllum sp. and Lyophyllum aff. shimeji. Growth rate and biomass production were influenced by both the culture media and the strains. In a potato dextrose agar medium, the strains presented a higher growth rate, while in a malt extract-peptone and yeast agar medium, the growth rate was lower, but with a higher biomass production that was equal to that in the malt extract-peptone and yeast liquid... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lyophyllum section Difformia; Native germplasm; Culture; Mycelium; Pellets. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300632 |
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| Carvalho,Lydston Rodrigues de; Farias,Luiz Macêdo; Nicoli,Jacques Robert; Silva,Maria Clara Fernandes; Corsino,Andrea Teresa Santos Meira; Lima,Luiz André de; Redondo,Rodrigo Aparecido Fernandes; Ferreira,Paulo César Peregrino; Pinto,Maria Elizabeth Bernardes Margutti. |
| Strict and facultative culturable anaerobic bacteria from the digestive tract of six American black vultures (Coragyps atratus Bechstein 1793) were isolated and identified. After capture, the birds received a non-contaminated diet for one week to eliminate possible allochthonous microorganisms. Then, specimens collected from tongue, stomach and intestines were weighed, submitted to decimal dilution in an anaerobic chamber, inoculated into culture media and incubated aerobically and anaerobically at 37ºC for enumeration, isolation and identification. Isolated bacteria were submitted to tests to detect possible antagonisms between them. The total bacterial population along the digestive tract ranged from 3.46 ± 0.39 log CFU/g in the stomach to 10.75 ± 0.37... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Culture; Microbiota; Digestive tract; Antagonistic substance. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300007 |
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| Silva,Francielle Gibson da; Freitas,Julio Cesar de; Anzai,Eleine Kuroki; Hashimoto,Vanessa Yumi; Giraldi,Nilson; Delbem,Ádina Cléia B.; Bracarense,Ana Paula F.R.L.; Reis,Antônio Carlos F. dos; Vasconcellos,Silvio Arruda. |
| The objective of this trial was to detect leptospires in fragments of kidney, liver and uterus of 96 cows with unknown sanitary status, randomly chosen at slaughter in Paraná, Brazil. All 96 urine samples were submitted to direct examination using dark field microscopy. Positive samples in the direct examination and all kidney, liver and uterus fragments were cultured in EMJH modified medium and Tween 80/40/LH. Sections cut of kidney, liver and uterus were stained by Hematoxylin-Eosin (HE) and indirect immunoperoxidase with hyperimmune serum against serovar Hardjo (Hardjoprajitno) and strain Londrina 14 (LO14), from serovar Canicola, which was isolated in the northern region of Paraná state, Brazil, used as primary antibodies. Direct examination detected... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospire; Indirect immunoperoxidase; Immunohistochemistry; Cows; Culture. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100008 |
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| Miraglia,Fabiana; Moreno,Andréa Mike; Gomes,Cleise Ribeiro; Paixão,Renata; Liuson,Esequiel; Morais,Zenaide Maria; Maiorka,Paulo; Seixas,Fabiana Kömmling; Dellagostin,Odir Antonio; Vasconcellos,Silvio Arruda. |
| With the aim of isolating Leptospira spp., blood serum, kidney, liver and genital tract of 137 female swine (40 sows and 97 gilts) and also urine samples from 22 sows were collected in a slaughterhouse in the State of São Paulo, from April 2003 to August 2004. Four isolates were obtained from animals that presented microagglutination test (MAT) titers > 100 for the serovar Pomona and one was obtained from an animal negative by MAT in which Leptospira was isolated from the liver and reproductive tract. The presence of leptospiral DNA was investigated by PCR, and positive results were found in kidneys of 11 females, liver of two, genital tract of two and urine of one of them. Nephrosis, interstitial multifocal nephritis, moderate to severe changing,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pomona; Swine; VNTR; Culture; PCR; Genital Tract. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300017 |
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| Başpınar,Emel Ödemiş; Dayan,Saim; Bekçibaşı,Muhammed; Tekin,Recep; Ayaz,Celal; Deveci,Özcan; Hoşoğlu,Salih. |
| Abstract Our aim in this study is to compare the standard culture method with the multiplex PCR and the Speed-Oligo® Bacterial Meningitis Test (SO-BMT) – a hybridization-based molecular test method – during the CSF examination of the patients with the pre-diagnosis of acute bacterial meningitis. For the purposes of this study, patients with acute bacterial meningitis treated at the Dicle University Medical Faculty Hospital, Infectious Diseases and Clinical Microbiology Clinic between December 2009 and April 2012 were retrospectively evaluated. The diagnosis of bacterial meningitis was made based on the clinical findings, laboratory test anomalies, CSF analysis results, and the radiological images. Growth was observed in the CSF cultures of 10 out of the 57... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial meningitis; Culture; Speed-Oligo; PCR. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200232 |
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| Marchi,A. M.; Juttel,I. D.; Kawacubo,E. M.; Dalmarco,E. M.; Blatt,S. L.; Cordova,C. M. M.. |
| Tuberculosis control is a priority for the Ministry of Health policies in Brazil. In the present work, the detection of Mycobacterium tuberculosis by the Polymerase Chain Reaction (PCR) was standardized, and the laboratory diagnosis of pulmonary tuberculosis was evaluated comparing baciloscopy, culture and PCR tests. The study was carried out with 117 sputum samples from different patients suspected of having pulmonary tuberculosis, for whom physicians had ordered a baciloscopy test. Baciloscopy was performed using the Ziehl-Neelsen method, and culture was performed by incubation of treated samples in Lowenstein-Jensen's medium at 37ºC for eight weeks. For PCR, DNA was amplified with a specific pair of primers to the M. tuberculosis complex, with a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tuberculosis; Baciloscopy; Culture; PCR. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400003 |
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| Monteiro,Paulo Henrique Tasso; Martins,Maria Conceição; Ueki,Suely Yoko Mizuka; Giampaglia,Carmen Maria Saraiva; Telles,Maria Alice da Silva. |
| The identification of Mycobacterium tuberculosis complex (MT), using non-molecular methods, is time-consuming. The objective of this study was to evaluate a screening test for the presumptive identification of MT, which could potentially decrease laboratory turn-around time for reporting preliminary results. From January 1998 to December 1999, 3056 cultures were analysed at the Mycobacterial Laboratory, Instituto Adolfo Lutz, São Paulo, Brasil. The screening test consisted of observation of colony morphology on Löwenstein Jensen medium and evaluation of cord formation on smear microscopy from those positive cultures. After the screening test, the cultures identified as non-tuberculous mycobacteria were identified to species by conventional methods (growth... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacterium tuberculosis; Identification; Cord formation; Culture. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200016 |
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| Ozbey,Gokben; Dogan,Yasar; Demiroren,Kaan; Ozercan,Ibrahim Hanifi. |
| The objectives of the present study were to determine Helicobacter pylori via culture, polymerase chain reaction and histopathological diagnosis in 101 children ranging in age from 4 to 18 years, to identify the association among restriction fragment length polymorphism types and clinical disease and to investigate the relationships among different isolates of H. pylori in different age groups. We observed a high prevalence of H. pylori infections in children between the ages of 13 and 18 (75.8%), while children aged 4 to 6 years had the lowest prevalence of infection (40%). H. pylori was detected in 30.7% (31 of 101), 66.3% (67 of 101) and 63.2% (60 of 95) of children as determined by culture methods, PCR and histological examination, respectively. H.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Children; Culture; Helicobacter pylori; PCR-RFLP. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200505 |
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| Ramis,Ivy Bastos; Moraes,Ernani Pinho de; Fernandes,Márcia Silveira; Mendoza-Sassi,Raul; Rodrigues,Obirajara; Juliano,Carlos Renan Varela; Scaini,Carlos James; Silva,Pedro Eduardo Almeida da. |
| Helicobacter pylori infects nearly 50% of the world's population. This microorganism is accepted as the most important agent of gastritis and as a risk factor for peptic ulcer disease and gastric adenocarcinoma. Currently many diagnostic methods exist for detecting H. pylori, however they all have limitations, thus it is recommend a combination of at least two methods. The aim of this study was to evaluate diagnostic methods, such as in-house urease test, culture and Polymerase Chain Reaction (PCR), for the detection of the H. pylori in gastric biopsy specimens of 144 dyspeptic patients, using as gold standard the association between histology and rapid urease test. According to the gold standard used in this study, 48 (33.3%) patients were infected with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Helicobacter pylori; Histology; Culture; Urease test; Polymerase Chain Reaction. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300008 |
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| Registros recuperados: 82 | |
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