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	Provedor de dados BJMBR (1) Autor Lovrien,R.E. (1) Mares-Guia,M. (1) Santoro,M.M. (1) Schneedorf,J.M. (1) Palavra-chave Binding isotherm (1) Coprecipitation (1) Dye binding (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2001 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última A time-dependent, two-step binding mode of the nitro dye flavianic acid to trypsin in acid media BJMBR Schneedorf,J.M.; Santoro,M.M.; Lovrien,R.E.; Mares-Guia,M.. Synthetic dyes bind to proteins causing selective coprecipitation of the complexes in acid aqueous solution by a process of reversible denaturation that can be used as an alternative method for protein fractionation. The events that occur before precipitation were investigated by equilibrium dialysis using bovine trypsin and flavianic acid as a model able to cause coprecipitation. A two-step mode of interaction was found to be dependent on the incubation periods allowed for binding, with pronounced binding occurring after 42 h of incubation. The first step seems to involve hydration effects and conformational changes induced by binding of the first dye molecule, following rapid denaturation due to the binding of six additional flavianate anions to the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Dye binding; Binding isotherm; Coprecipitation. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800012 Registros recuperados: 1 Primeira ... 1 ... Última

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