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	Provedor de dados ArchiMer (2) Biol. Res. (1) Ciência e Agrotecnologia (1) Genet. Mol. Biol. (1) Rev. Bras. Frutic. (1) Autor Leroy, Celine (2) Combes, D (1) Compere, Chantal (1) DEL RÍO-PORTILLA,MIGUEL ÁNGEL (1) DUPRÉ,ENRIQUE (1) Dalkiliç,Gonca Günver (1) Dalkiliç,Zeynel (1) Davide,Lisete Chamma (1) Delbarre Ladrat, Christine (1) Dias,Eustáquio Souza (1) Faria,Karina de Cassia (1) GALLARDO-ESCÁRATE,CRISTIAN (1) Ghillebaert, F (1) Labory,Cláudia Regina Gontijo (1) Morielle-Versute,Eliana (1) Rochet, Marie-joelle (1) Schawan,Rosane Freitas (1) VON BRAND,ELISABETH (1) Wenzel,Inajá Marchizeli (1) ÁLVAREZ-BORREGO,JOSUÉ (1) Mais... Palavra-chave DAPI (6) Adhesion (2) Antifouling (2) Marine biofilm (2) Microtiter plate (2) Screening (2) Adhésion (1) Agaricus blazei (1) AluI (1) Antisalissure (1) Biofilm marin (1) Calcofluor (1) Chiroptera (1) Citogenética (1) Criblage (1) DNA content (1) EPS (1) FISH (1) Ficus carica (1) Flow cytometry (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Text (2) Journal article (1) Ano 2018 (1) 2007 (2) 2006 (1) 2003 (1) 2002 (1) País Brazil (3) France (2) Chile (1) Idioma Inglês (4) Francês (1) Português (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 6 Primeira ... 1 ... Última In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu I Genet. Mol. Biol. Faria,Karina de Cassia; Morielle-Versute,Eliana. The purpose of this work was to verify the ability of the enzyme Alu I to cleave and/or remove satellite DNA sequences from heterochromatic regions in chromosomes of bats, by identifying the occurrence of modifications in the pattern of fluorescence in situ hybridization with telomeric DNA. The localization and fluorescence intensity of the telomeric DNA sites of the Alu-digested and undigested chromosomes of species Eumops glaucinus, Carollia perspicillata, and Platyrrhinus lineatus were analyzed. Telomeric sequences were detected at the termini of chromosomes of all three species, although, in C. perspicillata, the signals were very faint or absent in most chromosomes. This finding was interpreted as being due to a reduced number of copies of the... Tipo: Info:eu-repo/semantics/article Palavras-chave: AluI; FISH; Chiroptera; Telomeric sites; DAPI. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400003 Lutte contre les salissures marines : approche par procédés enzymatiques. ArchiMer Leroy, Celine. Fouling on marine underwater surfaces causes critical and economic problems such as important material biodamages and industrial performances reduction. We chose to test antifouling potential of enzymatic commercial preparations like hydrolases (proteases, glycosidases and lipases) in order to inhibit the first fouling adhesion step: bacterial biofilm formation. An evaluation test of antifouling properties onto marine bacterial adhesion was designed using a mono-incubation of Pseudoalteromonas sp. D41 in microtiter plate and in sterile natural sea water. This test was adapted to screen agents for bacterial adhesion removal or inhibition activities and allowed to test enzymatic preparations toxicity on non adhered bacteria. Inhibition rates according to... Tipo: Text Palavras-chave: Fouling; Adhesion; Antifouling; Hydrolases; EPS; DAPI; Microtiter plate; Screening; Pseudoalteromonas sp.; Marine biofilm; Salissure marine; Adhésion; Antisalissure; Hydrolases; EPS; DAPI; Microplaque; Criblage; Pseudoalteromonas sp.; Biofilm marin. Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/these-1099.pdf A marine bacterial adhesion microplate test using the DAPI fluorescent dye: a new method to screen antifouling agents ArchiMer Leroy, Celine; Delbarre Ladrat, Christine; Ghillebaert, F; Rochet, Marie-joelle; Compere, Chantal; Combes, D. Aims: To develop a method to screen antifouling agents against marine bacterial adhesion as a sensitive, rapid, quantitative microplate fluorescent test. Methods and Results: Our experimental method is based on a natural biofilm formed by mono-incubation of the marine bacterium Pseudoalteromonas sp. D41 in sterile natural sea water in a 96 wells polystyrene microplate. The 4'6-diamidino-2-phenylindole (DAPI) dye was used to quantify adhered bacteria in each well. Total measured fluorescence in wells was correlated with the amount of bacteria showing a detection limit of one bacterium per 5 µm2 and quantifying 2 x 107 to 2 x 108 bacteria adhered per cm2. The antifouling properties of three commercial surface active agents and chlorine were tested by this... Tipo: Text Palavras-chave: Antifouling; Pseudoalteromonas; Screening; Microtiter plate; Adhesion; DAPI; Marine biofilm. Ano: 2007 URL: http://archimer.ifremer.fr/doc/2006/publication-2017.pdf DNA content estimation of Fig and Black Mulberry using flow cytometry Rev. Bras. Frutic. Dalkiliç,Zeynel; Dalkiliç,Gonca Günver. Abstract In this study, fig and black mulberry DNA contents were estimated using DAPI fluorescence stain in flow cytometry. The 2C DNA contents of the fig and black mulberry were found as 0.82 pg and 8.34 pg, respectively. The calculated 1C value of genome size of fig is 401.8 Mbp and that of black mulberry is 4086.6 Mbp. The ratio of 2C DNA content and 1C genome of the black mulberry was 10.17 times that of the fig although fig is diploid and black mulberry is decosaploid. Tipo: Info:eu-repo/semantics/article Palavras-chave: Ficus carica; Morus nigra; DAPI; DNA content; Flow cytometry; Genome size. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000200801 Relationship between DAPI-fluorescence fading and nuclear DNA content: An alternative method to DNA quantification? Biol. Res. GALLARDO-ESCÁRATE,CRISTIAN; ÁLVAREZ-BORREGO,JOSUÉ; VON BRAND,ELISABETH; DUPRÉ,ENRIQUE; DEL RÍO-PORTILLA,MIGUEL ÁNGEL. In observations by confocal or conventional fluorescence microscopy, important factors should be considered in order to obtain accurate images. One of them, such as the fluorescence bleaching from highest intensity to lowest signal of fluorescence is a common problem with several DNA fluorochromes and especially for DAPI stain. The fluorescence of DAPI fades rapidly when it is exposed to UV light, under optimal conditions of observation. Although the fading process can be retarded using a mounting medium with antifading reagents, the photochemical process underlying the fluorescence decay has not yet been fully explained. In addition, no relationship between fluorescence fading and nuclear DNA content has been tested. In order to test this relationship, we... Tipo: Journal article Palavras-chave: Nuclear DNA content; Fluorescence fading; Image analysis; DAPI. Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100004 Coloração de núcleos de esporos e hifas do cogumelo Agaricus blazei Ciência e Agrotecnologia Labory,Cláudia Regina Gontijo; Dias,Eustáquio Souza; Davide,Lisete Chamma; Schawan,Rosane Freitas; Wenzel,Inajá Marchizeli. Com o objetivo de se estudar o comportamento nuclear durante o ciclo sexual do cogumelo Agaricus blazei , técnicas de coloração e a utilização de fluorocromos foram testadas, visando a uma padronização metodológica para estudos citogenéticos futuros. Das técnicas avaliadas neste trabalho (método de Feulgen, coloração com Giemsa e fluorescência-DAPI), a coloração com Giemsa e o fluorocromo DAPI apresentaram os melhores resultados para evidenciar núcleos. O corante Giemsa permitiu a visualização de compartimentos multinucleados. Com o DAPI, não foi possível a visualização dos septos, sendo necessária a utilização adicional de calcofluor, que tem a propriedade de corar a quitina que delimita os compartimentos das hifas. Tipo: Info:eu-repo/semantics/article Palavras-chave: Agaricus blazei; Giemsa; DAPI; Calcofluor; Citogenética. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542003000200030 Registros recuperados: 6 Primeira ... 1 ... Última

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