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| SanJuan-Badillo,Andrea; Galvez,Amanda; Plasencia,Javier; Quirasco,Maricarmen. |
| In Mexico, regulations for growing genetically modified (GM) maize (Zea mays L.) plants have been enforced in order to prevent gene flow to native landraces and wild relatives. Field surveys are necessary and Mexican government agencies have the mandate to perform them. Because of their specificity, PCR-based methods are suitable for field monitoring of GM organisms but it is necessary to assess their performance, since they are greatly influenced by the DNA preparation quality inherent to the extraction method. In this study, genomic DNA was extracted from various maize tissues (e.g. pollen, leaves, spikelets and grains) using five different commercial purification protocols. DNA quality was analyzed spectrophotometrically, by gel electrophoresis, and as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: PCR inhibition; GM maize; Variability of DNA yield; DNA quantification; GMO detection. |
| Ano: 2014 |
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952014000100002 |
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| Vora,Shruti Dineshbhai; Kumar,Vineet; Asodiya,Foram Arvindbhai; Singh,Vivek Kumar; Fefar,Dhaval Tribhovanbhai; Gajera,Harsukh Popatbhai. |
| Abstract The purpose of the study was to develop a xenogenic bubaline diaphragm matrix (BDM) for abdominal hernia repair. A fresh diaphragm was decellularized using aqueous sodium dodecyl sulfate (SDS) solutions (0.5-4% w/v) over a period. Acellularity was confirmed histologically and characterized by Masson’s trichrome staining, scanning electron microscopy (SEM), DNA quantification, agarose gel electrophoresis, and Fourier-transform infrared spectroscopy. The BDM was used for clinical abdominal hernia repair in six cattle. Clinical, hemato-biochemical and antioxidant parameters were evaluated to assess biocompatibility of xenogenic BDM. Histologically, the diaphragm treated with 2% SDS for 48 h showed complete acellularity and orderly arranged collagen... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biocompatibility; Bubaline diaphragm matrix; DNA quantification; Spectroscopy; Scanning electron microscopy. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100309 |
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