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| MASETTO,TATHIANA E.; FARIA,JOSE M.; FRAIZ,ANA C.R.. |
| This work aimed to characterize the re-induction of desiccation tolerance (DT) in germinated seeds, using polyethylene glycol (PEG 8000). Cell changes were investigated through cytological assays (cell viability and transmission electronic microscopy) as well as DNA integrity during loss and re-establishment of DT. The loss of DT was characterized by drying germinated seeds with different radicle lengths (1, 2, 3, 4 and 5 mm) in silica gel, decreasing the moisture content to ten percentage points intervals, followed by pre-humidification (100% RH / 24 h) and rehydration. To re-induce DT, germinated seeds were treated for 72 h with PEG (-2.04 MPa) and PEG (-2.04 MPa) + ABA (100 µM) before dehydration. Germinated seeds did not tolerate desiccation to 10%... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytological alterations; DNA integrity; Seedlings; Moisture content. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000301273 |
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| Carneiro-Leite,Laicia; Bashiyo-Silva,Cristiane; Oliveira,Yasmim Alves Araújo; Borges,Laís Pedroso; Sanchez,Malbelys Padilla; Silva,Luciane Gomes da; Lobato,Stella Indira Rocha; Rodrigues-Lisoni,Flávia Cristina; Veríssimo-Silveira,Rosicleire; Ninhaus-Silveira,Alexandre. |
| Abstract This study aimed to determine the semen characteristics of Astyanax lacustris after hormonal induction and to evaluate the sensitivity of the species sperm to cryoprotective solutions based on the cryoprotectants dimethyl sulfoxide and methyl glycol. Volume, color, sperm concentration, total motility and aspects of sperm movement were analyzed using “Integrated Semen Analysis System”. Three different extenders were tested: A) glucose 5%+egg yolk 10%, B) BTS®5% and C) glucose 5% and two permeable cryoprotectants: dimethyl sulfoxide (Me2SO) and methyl glycol (MTG). Fresh A. lacustris semen presented total motility of 76.6±11.2%, motility duration of 33.0±2.2s, sperm concentration of 7.22±3.2×109sptz/mL and seminal osmolality of 219±0.03mOsm/kg-1.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Characiformes; DNA integrity; Freezing; Semen quality; Yellow-tailed Lambari. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252020000300214 |
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