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Evaluation of alveolar macrophage function after experimental infection with equine herpesvirus-1 in horses Arq. Bras. Med. Vet. Zootec.
Mori,E.; Mori,C.M.C.; Della Libera,A.M.M.P.; Lara,M.C.C.S.H.; Fernandes,W.R..
The role of the pulmonary alveolar macrophages (PAM) in the lung defense mechanism was evaluated in horses infected with equine hespesvirus-1 (EHV-1). Five adult horses were exposed to 10(6.6) TCID50 EHV-1 by intranasal instillation. Cytology of bronchoalveolar lavage (BAL) was performed using cytocentrifugation of samples and slides stained by Rosenfeld. Cell concentration was adjusted to 2<FONT FACE=Symbol>´</FONT>10(6) cells/ml, for the measurement of macrophage activity - spreading, phagocytosis of zymosan particles and release of hydrogen peroxide (H2O2). All animals were positive in virus isolation on the second, third and fifth days post-inoculation (DPI). Seroconversion was observed on the 14th DPI. Lymphocytosis was observed by BAL...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Equine; Alveolar macrophage; Equine herpesvirus; EHV-1; Bronchoalveolar lavage.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300005
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Detection of equine herpesvirus 1 DNA in a single embryo and in horse semen by polymerase chain reaction Arq. Bras. Med. Vet. Zootec.
Carvalho,R.; Passos,L.M.F.; Oliveira,A.M.; Henry,M.; Martins,A.S..
The genome of one equine embryo and three equine semen specimens collected from a Brazilian farm were tested by polymerase chain reaction (PCR) for the presence of EHV-1 and EHV-4-specific timidine kinase (TK) sequences. The PCR detected specific EHV-1 TK gene sequences in all samples tested. The peripheral blood leukocytes (PBL) of the embryo donor mare also was amplified by EHV-1 TK primers. Infectious virus was not recovered from any specimens. The animals did not show any clinical signal of EHV-1 or EHV-4 infections. EHV-4 was not detected in the studied specimens. The results indicate that PCR was more sensitive than virus isolation in cell culture for detecting EHV-1 in semen of carrier horses.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Equine; Semen; Embryo; EHV-1; EHV-4; PCR.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400002
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In vitro activity of six antiviral drugs against equid alphaherpesvirus type 1 indicates ganciclovir as promising drug for in vivo studies Ciência Rural
Oliveira,Amanda Lovato de; Cargnelutti,Juliana Felipetto; Mortari,Ana Paula Gnocato; Flores,Eduardo Furtado; Weiblen,Rudi.
ABSTRACT: Equid alphaherpesvirus type 1 (EHV-1) is distributed worldwide and is a major agent of abortion, respiratory and neurological disease in horses. No specific treatment is available for EHV-1 infection, yet the potential of antiviral therapy has been explored. In this study we investigated the in vitro activity of Acyclovir, Ganciclovir, Foscarnet, Famciclovir, Vidarabina and Cidofovir against EHV-1. For this, the MTT test was performed, in which all the tested drugs showed no toxicity up to 200μg/mL. Subsequently, different drug concentrations were submitted to viral plaque reduction assays in cell culture. The selectivity index (SI) of the compounds was determined using the cytotoxic concentration for 50% of cells (CC50), obtained by MTT, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: EHV-1; Plaque assay; Acyclovir; Ganciclovir; Foscarnet; Famciclovir; Vidarabine; Cidofovir.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001200450
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