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The use of Proteinase K to access genitalia morphology, vouchering and DNA extraction in minute wasps Anais da ABC (AABC)
MARTINELLI,ARTURO B.; WAICHERT,CECILIA; BARBOSA,DIEGO N.; FAGUNDES,VALÉRIA; AZEVEDO,CELSO O..
ABSTRACT Genitalia are rich source of characters in insect taxonomy. Usually, they are examined after dissection and cleaning with potassium hydroxide (KOH), procedure that may damage both genital morphological structures and intracellular molecular contents. Enzymatic procedure with Proteinase K has been used to clean muscle off the genitalia while extract DNA, but its damage to the genital structures has not been evaluated. Herein, we qualitatively compare the use of KOH and Proteinase K to prepare genital structures in minute insects (Hymenoptera: Bethylidae). We show that Proteinase K is better to preserve the genital structure and provides quality DNA.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diaphanization; Genitalia preparation; KOH; Wolbachia.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000401629
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Leaf architecture of Rubiaceae Juss. from caatinga vegetation in Brazil Biota Neotropica
Lima,Mirella Priscila de Souza; Soares,Adriana; Sousa,José Lucas Ribeiro de; Carvalho,Márcia Santos; Porto,Jorge Marcelo Padovani; Braga,Francyane Tavares.
Abstract: The study and characterization of leaf venation plays a key role in the recognition of taxonomic groups that have been identified mainly based on reproductive traits. This study aimed at characterizing the pattern of leaf venation of Rubiaceae, recognizing individual characters among the taxa of this group and testing the use of leaf architecture as a taxonomic tool capable of providing vegetative and diagnostically characteristics between species. Leaves of 14 species distributed in eight genera from Rubiaceae were diaphanized and classified. The study area is located in Área de Proteção Ambiental Serra Branca/Raso da Catarina, Bahia State, Brazil. The characterization of secondary veins, including type, spacing and number of pairs, third...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diaphanization; Leaf veining; Raso da Catarina; Semiarid.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032019000100203
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Diaphanization: A Standardized Protocol for Non-Fetal Tissue Preservation International Journal of Morphology
Rueda-Esteban,Roberto Javier; Palacio Varona,Juliana; López-McCormick,Juan Sebastián; Hernández Restrepo,Juan David.
In response to the arising difficulty of dissection use during anatomy courses, medical schools have been forced to research alternative teaching methods. These are meant to help students develop three dimensional mental images of the human body and increase spatial reasoning, thus improving the learning process of human morphology. One of those methods, used at the Universidad de los Andes is the Diaphanization process. This technique is a well-known method for specimen preservation, used as an anatomy research and teaching tool. Even though it is frequently used, finding a standardized protocol in indexed journals is not possible, which hinders the use of the technique. The standardization of an updated protocol is a need in order to continue exploiting...
Tipo: Journal article Palavras-chave: Teaching of anatomy; Diaphanization; Preservation of Adult Tissue; Standardized protocol.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200027
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