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| Quaedvlieg, W.; Groenewald, J.Z.; Jesús Yáñez-Morales, M. de; Crous, P.W.. |
| The EU 7th Framework Program provided funds for Quarantine Barcoding of Life (QBOL) to develop a quick, reliable and accurate DNA barcode-based diagnostic tool for selected species on the European and Mediterranean Plant Protection Organization (EPPO) A1/A2 quarantine lists. Seven nuclear genomic loci were evaluated to determine those best suited for identifying species of Mycosphaerella and/or its associated anamorphs. These genes included β-tubulin (Btub), internal transcribed spacer regions of the nrDNA operon (ITS), 28S nrDNA (LSU), Actin (Act), Calmodulin (Cal), Translation elongation factor 1-alpha (EF-1α) and RNA polymerase II second largest subunit (RPB2). Loci were tested on their Kimura-2-parameter-based inter- and intraspecific variation, PCR... |
| Tipo: Article / Letter to the editor |
Palavras-chave: EPPO; Lecanosticta; Q-bank; QBOL. |
| Ano: 2012 |
URL: http://www.repository.naturalis.nl/record/531831 |
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