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| Tenório, Bruno Mendes; Universidade Federal Rural de Pernambuco; Jimenez, George Chaves; UFRPE; Guerra, Maria Madalena Pessoa; UFRPE; Nogueira, Romildo de Albuquerque; UFRPE; Silva Junior, Valdemiro Amaro da; UFRPE. |
| A exposição da sociedade a Campos Eletromagnéticos (CEM) vem aumentando vertiginosamente em virtude da ampla expansão tecnológica observada nos últimos anos. Tanto a geração, como a distribuição e a utilização de energia elétrica podem gerar Campos Eletromagnéticos de baixa freqüência (50 e 60 Hz). Pesquisas vêm demonstrando que a exposição a estes CEM podem proporcionar alterações fisiológicas significativas, apesar disto, ainda não estão totalmente esclarecidos a extensão destes efeitos, nem os mecanismos de ação que envolve a interação dos CEM com os organismos biológicos. O presente trabalho teve como principal objetivo verificar os efeitos dos CEM (60 Hz e 1 mT) sobre a integridade de DNA e morfologia espermática de ratos sexualmente maduros, que... |
| Tipo: Estudos Morfológicos; Itegridade de DNA |
Palavras-chave: 2.06.00.00-3; 2.07.00.00-8 espermatozoide; Lesões de DNA; Patologias espermáticas Morfologia; Fisiologia electromagnetic wave; Non-ionizing radiation; Spermatozoa; DNA damage; Sperm pathologies. |
| Ano: 2011 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5417 |
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| Picco,Sebastián; Ponzzinibio,M. Virginia; Mattioli,Guillermo; Rosa,Diana; Minatel,Leonardo; Fazzio,Luis; Seoane,Analía. |
| Molybdenosis is a disease caused by the depressing effect of molybdenumn (Mo) on the physiological availability of Copper (Cu). The present study was carried out in order to analyze the ability of Mo to cause damage on the DNA integrity and changes in membrane fatty acids by oxidative damage. Holstein male calves were fed a Mo-supplemented diet for 9 months. Variables evaluated were plasma Cu concentration, erythrocyte Cu content and SOD activity, comet assay and analysis of the fatty acid composition of erythrocyte membranes. The statistical design was a completely randomized with one single factor and two replications. Copper plasma concentration, erythrocyte copper concentration and Cu/Zn SOD activity were analyzed using the t test. Chi-square test was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molybdenum; Copper deficiency; Oxidative damage; DNA damage; Bos taurus; SOD activity. |
| Ano: 2012 |
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952012000200002 |
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| GIOVANELLA,FRANCIANNE; FERREIRA,GABRIELA K.; PRÁ,SAMIRA D.T. DE; CARVALHO-SILVA,MILENA; GOMES,LARA M.; SCAINI,GISELLI; GONÇALVES,RENATA C.; MICHELS,MONIQUE; GALANT,LETÍCIA S.; LONGARETTI,LUIZA M.; DAJORI,ANA LUIZA; ANDRADE,VANESSA M.; DAL-PIZZOL,FELIPE; STRECK,EMILIO L.; SOUZA,RENAN P. DE. |
| Primaquine and chloroquine are used for the treatment of malaria; evidence from the literature suggests that these drugs may induce oxidative stress. In this study we investigated the effects of primaquine and chloroquine on oxidative damage and DNA damage in brain, liver and kidney of rats after 7, 14 and 21 days of administration. Our results demonstrated that primaquine causes DNA damage in brain after 7, 14 and 21 days, and in liver after 7 and 14 days. Moreover, primaquine increases TBARS levels in the kidney and protein carbonyls in the brain after 14 days, and decreases protein carbonyls in the liver after 7 days. Whereas chloroquine causes DNA damage in the kidney after 7 and 14 days, and in the liver after 14 and 21 days, increases TBARS levels in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Primaquine; Chloroquine; Oxidative damage; DNA damage; Antimalarial drugs. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000301487 |
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| MOLZ,PATRÍCIA; ELLWANGER,JOEL H.; ZENKNER,FERNANDA F.; CAMPOS,DEIVIS DE; PRÁ,DANIEL; PUTZKE,MARISA T.L.; FRANKE,SILVIA I.R.. |
| ABSTRACT This study evaluated the recognition memory and the levels of DNA damage (blood and hippocampus) in undernourished young Wistar rats. The experiment was conducted along 14-week with rodents divided in control group (CG, n=8) and undernourished group (UG, n=12) which was submitted to caloric restriction. Nutritional status for undernutrition was defined by Body Mass Index (BMI) ≤0.45g/cm2 and by weighting the organs/tissue (liver, spleen, intestine, peritoneal fat, kidney and encephalon). The Novel Object Recognition Test assessed recognition memory and the Comet Assay evaluated the levels of DNA damage. Student t test, 2-way ANOVA and Pearson's correlation analysis were used and the significance level was of p<0.05. The UG showed lower BMI and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA damage; Memory; Nutritional status; Undernutrition; Wistar rats. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000501863 |
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| FRANKE,SILVIA I.R.; MOLZ,PATRÍCIA; MAI,CAMILA; ELLWANGER,JOEL H.; ZENKNER,FERNANDA F.; HORTA,JORGE A.; PRÁ,DANIEL. |
| ABSTRACT The purpose of this study was to determine the effects of the high consumption of sucrose on the levels of DNA damage in blood, hippocampus and bone marrow of rats. Male Wistar rats were treated for 4 months with sucrose (10% for 60 initial days and 34% for the following 60 days) in drinking water, and then, glycemia and glycated hemoglobin (A1C) were measured. Levels of DNA damage in blood and hippocampus were evaluated by the comet assay. The micronucleus test was used to evaluate chromosomal damages in the bone marrow. The sucrose treatment significantly increased (p<0.01) the serum glucose levels (~20%) and A1C (~60%). The level of primary DNA damage was significantly increased (p<0.05) in hippocampal cells (~60%) but not in peripheral... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Comet assay; Diabetes; DNA damage; Glycated hemoglobin; Micronucleus; Sucrose. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000602657 |
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| FRANKE,SILVIA I.R.; MOLZ,PATRÍCIA; MAI,CAMILA; ELLWANGER,JOEL H.; ZENKNER,FERNANDA F.; HORTA,JORGE A.; PRÁ,DANIEL. |
| ABSTRACT We evaluated the influence of hesperidin and vitamin C (VitC) on glycemic parameters, lipid profile, and DNA damage in male Wistar rats treated with sucrose overload. Rats were divided into six experimental groups: I-water control; II-sucrose control; III-hesperidin control; IV-VitC control; V-co-treatment of sucrose plus hesperidin; VI-co-treatment of sucrose plus VitC. We measured the levels of triglycerides, total cholesterol, HDL-c, LDL-c, fasting glucose, and glycated hemoglobin (A1C). DNA damage was evaluated in blood and brain cells using the comet assay and the micronucleus test was used to evaluate chromosomal damages in the rat bone marrow. Co-treatment with VitC, but not with hesperidin, normalized the serum glucose. No effect of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA damage; Hesperidin; Glycated hemoglobin; Lipid profile; Sucrose; Vitamin C. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502203 |
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| SCHNEIDER,MARCIA R.; SILVA,ANDRÉA L.G. DA; GOULART,CÁSSIA L.; SCHNEIDERS,PALOMA B.; WEBER,AUGUSTO F.; POSSUELO,LIA G.; VALIM,ANDREIA R.M.. |
| Abstract: This study evaluated 24 patients with lung cancer (CA) and 23 individuals with no smoking history or cancer in the family and without respiratory disease in childhood (CO). Peripheral blood lymphocytes was used to perform alkaline comet assay and to assess DNA damage as well as to evaluate methyl methane sulfonate (MMS) DNA repair after one hour and three hours at 37 ºC. The percentage of residual damage (RD) after three hours of MMS treatment, for each patient was assessed. The majority of patients were in the CA group, male patients, former smokers, with a history of smoking for 15 years and without associated comorbidities. Alkaline and residual damages were higher in the CA group when compared to controls (alkaline damage P = 0.015 and RD P =... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Comet assay; DNA damage; DNA repair; Lung cancer. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500806 |
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| STERPONE,SILVIA; CORNETTA,TOMMASO; ANGIONI,ADRIANO; FISCARELLI,ERSILIA; LUCIDI,VINCENZINA; TESTA,ANTONELLA; COZZI,RENATA. |
| Cystic Fibrosis (CF) is an autosomal recessive multisystemic disorder showing a highly heterogeneous phenotype, even among siblings carrying identical CFTR mutations. Moreover, oxidative stress is of central importance in the pathogenesis of cystic fibrosis. The present study seeks to value the presence of oxidative damage in CF patients and the possible modifier effect of repair and glutathione-S-transferase genes. We analysed the presence of DNA damage in leukocytes of 63 CF patients at an Italian CF centre and 63 controls, through the alkaline Comet assay to detect DNA strand breaks. Furthermore, controls and 93 CF subjects were genotyped for 5 genes by RFLP-PCR (XRCC1,0GG1,GSTP1) and PCR assay (GSTM1, GSTT1). No difference in Comet assay values was... |
| Tipo: Journal article |
Palavras-chave: Comet assay; Cystic fibrosis; DNA damage; Genetic polymorphisms; Modifier genes. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400009 |
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| Faisal,Mohammad; Saquib,Quaiser; Alatar,Abdulrahman A.; Al-Khedhairy,Abdulaziz A.; Ahmed,Mukhtar; Ansari,Sabiha M.; Alwathnani,Hend A.; Dwivedi,Sourabh; Musarrat,Javed; Praveen,Shelly. |
| BACKGROUND: Despite manifold benefits of nanoparticles (NPs), less information on the risks of NPs to human health and environment has been studied. Cobalt oxide nanoparticles (Co3O4-NPs) have been reported to cause toxicity in several organisms. In this study, we have investigated the role of Co3O4-NPs in inducing phytotoxicity, cellular DNA damage and apoptosis in eggplant (Solanum melongena L. cv. Violetta lunga 2). To the best of our knowledge, this is the first report on Co3O4-NPs showing phytotoxicity in eggplant. RESULTS: The data revealed that eggplant seeds treated with Co3O4-NPs for 2 h at a concentration of 1.0 mg/ml retarded root length by 81.5 % upon 7 days incubation in a moist chamber. Ultrastructural analysis by transmission electron... |
| Tipo: Journal article |
Palavras-chave: Cobalt oxide nanoparticles; Nanotoxicity; DNA damage; Apoptosis; Oxidative stress. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100020 |
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| Votto,Ana P. S; Domingues,Beatriz S; de Souza,Michele M; da Silva Júnior,Flavio M. R; Caldas,Sergiane S; Filgueira,Daza M. V. B; Clementin,Rosilene M; Primel,Ednei G; Vallochi,Adriana L; Furlong,Eliana B; Trindade,Gilma S. |
| Onion (Allium cepa) is being studied as a potential anticancer agent, but little is known regarding its effect in multidrug resistance (MDR) cells. In this work, the cytotoxicity of crude onion extract (OE) and fractioned extract (aqueous, methanolic and ethyl acetate), as well as some onion compounds (quercetin and propyl disulfide) were evaluated in Lucena MDR human erythroleukemic and its K562 parental cell line. The capacity of OE to induce apoptosis and/or necrosis in these cells, the possible participation of oxidative stress and DNA damage were also assessed. Similar sensitivities were obtained for both tumoral cells, however only OE caused significant effects in the cells. In K562 cells, a significant increase of apoptosis was verified while the... |
| Tipo: Journal article |
Palavras-chave: Antioxidant; Apoptosis and/or necrosis; DNA damage; MDR phenotype; Onion (Allium cepa); Tumoral cell. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400007 |
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| Singh,Thangjam Davis; Meitei,Heikrujam Thoihen; Sharma,Adhikarimayum Lakhikumar; Robinson,Asem; Singh,Lisam Shanjukumar; Singh,Thiyam Ramsing. |
| BACKGROUND: Clinical use of chemotherapeutic drug, cisplatin is limited by its toxicity and drug resistance. Therefore, efforts continue for the discovery of novel combination therapies with cisplatin, to increase efficacy and reduce its toxicity. Here, we screened 16 medicinal plant extracts from Northeast part of India and found that leaf extract of Zanthoxylum armatum DC. (ZALE) induced cytotoxicity as well as an effect on the increasing of the efficiency of chemotherapeutic drugs (cisplatin, mitomycin C and camptothecin). This work shows detail molecular mechanism of anti-cancer activity of ZALE and its potential for combined treatment regimens to enhance the apoptotic response of chemotherapeutic drugs. RESULTS: ZALE induced cytotoxicity, nuclear... |
| Tipo: Journal article |
Palavras-chave: Zanthoxylum armatum; Apoptosis; ERK activation; Cisplatin sensitization; DNA damage. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100046 |
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| Bouriche,Hamama; Kada,Seoussen; Senator,Abderrahmane; demirtas,Ibrahim; Ozen,Tevfik; Toptanci,Bircan Çeken; Kizil,Goksel; Kizil,Murat. |
| ABSTRACT The protective activity of methanolic (Met E) and aqueous (Aq E) extracts of Globularia alypum L. (G. alypum) against DNA, lipid and protein oxidative damage was investigated. Moreover, the scavenging, chelating, and reducing power activities of the extracts were also evaluated. Phytochemical analysis was performed to determine phenolic compounds. Results showed that Met E and Aq E were rich in phenolic compounds, and were able to scavenge DPPH˙ with IC50 values of 48.61 µg/mL and 51.97 µg/mL, respectively. In addition, both extracts were able to chelate ferrous ions. At 300 μg/mL, the chelating activity was 97.53% and 91.02%, respectively. The reducing power of these extracts was also remarkable and concentration dependent. At 100 µg/mL, both... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidants; DNA damage; Globularia alypum; Lipid peroxidation; Protein oxidation. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100422 |
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| Pandir,Dilek. |
| ABSTRACTThe aim of this work was to assess the damage of DNA in human blood cell and spermin vitro under the influence of furan. These cells were administered 0-600 μM of furan at 37 and 32oC for 30 and 60 min, respectively. A significant increase in tail DNA%, tail length and moment indicating DNA damage was observed at increasing doses when compared to the controls. The treatment with 300 and 600 μM of furan showed a maximum increase of 86.74 ± 2.43 and 93.29 ± 8.68 compared to the control tail DNA% in lymphocytes. However, only 600 μM of furan showed a maximum increase of 94.71 ± 6.24 compared to the control tail DNA% in sperm. The results suggested that furan caused DNA damage in lymphocytes at increasing doses, but appeared to have not the same effect... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Furan; Tail length; DNA damage; Human blood cells; Comet assay. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500773 |
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| Pandir,Dilek. |
| ABSTRACT Organophosphorous pesticides (OPs) posses a great potential of acute toxicity for exposed animals and men. To evaluate the toxic potential of the organophosphate diazinon on root meristematic cells of Allium cepa L., was created two groups: In group 1 (control group), was not given any chemical. In group 2 (diazinon-treatment group), different doses (10, 40, 80 and 160 ppm) and times periods (24, 48 and 72 h) were administered. After exposure, cell death, effective concentration (EC50), mitotic index, cellular /chromosome aberrations, DNA damage by comet assay and RAPD-PCR were assessed at exposure times. EC50 value of diazinon was detected approximately 80 ppm. Hyperchromasia, later segragation, micronucleus, pulverised nucleus, nuclear... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diazinon; Allium test; DNA damage; Genotoxicty; RAPD-PCR. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100406 |
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| Xu,Xianrong; Lu,Jing; Sheng,Hongqiang; Zhang,Long; Gan,Tieer; Zhang,Jianyun; Xu,Yuying; Zhu,Xinqiang; Yang,Jun. |
| The melamine and cyanuric acid (CA) complex has been suggested to cause the toxic effects observed in melamine-contaminated food or milk. However, the cytotoxic and genotoxic effects of co-exposure to melamine and CA are not fully clear. Therefore, the cytotoxic effects of melamine and CA were first examined by co‐exposure in human kidney 293 cells using the MTT assay. During a 24-h period for the three concentrations tested (0.5, 1, and 5 mg/mL), neither melamine nor CA alone showed significant toxic effects on 293 cells at 0.5 mg/mL, while higher concentrations led to decreased in cell viability. However, co-exposure to several combinations of melamine and CA [100:1, 10:1, 1:10, and 1:100 (v:v), at a final concentration of 0.5 mg/mL] did cause... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Melamine; Cyanuric acid; ΓH2AX; DNA damage; Genotoxicity. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500612 |
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| Tian,Meili; Yang,Shuang; Yan,Xinpeng. |
| Icariin has been reported to possess high anticancer activity. Colon carcinoma is one of the leading causes of cancer-related mortality worldwide. Here, the anticancer activity of icariin against HCT116 colon carcinoma cells and the possible underlying mechanism were studied. The trypan blue staining assay, wound healing assay, clonogenic assay, CCK-8 assay, and Annexin V-FITC/PI double staining method were carried out to determine the changes of HCT116 cell growth and migration. mRNA and protein expressions were determined by quantitative real-time PCR and western blot, respectively. Moreover, small interfering RNA (siRNA) plasmid was used to examine the role of p53 in icariin-induced apoptosis in HCT116 cells. Icariin significantly suppressed colon... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Colon carcinoma; Icariin; Mechanism; P53; DNA damage; Caspases. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000605 |
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| Registros recuperados: 49 | |
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