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| Maluta,Renato Pariz; Stella,Ariel Eurides; Riccardi,Kátia; Rigobelo,Everlon Cid; Marin,José Moacir; Carvalho,Marileda Bonafim; Ávila,Fernando Antonio de. |
| Pathogenic strains of Escherichia coli are the most common bacteria associated with urinary tract infections in both humans and companion animals. Standard biochemical tests may be useful in demonstrating detailed phenotypical characteristics of these strains. Thirteen strains of E. coli isolated from dogs with UTIs were submitted to biochemical tests, serotyping for O and H antigens and antimicrobial resistance testing. Furthermore, the presence of papC, sfa, and afa genes was evaluated by PCR, and genetic relationships were established using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). The antimicrobial that showed the highest resistance rate among the isolates was nalidixic acid (76.9%), followed by cephalotin (69.2%),... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biomarkers; Biotyping; ERIC-PCR; UPEC. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100045 |
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| Moreno,Luisa Zanolli; Castilla,Karina Salvagni; Gobbi,Débora Dirani Sena de; Coutinho,Tania Alen; Ferreira,Thais Sebastiana Porfida; Moreno,Andrea Micke. |
| Haemophilus parasuis infection, known as Glässer's disease, is characterized by fibrinous polyserositis, arthritis and meningitis in piglets. Although traditional diagnosis is based on herd history, clinical signs, bacterial isolation and serotyping, the molecular-based methods are alternatives for species-specific tests and epidemiologic study. The aim of this study was to characterize H. parasuis strains isolated from different states of Brazil by serotyping, PCR and ERIC-PCR. Serotyping revealed serovar 4 as the most prevalent (24 %), followed by serovars 14 (14 %), 5 (12 %), 13 (8 %) and 2 (2 %), whereas 40 % of the strains were considered as non-typeable. From 50 strains tested 43 (86%) were positive to Group 1 vtaA gene that have been related to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Haemophilus parasuis; Glässer disease; Genotyping; ERIC-PCR. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400025 |
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| Bakhshi,Bita; Afshari,Nasim; Fallah,Fatemeh. |
| Abstract Background Shigellosis remains a serious public health problem and an important cause of morbidity and mortality worldwide. The aim of this study was to characterize fliC and the genetic relatedness of Shigella spp. isolated during a one-year period from children in a suspected outbreak in Tehran, Iran. Methods and results Fifty Shigella spp. were isolated from 3779 stool samples of children with diarrhea (prevalence rate: 1.32%). Among the isolates, 92% were characterized as Shigella sonnei, while 6% and 2% were identified as S. flexneri and S. boydii, respectively. S. dysenteriae was not recovered from the patients. All isolates were negative for fliC except for Shigella standard strains. The enterobacterial repetitive intergenic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Shigella spp; ERIC-PCR; FliC; Outbreak. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300529 |
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| Caleffi-Ferracioli,Katiany Rizzieri; Baldin,Vanessa Pietrowski; Noguti,Érika Noda; Siqueira,Vera Lucia Dias; Scodro,Regiane Bertin Lima; Pavan,Fernando Rogério; Hirata,Mario Hiroyuki; Cardoso,Rosilene Fressatti. |
| Tuberculosis (TB) is an infectious disease in which the molecular typing methods allow to have important information about the dynamics of transmission and to assist properly in disease control. Although the ERIC-PCR (Enterobacterial repetitive intergenic consensus-PCR) assay is fast and easy to perform, scarce studies have reported its use in epidemiological studies in TB outbreaks. In this study, we aimed to genotype Mycobacterium tuberculosis and M. bovis isolates by ERIC-PCR and compare its discriminatory power with two other classically used methods: 12 loci-MIRU (Mycobacterial Interspersed Repetitive Units) and Spoligotyping. The M. tuberculosis isolates studied were from northwestern and southwestern and M. bovis from northwestern Parana, Brazil.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tuberculosis; Molecular characterization; ERIC-PCR; Epidemiology. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300616 |
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| Ramos,Carolina Pantuzza; Xavier,Rafael Gariglio Clark; Leal,Carlos Augusto Gomes; Facury Filho,Elias Jorge; Carvalho,Antonio Ultimo de; Viegas,Flávia Mello; Pires,Isadora Honorato; Lopes,Emily Oliveira; Lobato,Francisco Carlos Faria; Silva,Rodrigo Otávio Silveira. |
| ABSTRACT: The present study aimed to describe and characterize, for the first time, two outbreaks of salmonellosis caused by Salmonella Ndolo in foals and calves in Brazil and compare the isolated strains with S. Ndolo previously identified in asymptomatic reptiles. The affected calves and foals presented fever, lethargy, and profuse diarrhea. Isolated strains were subjected to antimicrobial susceptibility testing, characterized according to virulence genes, and fingerprinted by ERIC-PCR. Salmonella Ndolo was identified in fecal samples from two foals and four calves. One isolate from a calf was resistant to amoxicillin/clavulanic acid, trimethoprim/sulfamethoxazole, and florfenicol. Strains from two other calves were resistant to oxytetracycline. All... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonellosis; Zoonoses; Antimicrobial resistance; ERIC-PCR; Salmonella Ndolo.. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001200452 |
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