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| Ramos,Daniela Fernandes; Matthiensen,Alexandre; Colvara,Wilson; Votto,Ana Paula Souza de; Trindade,Gilma Santos; Silva,Pedro Eduardo Almeida da; Yunes,João Sarkis. |
| Background The present work aimed to evaluate the antimycobacterial activity and cytotoxicity of Microcystis aeruginosa toxins, the MC-LR variant and purified extract of [D-Leu1] microcystin-LR. Methods The antimicrobial activity of M. aeruginosa extract and microcystin was evaluated by resazurin microtiter assay against Mycobacterium tuberculosis, M. terrae, M. chelonae and M. kansasii. The cytotoxicity assay was performed by trypan blue exclusion against the HTC cell line. Results Antimicrobial activity was observed in the hexanic extract of M. aeruginosa (RST 9501 strain) against M. tuberculosis, including sensitive and resistant strains with minimal inhibitory concentrations (MIC) between 1.93 μM and 0.06 μM. The high activity of M. aeruginosa hexanic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacteria; Antimycobacterial agents; Cytotoxic activity; Microcystins. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100315 |
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| MIYAHARA, Kazuro; TOSE, Shinichi; SAKURAI, Haruhisa; IGARASHI, Ikuo; SAITO, Atsushi; HIROSE, Tsuneo; SUZUKI, Naoyoshi; 宮原, 和郎; 五十嵐, 郁男. |
| In order to identify mechanisms responsible for the anti-tumor effects of Toxoplasma lysate antigen (TLA), we used an in vitro 51Cr release assay to study the functional properties of plastic-adherent cells during induction of splenic cytotoxic activity by TLA. Cytotoxic activity of non-adherent cells was measured in all experiments after a 6 days incubation. Induction of cytotoxic non-adherent cells by TLA required the presence of plastic-adherent spleen cells. In contrast, rhIL-2 alone was able to induce transformation of cytotoxic non-adherent cells from non-adherent spleen cells. Contact between adherent and non-adherent spleen cells was necessary for successful induction of cytotoxic non-adherent cells by TLA. Treatment of spleen cells with... |
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Palavras-chave: Adherent cell; Cytotoxic activity; Macrophage; Spleen cell; Toxoplasma lysate antigen. |
| Ano: 1992 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4065 |
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| MIYAHARA, Kazuro; MATSUMOTO, Toru; TOSE, Shinichi; SAKURAI, Haruhisa; IGARASHI, Ikuo; SAITO, Atsushi; HIROSE, Tsuneo; SUZUKI, Naoyoshi; 宮原, 和郎; 五十嵐, 郁男. |
| Spleen cells from Toxoplasma lysate antigen (TLA)-sensitized BALB/c mice showed the strong cytotoxic activity against both natural killer (NK)-sensitive cells (YAC-1 and RL♂-1) and NK-insensitive cells (P-815), when incubated with TLA or recombinant human IL-2 (rhIL-2). The increment of TLA concentration in culture medium increased the cytotoxic activity. Treatment of effector cells; spleen cells from TLA-sensitized mice incubated with TLA, with anti-asialo GM1 or anti-Thy-1 plus complement inhibited the cytotoxic activity of effector cells, whereas treatment with anti-mouse Lyt-2.2 serum plus complement had no effect on the cytotoxic activity. Treatment of spleen cells from TLA-sensitized mice with anti-asialo GM1 and/or anti-Thy-1 plus complement... |
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Palavras-chave: Cytotoxic activity; Mouse; Phenotype; Spleen cell; Toxoplasma lysate antigen. |
| Ano: 1992 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4063 |
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| RICARTE,LARA P.; BEZERRA,GABRIELI P.; ROMERO,NIRLA R.; SILVA,HORLANDO C. DA; LEMOS,TELMA L.G.; ARRIAGA,ANGELA M. C.; ALVES,PÉRICLES B.; SANTOS,MARCELO B. DOS; MILITÃO,GARDENIA C.G.; SILVA,THIAGO D.S.; BRAZ-FILHO,RAIMUNDO; SANTIAGO,GILVANDETE M.P.. |
| Abstract The essential oils obtained by hydrodistillation from fresh leaves of Vitex agnus-castus and Ocimum campechianum, and from fresh inflorescences of Ocimum carnosum were analysed by GC-FID and GC-MS. The major components of V. agnus-castus essential oil were identified as 1,8-cineole (47.9%), terpinyl α-acetate (11.6%), sabinene (11.2%) and caryophyllene oxide (9.7%), while in the O. campechianum essential oil were eugenol (72.1%), β-elemene (6.8%), (E)-caryophyllene (6.4%) and bicyclogermacrene (5.2%). Linalool (79.0%), α-epi-cadinol (5.4%), terpinen-4-ol (3.2%) and 1,8-cineole (2.8%) were the major constituents in the O. carnosum essential oil. The essential oils were subsequently evaluated for their larvicidal and cytotoxic activities. Larval... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aedes aegypti; Cytotoxic activity; Lamiaceae; Larvicidal activity. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000100718 |
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| ANDRADE,PRISCILA M. DE; MELO,DAIANA C. DE; ALCOBA,ANA ELISA T.; FERREIRA JÚNIOR,WALNIR G.; PAGOTTI,MARIANA C.; MAGALHÃES,LIZANDRA G.; SANTOS,TAINÁ C.L. DOS; CROTTI,ANTÔNIO E.M.; ALVES,CASSIA C.F.; MIRANDA,MAYKER L.D.. |
| Abstract Leishmaniasis is an endemic disease caused by protozoa of the genus Leishmania, which affects around two million people worldwide. One major drawback in the treatment of leishmaniasis is the emergence of resistance to current chemotherapeutics. Medicinal and aromatic plants constitute a major source of natural organic compounds. In this study, the leaf essential oil of Cryptocarya aschersoniana was obtained by hydrodistillation in a Clevenger-type apparatus, and the chemical composition was analyzed by GC-MS and GC-FID. The essential oil of these species was predominantly constituted by monoterpene hydrocarbons (48.8%). Limonene (42.3%), linalool (9.7%) and nerolidol (8.6%) were the main constituents in the oil of C. aschersoniana. The in vitro... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cryptocarya aschersoniana; Lauraceae; Essential oil; Leishmania amazonensis; Cytotoxic activity. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000602671 |
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| KHANAVI,MAHNAZ; NABAVI,MARYAM; SADATI,NARGESS; SHAMS ARDEKANI,MOHAMMADREZA; SOHRABIPOUR,JELVE; NABAVI,SEYED MOHAMMAD B; GHAELI,PADIDEH; OSTAD,SEYED NASSER. |
| The aim of this study was to investigate the in vitro cytotoxic activity of total extract of MeOH (70%) and partition fractions of hexan, chloroform (CHCL3), ethylacetate (EtOAc) and MeOH-H2O of brown algae species (Sargassum swartzii, Cystoseira myrica, Colpomenia sinuosa) found in the Persian Gulf against in different cell lines including HT-29, Caco-2, T47D, MDA-MB468 and NIH 3T3 cell lines by MTT and AnnexinV-PI assay. The hexan fraction of S. swartzii and C. myrica showed selective cytotoxicity against proliferation of Caco-2 cells (IC50<100 μg/ml) T47D cell line (IC50<100 μg/ml), respectively. S. swartzii and C. myrica were also observed for increasing apoptosis in Caco-2 and T47D cells. Total extract and fractions of C.... |
| Tipo: Journal article |
Palavras-chave: Colpomenia sinuosa; Cystoseira myrica; Cytotoxic activity; MTT assay; Persian Gulf; Sargassum swartzii. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100005 |
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| Rosas,L.V.; Cordeiro,M.S.C.; Campos,F.R.; Nascimento,S.K.R.; Januário,A.H.; França,S.C.; Nomizo,A.; Toldo,M.P.A.; Albuquerque,S.; Pereira,P.S.. |
| Ampelozizyphus amazonicus Ducke is a tree commonly found in the Amazon region and an extract of its stem bark is popularly used as an antimalarial and anti-inflammatory agent and as an antidote to snake venom. Ursolic acid; five lupane type triterpenes: betulin, betulinic acid, lupenone, 3ß-hydroxylup-20(29)-ene-27,28-dioic acid, and 2a,3ß-dihydroxylup-20(29)-ene-27,28-dioic acid, and three phytosteroids: stigmasterol, sitosterol and campesterol, have been isolated from stem extracts of A. amazonicus Ducke. Their structures were characterized by spectral data including COSY and HMQC. In an in vitro biological screening of the isolated compounds, 3ß-hydroxylup-20(29)-ene-27,28-dioic acid was cytotoxic against the SKBR-3 human adenocarcinoma cell line (1 to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ampelozizyphus amazonicus; Rhamnaceae; Pentacyclic triterpenes; Cytotoxic activity; Trypanocidal activity. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500009 |
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| Almeida,Tamires Cunha; Seibert,Janaina Brandão; Almeida,Sávio Henrique de Souza; Amparo,Tatiane Roquete; Teixeira,Luiz Fernando de Medeiros; Barichello,Jose Mario; Postacchini,Bruna Bueno; Santos,Orlando David Henrique dos; Silva,Glenda Nicioli da. |
| Antimicrobial and antitumor activities of resveratrol, a compound found mainly in grapes, have already been demonstrated. However, its low bioavailability is a limiting factor for therapeutic application. Polymeric micelles can be an approach to solve this problem since they can encapsulate hydrophobic substances. We developed and characterized micellar formulations containing resveratrol and evaluated their cytotoxic and antimicrobial effects. The formulations were prepared by the cold dispersion method with different concentrations of F127 (5 or 10% w/w) and resveratrol (500 or 5000 µM). The formulations were characterized according to size, polydispersity index, pH, encapsulation rate and in vitro release. Cytotoxic effect was evaluated on a bladder... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antimicrobial activity; Cytotoxic activity; F127®; Polymeric micelles; Resveratrol. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100567 |
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