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| Cordeiro,Everson Willian Fialho; Hilgert,Renata Medeiros; Batista,Luiz Alcides das Chagas; Souza,Raul Oliveira; Oliveira,Luís Flávio Souza de; Machado,Michel Mansur; Malesuik,Marcelo Donadel; Paula,Fávero Reisdorfer; Stopiglia,Cheila Denise Ottonelli; Paim,Clésio Soldateli. |
| Danofloxacin is a veterinary fluoroquinolone used to treat respiratory and gastrointestinal diseases of birds, pigs and cattle. The literature reviewed shows some analytical methods to quantify this fluoroquinolone, but microbiological and biological safety studies are limited. The analytical methods were validated by the Official Codes. The LC-DAD method was developed and validated using an RP-18 column, mobile phase containing a mixture of 0.3% triethylamine (pH 3.0) and acetonitrile (85:15, v/v). The microbiological assay was performed by agar diffusion method (3 x 3) and Staphylococcus epidermidis as a microorganism test. Forced degradation studies were performed in both methods. The minimum inhibitory concentration (MIC) was performed by test... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Danofloxacin; Microbiological assay; Biological safety; In silico toxicology; Fluoroquinolones photodegradation. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100555 |
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| NIU,Rui; YANG,Yamei; WANG,Yadan; LUO,Shilong; ZHANG,Cheng; WANG,Yun. |
| Abstract In this study, an immunoaffinity column was prepared by covalently coupling of polyclonal antibody against danofloxacin (DAN) with chitosan microparticles and its potential application to selective extraction of DAN from food samples was also evaluated. The produced antibody exhibited high sensitivity, with an IC50 value of 4.56 ng/mL towards DAN, and good specificity, with less than 0.5% of cross-reactivity towards DAN structurally related compounds. The developed immunoaffinity column had an approximate binding capacity of 2300 ng/mL gel and a relative standard deviation (RSD) of 3.7% among columns. The immunoaffinity columns were then challenged with DAN-fortified milk samples, recoveries of DAN were found to be in the range of 83.8% to 98.8%,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Danofloxacin; Immunoaffinity chromatography; Clean-up; Residual detection. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000200500 |
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