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Characteristics of dr1790 disruptant and its functional analysis in Deinococcus radiodurans BJM
Cheng,Jianhui; Wang,Hu; Xu,Xin; Wang,Liangyan; Tian,Bing; Hua,Yuejin.
Deinococcus radiodurans (DR) is an extremophile that is well known for its resistance to radiation, oxidants and desiccation. The gene dr1790 of D. radiodurans was predicted to encode a yellow-related protein. The primary objective of the present study was to characterize the biological function of the DR1790 protein, which is a member of the ancient yellow/major royal jelly (MRJ) protein family, in prokaryotes. Fluorescence labeling demonstrated that the yellow-related protein encoded by dr1790 is a membrane protein. The deletion of the dr1790 gene decreased the cell growth rate and sensitivity to hydrogen peroxide and radiation and increased the membrane permeability of D. radiodurans. Transcript profiling by microarray and RT-PCR analyses of the dr1790...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Deinococcus radiodurans; Dr1790 disruptant; Characteristics; Functional analysis.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200601
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