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	Provedor de dados Infoteca-e (54) Open Agri (18) Genet. Mol. Biol. (12) ArchiMer (11) Arq. Bras. Med. Vet. Zootec. (9) Naturalis (6) BJMBR (6) OceanDocs (5) Colegio de Postgraduados (5) BABT (5) Ciência Rural (5) R. Bras. Zootec. (5) Anais da ABC (AABC) (4) Rev. Bras. Frutic. (4) Trop. Plant Pathol. (3) Thai Agricultural (3) BJM (3) Ciência e Agrotecnologia (3) Electron. J. Biotechnol. (3) Planta Daninha (3) Mais... Autor CORDEIRO, M. C. R. (4) Compere, Chantal (4) Oliveira,D.A.A. (4) Alves,C. (3) Cardoso,L.R. (3) Coelho,E.G.A. (3) FALEIRO, F. G. (3) Fonseca,A.S. (3) KARIA, C. T. (3) Martins,D. (3) Mori,E.S. (3) Paoli,F. (3) Renault, Tristan (3) Rodrigues,S.G. (3) Teixeira,C.S. (3) ANDRADE, R. V. de (2) Ali,Hanaa Mahmoud (2) Antoine, Elisabeth (2) BALDANI, J. I. (2) BONATO, A. L. V. (2) Mais... Palavra-chave DNA (189) PCR (17) Genomes (12) Biotecnologia (10) Genes (10) Genetic structures (9) Marcador Molecular (9) Polymorphism (9) Bovino (8) Extração (8) RAPD (8) Biologia Molecular (7) Chickpeas (7) Genetic markers (7) Biotechnology (6) Genotypes (6) Hybrids (6) Marcador Genético (6) RNA (6) Cloning (5) Mais... Tipo do documento Info:eu-repo/semantics/article (71) Folhetos (30) Text (11) Article / Letter to the editor (6) Gravações de som (6) Artigo na mídia (5) Folders (5) Journal article (5) Journal Contribution (4) Info:eu-repo/semantics/other (3) Info:eu-repo/semantics/report (3) Parte de livro (3) Livros (2) Theses and Dissertations (2) Collection (1) Fascículos de periódicos (1) Libro (1) Material não-convencional (1) Peer-Reviewed Reports (1) PhysicalObject (1) Mais... Ano 2022 (5) 2021 (1) 2020 (3) 2019 (10) 2018 (5) 2017 (4) 2016 (5) 2015 (6) 2014 (4) 2013 (11) 2012 (7) 2011 (2) 2010 (11) 2009 (3) 2008 (12) 2007 (17) 2006 (11) 2005 (17) 2004 (6) 2003 (12) Mais... País Brazil (129) India (18) France (11) Belgium (6) Netherlands (6) Chile (5) Mexico (5) Thailand (3) Colombia (2) Canada (1) Japan (1) Ukraine (1) United States (1) Mais... Idioma Português (79) Inglês (71) Espanhol (9) Thailandês (3) Francês (2) Russo (1) Mais...		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 189 Primeira ... 1 2 3 4 5 6 7 8 9 ... Última GENETIC VARIABILITY AND REPRODUCTIVE CHARACTERISTICS OF ZEBRAFISH (Cyprinidae Danio rerio) STOCKS Acta biol.Colomb. LEWANDOWSKI,Vanessa; SARY,Cesar; ETTA,Jaisa CAS; SOUZA,Felipe Pinheiro de; CASTRO,Pedro Luiz de; GOES,Elenice Souza dos Reis; OLIVEIRA,Carlos Antonio Lopes de; RIBEIRO,Ricardo Pereira; VARGAS,Lauro. ABSTRACT Specimens of cultured zebrafish acquired from different fish farms in Brazil may show genetic variability and alteration in allele frequency due to genetic drift and selective pressure in a captive environment, resulting in the differentiation of productive and reproductive characteristics. The aim of this study was to evaluate the genetic variability and reproductive characteristics of 180 zebrafish specimens from six Brazilian fish farms. A deviation from the Hardy-Weinberg equilibrium was observed in all evaluated stocks. Differentiation among stocks was observed in the amount of genetic variability with respect to observed heterozygosity and the inbreeding coefficient (FIS). Genetic distance between stocks was determined through the Fst index,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Animal model; Consanguinity; DNA; Female; Fish larvae. Ano: 2022 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2022000200223 LA DUALIDAD ANÁLOGO DIGITAL DE LA INFORMACIÓN SE EJEMPLIFICA EN EL ESTUDIO DE LAS MÓLECULAS DE RNA Acta biol.Colomb. ANDRADE,EUGENIO. Las investigaciones sobre las moléculas de RNA, han contribuido a precisar y formalizar la noción física de información considerada tanto en su aspecto analógico como digital. El dogma central de la biología molecular, restringe la noción de información al aspecto digital de la misma, y por tanto al copiado fiel de una cadena de símbolos en los procesos de replicación (DNA-DNA) y transcripción (DNA-RNA), e igualmente en la traducción a proteínas mediante el código genético. Esta visión genera la paradoja sobre qué surgió primero si DNA o proteínas, la cual se resuelve en parte recurriendo a la hipótesis según la cual existió un mundo prebiótico RNA, anterior a las proteínas codificadas genéticamente y al DNA. Esta hipótesis tiene soporte empírico sólido... Tipo: Info:eu-repo/semantics/article Palavras-chave: RNA; DNA; Proteínas; Información digital; Información analógica; Dogma central; Mundo RNA. Ano: 2011 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2011000300002 Comparação de protocolos para extração do DNA de Lernaea sp. (Copepoda: Cyclopoida) - DOI: 10.4025/actascianimsci.v25i2.1973 Animal Sciences Valentim, Marivone; UFP; Vargas, Lauro; UEM; Moreira, Héden Marques; UEM; Ribeiro, Ricardo Pereira; UEM. Neste estudo, 30 amostras de fêmeas adultas de Lernaea sp. foram obtidas de peixes naturalmente infestados, em 2 pisciculturas na região norte do estado do Paraná, Brasil. Testaram-se 2 protocolos de extração do DNA para os tecidos do parasito. Os resultados obtidos revelaram o protocolo, utilizando o “Kit Puregene DNA Isolation” (GENTRA) para tecidos sólidos, como adequado para extração do DNA genômico de Lernaea. O método, utilizando protocolo CTAB, descrito por Black IV et al. (1996), modificado para a extração do DNA de Lernaea sp., foi tido como não-adequado, pois não houve extração do DNA. Palavras-chave: 5.04.00.00-2 Zootecnia Crustacea; Lernaea; DNA; Protocolos de extração 5.04.00.00-2 Zootecnia. Ano: 2003 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/1973 Engineering biodiversity as a model for the species conservation Anais da ABC (AABC) RECH,ELIBIO L.. Abstract: Early humans have domesticated plant and animal species based on ancient empirical concepts (Darwin 1868, 1876). In 1886, Mendel established a new paradigm of hereditary laws (Mendel 1866, 1870, 1950) based on genotypic and phenotypic traits of cross-compatible species, establishing a complex breeding technology that is currently utilized for the development of most food and livestock-derived products. Recently, studies on deciphering the double-helical structure (Watson and Crick 1953a, b) and how to restrict DNA (Arber 2012) have established the foundation of recombinant DNA technology. A new era is paving the way for genetic manipulation of important traits among all the kingdom’s organisms, allowing for the development of innovative and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodiversity; Species conservation; DNA; Genetic engineering. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000600617 Campomanesia adamantium (Cambess.) O. Berg seed desiccation: influence on vigor and nucleic acids Anais da ABC (AABC) DRESCH,DAIANE M.; MASETTO,TATHIANA E.; SCALON,SILVANA P.Q.. The aim of this study was to evaluate the sensitivity of Campomanesia adamantium seeds to desiccation by drying in activated silica gel (fast) and under laboratory conditions (slow). To assess the sensitivity of the seeds to desiccation, we used drying with silica gel and drying under laboratory conditions (25 °C), in order to obtain seeds with moisture content of 45, 35, 30, 25, 20, 15, 10 and 5%. The physiological potential of the seeds after desiccation was evaluated by measuring primary root protrusion, percentage of normal seedlings, germination seed index, seedling length, total seedling dry mass, electrical conductivity and DNA and RNA integrities. The C. adamantium seeds were sensitive to desiccation and to a reduction in moisture content to 21.1%... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cerrado; DNA; Drying; RNA; Viability. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000502217 Molecular research and the control of Chagas disease vectors Anais da ABC (AABC) Abad-Franch,Fernando; Monteiro,Fernando A.. Chagas disease control initiatives are yielding promising results. Molecular research has helped successful programs by identifying and characterizing introduced vector populations and by defining intervention targets accurately. However, researchers and health officials are facing new challenges throughout Latin America. Native vectors persistently reinfest insecticide-treated households, and sylvatic triatomines maintain disease transmission in humid forest regions (including Amazonia) without colonizing human dwellings. In these scenarios, fine-scale vector studies are essential to define epidemiological risk patterns and clarify the involvement of little-known triatomine taxa in disease transmission. These eco-epidemiological investigations, as well as... Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular systematics; DNA; Allozymes; Triatominae; Chagas disease. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652005000300007 Molecular survey of Anaplasma platys and Ehrlichia canis in dogs from Campo Grande, Mato Grosso do Sul, Brazil Anais da ABC (AABC) SOARES,RODRIGO; RAMOS,CARLOS ALBERTO; PEDROSO,THATIANNA; BABO-TERRA,VERÔNICA; CLEVELAND,HERBERT; ARAÚJO,FLÁBIO DE. ABSTRACT This study investigated the frequency of infection by Anaplasma platys and Ehrlichia canis in dogs submitted to animal health centers in Campo Grande, state of Mato Grosso do Sul, Brazil. E. canis and A. platys showed infection frequencies of 55.75% and 16.96%, respectively. The identity of the two species was confirmed by DNA sequencing. Tipo: Info:eu-repo/semantics/article Palavras-chave: Epidemiology; NPCR; DNA; Midwestern Brazil.. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000100301 Randon amplified polymorphic DNA in the analysis of isolates of Haemonchus (Nematoda-Trichostrongylidae) originated from bovines from different regions Arq. Bras. Med. Vet. Zootec. Guimarães,M.P.; Gomes,M.A.; Caldeira,M.C.. Utilizou-se a técnica de DNA polimórfico amplificado ao acaso (RAPD) para a análise de 14 amostras de Haemonchus, um tricostrongilídeo parasito do abomaso de bovinos, originárias de diferentes regiões do estado de Minas Gerais. Os perfis foram obtidos utilizando-se cinco iniciadores diferentes. Não houve correlação entre polimorfismo observado com a origem do animal ou com a espécie de Haemonchus estudada. Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattle; RAPD; Haemonchus; DNA. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000100019 Detection of mutations within exons 4 to 8 of the p53 tumor suppressor gene in canine mammary glands Arq. Bras. Med. Vet. Zootec. Souza,D.M.B.; Barros,M.G.O.; Silva,J.S.C.; Silva,M.B.; Coleto,Z.F.; Jimenez,G.C.; Adrião,M.; Wischral,A.. Fifteen female canines with mammary tumors and 6 normal females were used to study mutations in exons 4 to 8 of the p53 gene. DNA samples from the tumors, respective adjacent normal mammary tissue and mammary glands from healthy animals were sequenced and analyzed for the presence of mutations. Mutations were found in 71.8% of the samples and the most frequent were missense mutations. The most attacked exons in the mammary tumor were 5, 7 and 8, with 23.4, 31.6 and 23.4% mutations, respectively. Canine mammary tumors are related to mutations in gene p53 and mutations mostly occur in the region of the protein that is linked to the DNA in the cell nucleus, which can change the functionality of the cell and propitiate tumor growth. Despite being... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bitch; DNA; Mamma; Molecular biology; Neoplasm; PCR. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000200013 Teste de DNA para verificação de parentesco em cães: avaliação do método não automatizado com o auxílio do primer CMR S Arq. Bras. Med. Vet. Zootec. Oliveira,P.F.; Oliveira,D.A.A.; Teixeira,C.S.; Velloso,A.P.S.; Coelho,E.G.A.; Rodrigues,S.G.; Alves,C.. To evaluate the precision of the DNA tests using the non-automatized technique for individual identification and parentage tests, 105 Rottweiler dogs were studied using the primer CMR S. The sample was composed of 39 animals belonging to 11 complete families and their progenies, and 66 non related individuals until the second generation, derived from kennels located in the states of Minas Gerais and São Paulo. The CMR S primer was used for the Polimerase Chain Reaction (PCR). The results showed the inefficiency of the technique, even when analyzed through the automated gel analysis system. Also showed the impossibility of its commercial use due to the fact of does not permit the storage of data for subsequent use. Tipo: Info:eu-repo/semantics/other Palavras-chave: Cão; DNA; Teste de verificação de parentesco. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000500018 Padronização de uma PCR para a autenticação do Salmo salar em pratos da culinária japonesa Arq. Bras. Med. Vet. Zootec. Gonçalves,E.P.M.; Barros,M.C.S.; Pessôa,M.C.; Cardilli,D.J.; Roos,T.B.; Moraes,C.M.. RESUMO O objetivo deste trabalho foi padronizar uma PCR para a detecção do Salmo salar, a qual possa ser usada na autenticação do salmão utilizado em pratos da culinária japonesa e do pescado comercializado in natura. Para isso, dois lotes de sushi foram produzidos experimentalmente. Além disso, foram visitados 38 estabelecimentos que comercializam comida japonesa e 10 peixarias na região metropolitana de Belém, visando à coleta do sushi, do temaki e do pescado pertencente à espécie Salmo salar. Os dados demonstraram que a técnica foi eficiente para a autenticação de Salmo salar, visto que a espécie foi detectada tanto nas amostras de sushis preparados experimentalmente quanto nas alíquotas de pescados isolados, utilizados para a preparação do sushi. Em... Tipo: Info:eu-repo/semantics/article Palavras-chave: Sushi; Pescado; Temaki; Identificação; DNA. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200640 Sexagem em tamanduá-bandeira (Myrmecophaga tridactyla) por meio do teste da reação em cadeia da polimerase Arq. Bras. Med. Vet. Zootec. Bento,H.J.; Rosa,J.M.A.; Morgado,T.O.; Granjeiro,M.D.B.; Bianchini,M.A.; Iglesias,G.A.; Dutra,V.; Nakazato,L.; Paz,R.C.R.. RESUMO Em tamanduá-bandeira (Myrmecophaga tridactyla) não há dimorfismo sexual, tornando-se necessária a diferenciação entre machos e fêmeas, em especial naqueles indivíduos com finalidade reprodutiva. Entre as diversas técnicas empregadas para a caracterização sexual, a reação em cadeia da polimerase (PCR) é utilizada em mamíferos para identificar uma sequência genética especifica do cromossomo Y (SRY), sendo considerado um meio moderno e eficaz de determinação sexual. O objetivo deste trabalho é padronizar um protocolo para determinação sexual de tamanduá-bandeira por meio da técnica de PCR, utilizando material genético extraído do bulbo capilar desses animais. Mediante esse protocolo, foi possível determinar o sexo de sete animais testados, sendo... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pilosa; Reprodução; Conservação; Determinação sexual; DNA. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200538 Microssatélites BM2113, ILSTS005, ILSTS008, ETH131 e RM88 em testes de verificação de parentesco para bovinos da raça Gir Arq. Bras. Med. Vet. Zootec. Rodrigues,S.G.; Oliveira,D.A.A.; Teixeira,C.S.; Oliveira,P.F.; Coelho,E.G.A.; Alves,C.; Velloso,A.P.S.; Pereira,J.C.C.. Foram utilizados 46 animais da raça Gir, registrados na Associação Brasileira de Criadores de Zebu, provenientes de cinco fazendas situadas no Estado de Minas Gerais, com o objetivo de avaliar a eficiência dos microssatélites BM2113, ILSTS005, ILSTS008, ETH131 e RM88 em testes de verificação de parentesco. Os locos BM2113, ILSTS005, ETH131 e RM88 mostraram-se eficientes, apresentando valores de PE2 (probabilidade de exclusão quando os dois progenitores são genotipados) entre 0,62 e 0,69 e PIC2 (conteúdo de informação polimórfica quando os dois progenitores são genotipados) entre 0,78 e 0,83. O mesmo não ocorreu para o loco ILSTS008, o qual apresentou baixos valores de PE2 (0,24) e PIC2 (0,41). Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovino; Microssatélite; DNA. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000300015 Comparação entre métodos de estocagem de DNA extraído de amostras de sangue, sêmen e pêlos e entre técnicas de extração Arq. Bras. Med. Vet. Zootec. Coelho,E.G.A.; Oliveira,D.A.A.; Teixeira,C.S; Sampaio,I.B.M.; Rodrigues,S.G.; Alves,C.. DNA samples of six bovines obtained from three tissues (blood, semen and hair) were extracted using two different techniques. After the extraction procedures the samples were divided in six fractions. Three were stored at -20° C and three at 4° C. Every three months one sample of each tissue/extraction procedure was analyzed in spectrophotometer, to determine the quantity of the DNA and the extract was amplified using the primer RM 29. No differences in the DNA quantity or in the level of protein contamination among the three periods of analyses were observed. All the DNA extracted by quick extraction technique showed good amplification patterns during the nine months, meaning that this technique can be used in laboratory routine instead of the permanent... Tipo: Info:eu-repo/semantics/other Palavras-chave: DNA; Métodos de extração; Amplificação. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000100017 Bovine alphaherpesvirus 1 and 5 in semen from bulls presenting genital lesions under field conditions in Brazil Arq. Bras. Med. Vet. Zootec. Henzel,A.; Salla,P.F.; Mascitti,A.K.; Demoliner,M.; Solyman,M.C.; Lunge,V.R.; Spilki,F.R.. ABSTRACT Bovine alphaherpesviruses 1 and 5 (BoHV-1/5) are main pathogens of respiratory, reproductive and neurological diseases in cattle. The aim of this study was to investigate the frequency of neutralizing antibodies against BoHV-1/5 in serum samples and to detect viral DNA in semen of bulls from beef cattle farms located in RS. A total of 372 serum and semen sample from bulls were collected in eighteen farms. Serum samples were submitted to virus neutralization (VN) assay, while semen samples were used to detect BoHV-1 and BoHV-5 DNA by PCR. VN results showed that BoHV-1/5 antibodies were detected in bulls of 66.7% (12/18) of the farms, 295 (79.5%) BoHV positive bulls, 287 for BoHV-1 and 234 for BoHV-; at 43 vaccinated bulls 72.1% (31/43) showing... Tipo: Info:eu-repo/semantics/article Palavras-chave: BoHV-1 and 5; Co-infection; DNA; Semen. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100197 Freqüência do gene Miostatina (GDF-8) em rebanhos brasileiros da raça Marchigiana Arq. Bras. Med. Vet. Zootec. Teixeira,C.S.; Oliveira,D.A.A.. Identificou-se e determinou-se a freqüência do gene miostatina (GDF-8) normal e mutante em rebanhos Marchigiana, em 377 bovinos da raça Marchigiana, criados nos estados de São Paulo e Paraná. Identificaram-se 37,9% de animais normais, 55,2% de portadores e 6,9% homozigotos afetados para musculatura dupla. Estes resultados indicam que os criadores têm interesse na característica musculatura dupla, promovendo, ainda que aleatoriamente, selecão a favor da mutação. Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovino; Marchigiana; DNA; Miostatina; Musculatura dupla; PCR. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000300036 Alterations in Biomarkers Associated with Sterility in Pectinophora gossypiella (Saunders) Induced by Gamma Irradiation BABT Ali,Hanaa Mahmoud; Moustafa,Hemat Zakaria; Sayed,Rehab Mahmoud. ABSTRACT Nowadays, radiation technology is widely used to produce changes in Biosystems. The goal of this work is to determine the variation induced male Pectinophora gossypiella in gamma-irradiated as pupae using 50Gy and 150Gy. Comparing elements composition and DNA (using RAPD-PCR) between substerile 50Gy and the sterile dose 150Gy in P. gossypiella showed variation between them. Potassium (K) was the most abundant elements in unirradiated and irradiated males followed by magnesium (Mg). The percentage of heavy metals as copper, zinc, and cadmium concurrent with K was directly proportional to the radiation dose. While the percentage of Mg, Phosphorous and calcium decreased as the radiation dose increased. The results also revealed that some extra bands... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pectinophora gossypiella; Gamma rays; RAPD-PCR; Elements; DNA. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100436 A simplified DNA extraction method for PCR analysis of Camarotella spp. BABT Vitória,Nadja Santos; Bezerra,José Luiz; Gramacho,Karina Peres. This work aimed to optimize an efficient and simple protocol for DNA extraction of Camarotella species, an obligate plant pathogen that cause verrucosis or "lixa" on coconut tree and other palms, facilitating the molecular studies of these biotrophic microorganisms. The method proposed enabled a fast, reproducible and reliable DNA extraction from Camarotella species. Tipo: Info:eu-repo/semantics/article Palavras-chave: Camarotella torrendiella; C. acrocomiae; Verrucosis on coconut; DNA; PCR. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200001 Alterations in Biomarkers Associated with Sterility in Pectinophora gossypiella (Saunders) Induced by Gamma Irradiation BABT Ali,Hanaa Mahmoud; Moustafa,Hemat Zakaria; Sayed,Rehab Mahmoud. ABSTRACT Nowadays, radiation technology is widely used to produce changes in Biosystems. The goal of this work is to determine the variation induced male Pectinophora gossypiella in gamma-irradiated as pupae using 50Gy and 150Gy. Comparing elements composition and DNA (using RAPD-PCR) between substerile 50Gy and the sterile dose 150Gy in P. gossypiella showed variation between them. Potassium (K) was the most abundant elements in unirradiated and irradiated males followed by magnesium (Mg). The percentage of heavy metals as copper, zinc, and cadmium concurrent with K was directly proportional to the radiation dose. While the percentage of Mg, Phosphorous and calcium decreased as the radiation dose increased. The results also revealed that some extra bands... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pectinophora gossypiella; Gamma rays; RAPD-PCR; Elements; DNA. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100401 Plant or fungal sequences? An alternative optimized PCR protocol to avoid ITS (nrDNA) misamplification BABT Miranda,Vitor Fernandes Oliveira de; Martins,Vanderlei Geraldo; Furlan,Antonio; Bacci Jr.,Maurício. The nuclear ribosomal DNA internal transcribed spacers (ITS1 and ITS2) from leaves of Drosera (Droseraceae) were amplified using "universal" primers. The analysis of the products demonstrated most samples were a molecular mixture as a result of unsuccessful and non-specific amplifications. Among the obtained sequences, two were from Basidiomycota fungi. Homologous sequences of Basidiomycota were obtained from GenBank database and added to a data set with sequences from Drosera leaves. Parsimony analysis demonstrated that one sequence was amplified from an Ustilaginomycetes fungus, and another from a Heterobasidiomycetes. Possibly these fungi were associated to leaves of Drosera, and not because of samples contamination. In order to provide optimization and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Polymerase; DNA; Drosera; Fungi; Phylogeny. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100018 Registros recuperados: 189 Primeira ... 1 2 3 4 5 6 7 8 9 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco