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Benabdelmouna, Abdellah; Ledu, Christophe. |
Since 2014, France’s blue mussel industry has been facing heavy mortality outbreaks (90-100%) affecting both juveniles and adults. This report presents evidence of heavy genomic abnormalities associated with mortality outbreaks in blue mussels, Mytilus edulis-galloprovincialis, from the Atlantic coast of France. In this study, ploidy characteristics of hemic cells were investigated using Flow CytoMetry (FCM), revealing an unusual, broad continuum of ploidy distribution from hypodiploidy to tetraploidy. FCM was additionally used to evaluate, at individual and populations levels, different thresholds of genomic abnormality (GA%) using the percentage of non-diploid nuclei. Individual mussels were considered to be abnormal when more than 10% of hemocytes in... |
Tipo: Text |
Palavras-chave: Mussels; Mortality; Cytogenetic quality; Hemocytes; Ploidy. |
Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00341/45171/44566.pdf |
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Benabdelmouna, Abdellah; Saunier, Alice; Ledu, Christophe; Travers, Marie-agnes; Morga, Benjamin. |
In the context of the abnormal mass mortality of mussels in France since 2014, Flow CytoMetry (FCM) was used in 2015 and 2016 to study the DNA content and cell cycle characteristics of hemic circulating cells collected from 2000 mussels. The mussels were sampled from 12 wild and cultivated blue mussels stocks distributed along the French Atlantic coast from the south Brittany to Pertuis Charentais areas. During these surveys, various genetic abnormalities were frequently detected, and ploidy characteristics revealed contrasting profiles that corresponded to respective contrasting sanitary status, i.e. healthy mussels with high cytogenetic quality (HCQ) versus diseased mussels with low cytogenetic quality (LCQ). In the present work, FCM and hemocytology... |
Tipo: Text |
Palavras-chave: Mussels; Hemocytes; Flow cytometry; Hemocytology; Cytogenetic quality; Neoplasia. |
Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00455/56623/58443.pdf |
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