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| Boukouvala, Evridiki; Cariani, Alessia; Maes, Gregory E.; Sevilla, Rafael G.; Verrez-bagnis, Veronique; Jerome, Marc; Guarniero, Ilaria; Monios, Georgios; Tinti, Fausto; Volckaert, Filip A.m.; Bautista, José M.; Krey, Grigorios. |
| Overlapping external morphometric characters easily confound the flatfishes Solea aegyptiaca and Solea solea (Soleidae) in areas of the Mediterranean Sea where both species live in sympatry. This leads to uncertainties in the fisheries and marketing of the species, in addition to misinterpretations in biogeography and conservation studies. Here we describe a simple restriction fragment length-based diagnostic test, which differentiates S. solea from S. aegyptiaca, as well as from other species of the Soleidae family. Furthermore, we show that the two species living in sympatry in the Gulf of Kavala (North Aegean Sea, Greece) present significant qualitative differences in muscle fatty acid composition, a property which can also be used to distinguish the... |
| Tipo: Text |
Palavras-chave: Solea solea; Solea aegyptiaca; Species diagnosis; Cytochrome b gene; EcoR V restriction fragment length; White muscle fatty acid composition. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00088/19974/17641.pdf |
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| Lelievre, Stephanie; Verrez-bagnis, Veronique; Jerome, Marc; Vaz, Sandrine. |
| A better knowledge on spawning grounds of principal commercial fishes from North Sea is necessary for their monitoring. Fish egg taxonomic identification based on morphological characters cannot always be carried out effectively. This is particularly the case for a number of Gadidae fish species eggs (i.e. cod, haddock and whiting, and three rockling species) which have the same range of size and for which molecular-based techniques can help improving the accuracy of egg taxonomic identification, and hence the reliability of studies on spawning areas. In this study, formalin fixation, egg conservation and DNA extraction were optimized in order to develop a specific PCR-RFLP method. Based on the sequence of the cytochrome b gene of reference specimens, a... |
| Tipo: Text |
Palavras-chave: Fish eggs; PCR-RFLP; Cytochrome b gene; Geostatistical analyses; Interpolated map. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00005/11613/8259.pdf |
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| Tattiyapong, M.; Deemagarn, T.; Mohkeaw, K.; Ngamjiteu, S.; Jiratanh, M.. |
| Plasmodium gallinaceum and Plasmodium juxtanucleare are the most common species of Plasmodium causing avian malaria in domestic and wild chickens. In Thailand, P. gallinaceum infection in poultry has been reported since 1996, but P. juxtanucleare infection has never been reported. The objective of this study was to identify and characterize the Plasmodium species infecting Burmese red junglefowls (Gallus gallus spadiceus) in Thailand using molecular techniques. Seventeen Burmese red junglefowls from a farm in western Thailand were suspected of being infected with blood parasite. Blood samples of all seventeen Burmese red junglefowls were collected and examined. Microscopic examination revealed the presence of Plasmodium trophozoites in 5 out of 17 samples.... |
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Palavras-chave: Avian malaria; Burmese red junglefowl; Cytochrome b gene; Plasmodium juxtanucleare; Thailand. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4493 |
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