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Disector Z-axis mechanical method for stereology Anais da ABC (AABC)
Xavier-Vidal,Ricardo.
The purpose of this work is to calibrate a not expensive microscope to be applied in Optical Disector estimation. The evaluation of "Z-axis" bias and the "Z-axis" calibration were made utilizing a cover slip and a "manual digimatic outside micrometer scale" (Mitutoyo, Japan). Calibrating the cover slip we performed the calibration of the "Z-axis" of a microscope. In the cover slip two lines were painted with different colors using a pen glass. A blue line was painted on the up surface and another line (red) on the bottom surface of the cover slip forming a cross. Two metal rings with one palette welded in each were adapted in the microscope. Other palette was welded in the gross focuses in order to restrict the route of the fine focuses (Z-axis study)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stereology; Disector; Optical disector; Morphometry; Z-axis.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000200028
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Stereological tools in biomedical research Anais da ABC (AABC)
Mandarim-de-Lacerda,Carlos A..
Stereological studies are more and more frequent in literature, particularly in the development/evolution, pathology, and neurosciences areas. The stereology challenge is to understand the structural inner threedimensional arrangement based on the analysis of the structure slices only showing two-dimensional information. Cavalieri and Scherle's methods to estimate volume, and Buffon's needle problem, are commented in the stereological context. A group of actions is needed to appropriately quantify morphological structures (unbiased and reproducibly), e.g. sampling, isotropic and uniform randomly sections (Delesse's principle), and updated stereological tools (disector, fractionator, nucleator, etc). Through the correct stereology use, a quantitative study...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Morphometry; Stereology; Volume density; Surface density; Length density; Disector; Fractionator.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652003000400006
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