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| Fietto,Luciano G.; Costa,Maximiller D.L.; Cruz,Cosme D.; Souza,Alessandra A.; Machado,Marcos A.; Fontes,Elizabeth P.B.. |
| The completion of the genome sequencing of the Arabidopsis thaliana model system provided a powerful molecular tool for comparative analysis of gene families present in the genome of economically relevant plant species. In this investigation, we used the sequences of the Arabidopsis Hsp70 gene family to identify and annotate the Citrus Hsp70 genes represented in the CitEST database. Based on sequence comparison analysis, we identified 18 clusters that were further divided into 5 subgroups encoding four mitochondrial mtHsp70s, three plastid csHsp70s, one ER luminal Hsp70 BiP, two HSP110/SSE-related proteins and eight cytosolic Hsp/Hsc70s. We also analyzed the expression profile by digital Northern of each Hsp70 transcript in different organs and in response... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chaperones; Digital northern; Heat shock proteins; Genome; Expressed sequence tags. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500017 |
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| Reis,Marcelo S.; Takita,Marco A.; Palmieri,Darío A.; Machado,Marcos A.. |
| In this work we describe all the computational environments, pipelines, and web services developed for the CitEST transcriptome project, on which all the annotation researchers relied. We also present a complete list of CitEST libraries and, for each of them, the general features after the in silico processing, showing some quantitative information. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Transcriptomics; Comparative genomics; Clustering; Trimming; Digital northern. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500031 |
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