Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados BJMBR (1) Electron. J. Biotechnol. (1) Autor Bassi,C.L. (1) Cipolotti,R. (1) Defávery,R. (1) Martelli,L. (1) Menéndez-González,Manuel (1) Pérez-Pinera,Pablo (1) Scrideli,C.A. (1) Tone,L.G. (1) Vega,José Antonio (1) Mais... Palavra-chave Deletion (2) Mutagenesis (1) Mutation (1) Neuroblastoma (1) P15 gene (1) P16 gene (1) Plasmid (1) Polymerase chain reaction (1) Mais... Tipo do documento Info:eu-repo/semantics/other (1) Journal article (1) Ano 2006 (1) 2004 (1) País Brazil (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Deletion of DNA sequences of using a polymerase chain reaction based approach Electron. J. Biotechnol. Pérez-Pinera,Pablo; Menéndez-González,Manuel; Vega,José Antonio. We developed a simple, rapid and reliable method to delete DNA fragments in plasmids using a polymerase chain reaction based amplification of the circular DNA sequence that excludes the fragment to be deleted. The primers are designed to contain a non-complementary 5' sequence consisting of a restriction enzyme target sequence. Following PCR amplification, the plasmid is digested with Dpn I to eliminate the template DNA, with the chosen restriction enzyme, and ligated. The only limitation is the selection of the restriction enzyme target sequence that must not be present in the original plasmid. The method is straightforward in its execution and success relies on a meticulous primer design that permits us obtain 100% of transformants containing the desired... Tipo: Journal article Palavras-chave: Deletion; Mutagenesis; Polymerase chain reaction; Plasmid. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500018 Lack of evidence for mutations or deletions in the CDKN2A/p16 and CDKN2B/p15 genes of Brazilian neuroblastoma patients BJMBR Bassi,C.L.; Martelli,L.; Cipolotti,R.; Scrideli,C.A.; Defávery,R.; Tone,L.G.. Neuroblastoma, the most common extracranial tumor in childhood, has a wide spectrum of clinical and biological features. The loss of heterozygosity within the 9p21 region has been reported as a prognostic factor. Two tumor suppressor genes located in this region, the CDKN2B/p15 and CDKN2A/p16 (cyclin-dependent kinase inhibitors 2B and 2A, respectively) genes, play a critical role in cell cycle progression and are considered to be targets for tumor inactivation. We analyzed CDKN2B/p15 and CDKN2A/p16 gene alterations in 11 patients, who ranged in age from 4 months to 13 years (male/female ratio was 1.2:1). The most frequent stage of the tumor was stage IV (50%), followed by stages II and III (20%) and stage I (10%). The samples were submitted to the... Tipo: Info:eu-repo/semantics/other Palavras-chave: P15 gene; P16 gene; Deletion; Mutation; Neuroblastoma. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100014 Registros recuperados: 2 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco