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| Gonzales-Figueroa,H.; Gonzales-Molfino,H.M.. |
| The aim of in vitro maturation oocyte systems is to produce oocytes of comparable quality to those derived in vivo. The present study was designed to examine the surface morphological changes of the cumulus-oocyte complex (COC) and nuclear maturation in a culture system containing pyruvate. Ovaries were obtained from a slaughterhouseand transported to the laboratory within 2 h at 35-39ºC,and rinsed three times in 0.9% NaCl. The COCs were harvested from the ovaries and in vitro maturation was evaluated in San Marcos (SM) medium, a chemically defined culture system containing 22.3 mM sodium pyruvate. Oocytes were cultured in SM, SM + porcine follicular fluid (pFF) and in SM + pFF + gonadotropins (eCG and hCG) for 20-22 h and then without hormonal supplements... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Pig oocytes; In vitro maturation; Pyruvate; Cumulus cells. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600008 |
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| Picco,SJ; Seoane,A; Anchordoquy,JM; Anchordoquy,JP; Rosa,D; Fazzio,LE; Mattioli,GA; Furnus,CC. |
| Zinc is an essential trace element. It has been known that a non adequate level of zinc can alter not only gene expression but also a variety of cellular functions with severe consequences on animal health. Little is known about the role of zinc on the reproductive performance of bovines, especially in oocyte maturation. The objective of the present study was to evaluate the impact of different zinc concentrations added to culture medium, on the DNA integrity of bovine cumulus cells during in vitro maturation of oocytes. Cumulus-oocyte coplexes obtained from an abattoir were cultured in IVM mediums containing 30 (G1), 70 (G2), 110 (G3) and 150 (G4) μg/dl. At the end of IVM, cumulus cells were processed for Comet Assay. Result obtained with the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zinc; Cumulus cells; Comet assay. |
| Ano: 2008 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332008000200004 |
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