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| Pereira,V.A.; Auler Jr.,J.O.; Carmona,M.J.; Mateus,F.H.; Lanchote,V.L.; Breimer,D.D.; Santos,S.R.C.J.. |
| We describe a new simple, selective and sensitive micromethod based on HPLC and fluorescence detection to measure debrisoquine (D) and 4-hydroxydebrisoquine (4-OHD) in urine for the investigation of xenobiotic metabolism by debrisoquine hydroxylase (CYP2D6). Four hundred µl of urine was required for the analysis of D and 4-OHD. Peaks were eluted at 8.3 min (4-OHD), 14.0 min (D) and 16.6 min for the internal standard, metoprolol (20 µg/ml). The 5-µm CN-reverse-phase column (Shimpack, 250 x 4.6 mm) was eluted with a mobile phase consisting of 0.25 M acetate buffer, pH 5.0, and acetonitrile (9:1, v/v) at 0.7 ml/min with detection at lexcitation = 210 nm and lemission = 290 nm. The method, validated on the basis of measurements of spiked urine, presented 3... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Debrisoquine; 4-hydroxydebrisoquine; HPLC-F; Urinary excretion kinetics; Debrisoquine test validation; CYP2D6. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500004 |
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