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Identification of genes differentially expressed in hyphae of Candida albicans BJM
Melo,Analy Salles de Azevedo; Serafim,Rui Cosme; Briones,Marcelo Ribeiro da Silva.
The ability to switch from yeast to hyphal forms is essential for Candida albicans virulence. This morphological switch involves the expression of hyphal-specific genes under the control of transcriptional factors. To contribute to the discovery of hyphal-specific genes, we used a differential screening method where clones of a genomic DNA library were hybridized with yeast and hyphal cDNA probes. Two clones with increased expression in hyphae were selected for study. Sequencing these clones, we found that they encoded two important metabolic genes, CaHXT7 (high-affinity hexose transporter) and CaYLL34 (member of the AAA ATPase family). CaHXT7 and CaYLL34 ORFs were completely determined. Analyses of the putative proteins show that: (1) CaHxt7p has one...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Differential screening; Candida albicans; HXT7; YLL34.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500046
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